The qScript™One-Step SYBR® Green qRT-PCR Kit is a convenient and highly sensitive solution for quantitative RT-PCR of RNA templates (qRT-PCR) using SYBR® Green I dye detection and gene-specific primers

• Sensitive RNA detection with performance-engineered, RNase H (+) M-MLV reverse transcriptase
• Stringent ultra-pure antibody hot start ensures precise target amplification
• Supports either conventional or fast thermal cycling conditions

Real-Time PCR Instrument Compatibility:
Roche LightCycler™ 480
Qiagen/Corbett Rotor-Gene® Q
Qiagen/Corbett Rotor-Gene® 6000
Qiagen/Corbett Rotor-Gene® 3000
Eppendorf Mastercycler® ep realplex
Eppendorf Mastercycler® ep realplex2 s
Cepheid SmartCycler®
Bio-Rad CFX96™
Bio-Rad CFX384™
Bio-Rad/MJ Opticon®
Bio-Rad/MJ Opticon 2
Bio-Rad/MJ Chromo4™
Bio-Rad MiniOpticon™
Illumina Eco qPCR
Thermo Scientific PikoReal Cycler

Contents:
- qScript One-Step Reverse Transcriptase - Optimized 50X formulation of recombinant MMLV reverse transcriptase for one-step RT-PCR.
- One-Step SYBR Green Master Mix (2X) - 2X reaction buffer containing dNTPs, magnesium chloride, AccuStart Taq DNA polymerase, stabilizers, and SYBR Green I dye
- Nuclease-free water

Storage conditions:
Kit components are stable for one year when stored in a constant temperature freezer at 20ºC, protected from light. For convenience, the One-Step SYBR Green Master Mix may be stored unfrozen at +2 to +8ºC for up to 4 months.

Precautions and Disclaimer
For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. The system has been optimized to deliver maximum RT PCR efficiency, sensitivity, and specificity. The proprietary reaction buffer has been specifically formulated to maximize activities of both reverse transcriptase and Taq DNA polymerase while minimizing the potential for primer-dimer and other non-specific PCR artifacts. The kit is compatible with both fast and standard qPCR cycling protocols. Highly specific amplification is essential for successful qRT-PCR with SYBR® Green I technology, since this dye binds to any dsDNA generated during amplification. AccuStart™ Taq DNA polymerase contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation at 95ºC, the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase.