3,3',5,5'-Tetramethylbenzidine (TMB) solution, high sensitivity ready-to-use
About this item
Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is used with peroxidase and peroxidase coupled systems, particularly in ELISA techniques, blue result
Recommended for ELISA (microwell) procedures, 3,3',5,5'-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3',5,5'-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
The TMB solution has similar sensitivity levels to chemiluminescent detection reagents; it can detect as little as 0.15 ng. The TMB solution provided is enough for at least 25 mini-gel sized (10 X 10 cm) blots. Store at 2-8°C. Keep the substrate out of direct sunlight.
- CAS Number:54827-17-7
- Formula:C₁₆H₂₀N₂
- MDL Number:MFCD00007748
- Molecular Weight:240.35 g/mol
- Storage Temperature:Ambient
- EINECS:259-364-6
- UN:2810
- ADR:6.1,III
- ADR:"6.1",III
5 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is used with peroxidase and peroxidase coupled systems, particularly in ELISA techniques, blue result
Recommended for ELISA (microwell) procedures, 3,3',5,5'-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3',5,5'-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
The TMB solution has similar sensitivity levels to chemiluminescent detection reagents; it can detect as little as 0.15 ng. The TMB solution provided is enough for at least 25 mini-gel sized (10 X 10 cm) blots. Store at 2-8°C. Keep the substrate out of direct sunlight.
- CAS Number:54827-17-7
- Formula:C₁₆H₂₀N₂
- MDL Number:MFCD00007748
- Molecular Weight:240.35 g/mol
- Storage Temperature:Ambient
- EINECS:259-364-6
- UN:2810
- ADR:6.1,III
- ADR:"6.1",III
Documents
Product Family Options
Product Information
- SizeAvailabilityPrice
- Supplier Number: J61325-EQE
- Supplier Number: J61325-AP
- Supplier Number: J61325-AUItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
Life Science Promotion
Product Information
- SizeAvailabilityPrice
- Supplier Number: J61325-APSpecifications:New ProductNew Product
- Supplier Number: J61325-AUItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:New ProductNew Product
Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
Recommendations will be personalized based on your shopping preferences only if you have given your consent by enabling "Enhance my Shopping Experience" on the "Personal Info page".
Otherwise, you will receive generic recommendations.



