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Anti-NSF Mouse Monoclonal Antibody [clone: 9G7-3]
  89359-352
 :  Genetex
Anti-NSF Mouse Monoclonal Antibody [clone: 9G7-3]
  89359-352
 :  Genetex
 :  GTX12368

 

  • Antibody Type:
    Primary
  • Antigen Name:
    N-ethylmaleimide-sensitive Factor
  • Antigen Symbol:
    NSF
  • Clonality:
    Monoclonal
  • Clone:
    9G7-3
  • Conjugation:
    Unconjugated
  • Host:
    Mouse
  • ImmunoPrecipitation:
    Yes
  • Isotype:
    IgG2b
  • Reactivity:
    Bovine
  • Western Blot:
    Yes
  • Size:
    50 μg
  • Gene ID:
    504457
  • Antigen Synonyms:
    Anti-NSF|SKD2|N-ethylmaleimide-sensitive Fusion Protein|NSF
  • Storage Buffer:
    Preservative: NoneConstituents: 50% Glycerol, PBS, 0.1mM PMSF
  • Storage Temperature:
    Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
  • Concentration:
    1 mg/ml
  • Immunogen:
    Recombinant full length protein (Cow).
  • Tested Applications:
    IP, WB
  • Purification:
    Protein G purified
  • Cat. No.:
    89359-352
  • Supplier no.:
    GTX12368

 

 

N ethylmaleimide sensitive factor (NSF) is an ~83 kDa protein (predicted MW) which was initially isolated based on its ability to restore intercisternal Golgi transport to N ethylmaleimide treated membranes. NSF was subsequently discovered to be involved in a variety of membrane fusion events. NSF is an ATPase with two ATP binding domains that are essential for membrane fusion and homo oligomerization. In order to interact with target membranes, NSF requires another set of soluble proteins called Soluble NSF attachment proteins (SNAPs). NSF has been shown to play a prominent role in synaptic vesicle fusion. Together with synaptotagmin and a SNAP, NSF modulates the interaction of SNAP25, VAMP, and syntaxin, in an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion.

IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.25 ?g/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 82.6 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: NSF
Clonality: Monoclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b
Reactivity: Bovine