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Specifications
- Description:EZClick™ Global Protein Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence
- Size:100 assays
- Cat. No.:76200-046
Specifications
About this item
Assay to screen/study/characterize thioredoxin 1 inhibitors.
- Rapid, simple and sensitive
- Convenient and HTS
- Includes inhibitor control
The human Thioredoxin 1 (Trx1) is a key enzyme that protects cells from oxidative stress by reducing disulfides and methionine sulfoxide groups in oxidized proteins. It also regulates DNA repair and apoptosis. In mammals, Trx1 is necessary for growth and development. However, an aberrant elevated expression of Trx1 is known to promote lung, cervical, pancreatic, colorectal, and breast cancers.
The screening kit, Trx1 reduces quenched di-FITC-labeled oxidized glutathione (Trx1 substrate), to mono-FITC-labeled unquenched reduced glutathione. In the presence of a Trx1 inhibitor (control inhibitor included), the reaction is impeded resulting in lower or no signal. The assay is high-throughput adaptable and can be completed in less than 1 hr.