You searched for: Western Blotting Reagents

Supplier: Cytiva

Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.

Supplier: Cytiva

Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

Supplier: VWR

VisiGlo HRP and VisiGlo Plus HRP are luminol-based chemiluminescent substrates designed for the rapid and sensitive detection of peroxidase-labeled conjugates.

Supplier: Cytiva

Chemiluminescence detection reagent for detection of high to medium abundant proteins.

Supplier: Azure Biosystems

Azure Protein Free Blot Blocking Buffer is a protein free blocking solution, optimized for use with fluorescent Western blots, and also an excellent blocking reagent for chemiluminescent Western blotting.

Supplier: Cytiva

Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

Supplier: Azure Biosystems

Azure IR Fluorescent Blot Washing Buffer is optimized for use with visible and infrared fluorescent Western blotting.

Supplier: Azure Biosystems

Radiance Plus is the most sensitive HRP substrate for chemiluminescent western blotting available from Azure.

Supplier: Azure Biosystems

Radiance Q chemiluminescent substrate sets the bar for sensitivity and quantitative ability

Supplier: Azure Biosystems

Azure’s proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

Supplier: Azure Biosystems

Azure Blot Washing Buffer can be used with any Western blotting system.

Supplier: Cytiva

CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.

Supplier: RPI

Nonfat dry milk for use as a blocking agent during western blotting to block non-specific antibody binding and reduce background signal.

Supplier: Rockland Immunochemical

Nonradioactive, enhanced luminol-based chemiluminescent HRP substrates are designed for the detection of HRP (horseradish peroxidase) through western blotting and EIA (enzyme immunoassay) use

Supplier: Cytiva

Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.

Supplier: Cytiva

Amersham ECL Blocking Agent is for blocking nitrocellulose and PVDF membranes to be used with Amersham ECL, Amersham ECL Prime and Amersham ECL Select Western Blotting Detection Reagents

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.

Supplier: Cytiva

Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

Supplier: Cytiva

Cy™ fluorescent amidites for incorporation of Cy3, Cy3.5, Cy5, Cy5.5 fluorescent dyes at any position in an oligonucleotide during synthesis.

Supplier: Cytiva

Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.

Supplier: Enzo Life Sciences

Used in conjunction with peroxidase-based immunostaining systems.

Supplier: Enzo Life Sciences

Species Independent

Supplier: Enzo Life Sciences

Whole-cell extract of human HL-60 leukemia cells.

Supplier: Cytiva

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

ECL Direct™ nucleic acid labelling and detection systems are based on the direct labelling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalysed breakdown of luminol. Suitable for fast and easy detection of medium to high target amounts in applications such as colony/plaque screens, dot blots and PCR product analyses.

Supplier: Cytiva

Amersham ECL Plex™ Western blotting combination packs offer a complete system for fluorescent Western blotting, with improved formulation Cy3 conjugates for Lower background.

Supplier: Rockland Immunochemical

(3,3'-Diaminobenzidine) 0.0694 M A widely used substrate for both immunoblotting and immunohistochemical staining techniques. Produces an insoluble brown end product insoluble in, alcohol. A suspected carcinogen. Multi-component system.

Supplier: Cytiva

AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).