You searched for: Western Blotting Reagents
Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Western ReProbe™ Stripping Solution, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Western ReProbe™ is a single component system, specifically formulated to dissociate and remove antibodies from membrane bound proteins without destroying the antigenic binding affinity and does not use denaturants, SDS or boiling
Expand 6 Items
Advansta WesternBright™ Quantum HRP Substrate Kits
Supplier: Advansta
WesternBright Quantum is a new chemiluminescent substrate specially formulated for CCD imaging. This novel horseradish peroxidase, HRP, substrate provides a strong, long-lasting signal, the broadest useful linear range and high sensitivity for the most quantitative chemiluminescent Western assays.
Expand 3 Items
Saponin Permeating Solution, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Saponin permeating solution, is used as a cell permeabilizing agent.
Expand 1 Items
Advansta ELISABright™ Chemiluminescent Substrate for ELISA Applications
Supplier: Advansta
ELISABright is an enhanced and highly sensitive chemiluminescent HRP substrate optimized for ELISA applications. This luminol-based substrate demonstrates a wide linear dynamic range and a superior signal-to-noise ratio. ELISABright allows for enhanced detection of low abundance proteins and reduced consumption of antibodies
Expand 3 Items
AttoPhos® AP Fluorescent Substrate System, Promega
Supplier: Promega Corporation
A highly sensitive, flluorescent alkaline phosphatase substrate.
Expand 2 Items
Radiance ECL, Azure Biosystems
Supplier: Azure Biosystems
Radiance ECL is an HRP substrate with long lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other ECL substrates.
Expand 1 Items
FLASHBlot™ Transfer Buffers, Advansta
Supplier: Advansta
Increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins
Expand 2 Items
Pierce Miser™ Western Blotting Detection Reagents, Antibody Extender Solution NC, Thermo Scientific
Supplier: Invitrogen
Pierce Miser™ Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.
Expand 1 Items
Pierce™ Fast Western Blot Kit, ECL Substrate, Thermo Scientific
Supplier: Invitrogen
The Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.
Expand 2 Items
Restore™ Fluorescent Western Blot Stripping Buffer, Thermo Scientific
Supplier: Invitrogen
The Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.
Expand 2 Items
Pierce™ ECL Western Blotting Substrate, Thermo Scientific
Supplier: Invitrogen
A value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
Expand 3 Items
Pierce™ Substrate for Western Blotting, ECL Plus, Thermo Scientific
Supplier: Invitrogen
The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
Expand 1 Items
Pierce 1-Step™ Chloronaphthol Substrate Solution, Thermo Scientific
Supplier: Invitrogen
The 1-Step™ chloronaphthol solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.
Expand 1 Items
BioFX® Chemiluminescent Ultra Sensitive HRP Membrane Substrate, Surmodics IVD, Inc.
Supplier: Surmodics
BioFX® Chemiluminescent Ultra Sensitive HRP Membrane Substrate is a luminol based formulation for western blot applications.
Expand 1 Items
BioFX® Chemiluminescent Ultra Sensitive HRP Microwell Substrate, Surmodics IVD, Inc.
Supplier: Surmodics
Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in Western Blot assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the luminol substrate, antibody/antigen titers and blockers.
Expand 1 Items
SuperSignal™ West HisProbe Kit, Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific SuperSignal West HisProbe Kit contains HisProbe-HRP Conjugate, as well as reagents required for detection of polyhistidine-tagged protein by Western blotting
Expand 1 Items
BrightStar™ Duration HRP Chemiluminescent Substrates
Supplier: Alkali Scientific
Achieve sensitive detection in chemiluminescence assays with the BrightStar™ Duration HRP Chemiluminescent Substrate, designed for accurate and efficient protein detection.
Expand 2 Items
BioFX® Chemiluminescent Ultra Sensitive AP Microwell and/or Membrane Substrate (450 nm), Surmodics
Supplier: Surmodics
Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in ELISA and membrane assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the dioxetane substrate, antibody/antigen titers and blockers. Surmodics provides multiple 1-component AP chemiluminescent substrates with various intensities and wavelength emissions to allow the assay developer to choose the appropropriate substrate for the application.
Expand 1 Items
Pierce™ Guardian™ Peroxidase Conjugate Stabilizer/Diluent, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Guardian Peroxidase Conjugate Stabilizer/Diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long-term storage.
Expand 3 Items
Pierce™ LightShift™ Chemiluminescent RNA EMSA Kit, Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
Expand 1 Items
Pierce 1-Step™ NBT/BCIP plus Suppressor Substrate Solution, Thermo Scientific
Supplier: Invitrogen
The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
Expand 1 Items
Pierce™ Western Blotting Kits, Far-Western Blot Kit for Biotinylated Proteins, Thermo Scientific
Supplier: Invitrogen
The Pierce™ Far-Western Blot Kit for Biotinylated Proteins provide a nonisotopic approach to far-Western analysis that can be performed either directly in the gel or on the membrane
Expand 1 Items
Pierce™ 1-Step™ Ultra TMB-Blotting Solution, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.
Expand 1 Items
Clarity™ Western ECL Substrate, Chemiluminescent Detection, Bio-Rad
Supplier: Bio-Rad
The Bio-Rad Clarity™ Western ECL Substrate, for chemiluminescent western blot detection, is compatible with any HRP-conjugate secondary antibody and is compatible with both digital and film-based imaging.
Expand 2 Items
BioFX® Chemiluminescent Sensitive Plus HRP Microwell and/or Membrane Substrate, Surmodics IVD
Supplier: Surmodics
Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in ELISA and Western Blot assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the luminol substrate, antibody/antigen titers and blockers. Surmodics provides multiple 2-component HRP chemiluminescent substrates with various intensities to allow the assay developer to choose the appropropriate substrate for the application.
Expand 1 Items
Pierce Qentix™ Western Blot Signal Enhancer, Thermo Scientific
Supplier: Invitrogen
Western blot signal enhancer is used to pre-treat either nitrocellulose or PVDF membranes for a 3 – 10X increase in signal intensity for most target proteins.
Expand 1 Items
Pierce™ LightShift® Chemiluminescent EMSA Kit, Thermo Scientific
Supplier: Invitrogen
LightShift® Chemiluminescent EMSA Kit is robust and sensitive system for performing electrophoretic mobility shift assays (EMSAs) to identify and characterize protein:DNA binding interactions.
Expand 2 Items
Pierce™ CN/DAB Substrate Kit, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.
Expand 1 Items
Pierce 1-Step™ NBT/BCIP Substrate Solution, Thermo Scientific
Supplier: Invitrogen
The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
Expand 1 Items
WesternBright ECL HRP Substrate, Advansta
Supplier: ADVANSTA INC MS
Advansta's HRP substrate is the best choice for routine, film-imaged chemiluminescent Western blots.