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Protein Extraction Reagents

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

CHO/K1 Whole Cell Lysate, Rockland Immunochemical

Supplier: Rockland Immunochemical

Ready-to-use whole cell lysates are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.

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FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences

FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates.

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SMART Digest™ Protein Digests Kits

SMART Digest™ Protein Digests Kits

Supplier: Thermo Scientific

Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits.

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FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences

FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).

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Lysing Matrix Z, MP Biomedicals

Lysing Matrix Z, MP Biomedicals

Supplier: MP Biomedicals

Lysing Matrix Z is used for breaking tough plant and animal samples.

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BugBuster® HT Protein Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster® HT Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E

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VersaLyse™ Lysing Solution, 100 Tests, RUO

Supplier: Beckman Coulter

VersaLyse Lysing solution is a reagent used to lyse red blood cells from any biological fluid and, in particular, to lyse erythrocytes from whole blood. VersaLyse is a highly specific, very gentle lysing solution, suitable for applications such as immunophenotyping, cell sorting, activation or cell culture. It is designed for use in flow cytometry and facilitates obtaining highly discriminative scattergrams whatever the type of flow cytometer used. VersaLyse is a versatile reagent which may be used in lyse/wash, wash/lyse and lyse/no-wash procedures with undiluted or diluted samples. The histograms are dual-parameter light scatter histograms (FS versus SS) of a normal K3EDTA anticoagulated blood sample lysed with VersaLyse Lysing Solution (No-wash procedure).

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BugBuster® Protein Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster® Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E.coli, resulting in the liberation of soluble protein.

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PopCulture® Reagent, MilliporeSigma

Supplier: MilliporeSigma

PopCulture Reagent* is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium.

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BugBuster® (primary amine-free) Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilization or cross-linking.

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BugBuster® 10X Protein Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster 10X is a concentrated formulation of the proprietary detergents employed in BugBuster without the addition of salts or buffer components.

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PROTEIN GRADE® Detergent, C12E9, Calbiochem®, MilliporeSigma

Supplier: MilliporeSigma

A non-ionic detergent used for the solubilization of membrane-bound proteins in their native state.

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NP-40 Alternative, MilliporeSigma

Supplier: MilliporeSigma

NP-40 alternative is a non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis.

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Lysonase™ Bioprocessing Reagent, EMD Millipore

Supplier: MilliporeSigma

Lysonase™ bioprocessing reagent is an optimized, ready-to-use blend of rLysozyme™ solution and benzonase nuclease.

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Q-sep® QuEChERS Extraction Salts, Restek

Q-sep® QuEChERS Extraction Salts, Restek

Supplier: Restek

QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.

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Pepsin Reagent, Ready To Use, Electron Microscopy Science

Supplier: Electron Microscopy Sciences

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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Lumi Super Tracker™ Membrane/Blotting Chemiluminescent Peroxidase Substrate, ImmunO4

Supplier: Immuno4 LLC

Developed specifically for membrane/blotting applications requiring ultra sensitivity with exceptional signal to noise ratios.

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Lightning in a Bottle™, Chemiluminescent Membrane/Blotting Peroxidase Substrate

Supplier: Immuno4 LLC

Developed specifically as a ready to use reagent for Blotting applications requiring ultra sensitivity with exceptional signal to noise ratios

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Pronase Reagent, Ready To Use, Electron Microscopy Science

Supplier: Electron Microscopy Sciences

Pronase is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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Biotin Resin for Isolation of Avidin, Streptavidin & Neutravidin Coupled Molecules, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.

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Lumi Super Tracker™ Soluble/ELISA Chemiluminescent Peroxidase Substrate, ImmunO4

Supplier: Immuno4 LLC

Developed specifically for ELISA and In-Vitro Diagnostic applications requiring ultra sensitivity with exceptional signal to noise ratios.

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Iminobiotin Resin for Isolation of Avidin, Streptavidin and Neutravidin Complexes, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose

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Factor Xa Protease, New England Biolabs

Factor Xa Protease, New England Biolabs

Supplier: New England Biolabs (NEB)

Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg

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Protein Reducing Agents, G-Biosciences

Protein Reducing Agents, G-Biosciences

Supplier: G-Biosciences

A water soluble, odorless, non-toxic and stable protein reductant, G-Biosciences' Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol)

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ImmunoHistoZyme™, Ready-to-Use, Electron Microscopy Sciences

Supplier: Electron Microscopy Sciences

This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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ProteaseMAX™ Surfactant, Trypsin Enhancer, Promega

ProteaseMAX™ Surfactant, Trypsin Enhancer, Promega

Supplier: Promega Corporation

ProteaseMAX™ surfactant, trypsin enhancer enhances the enzymatic performance of trypsin, chymotrypsin and Lys-C in digestion reactions.

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Trypsin/Lys-C Mix, MS Grade, Promega

Trypsin/Lys-C Mix, MS Grade, Promega

Supplier: Promega Corporation

Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.

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B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

Supplier: Invitrogen

B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.

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Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific

Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.

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ReadyPrep Reagents for Extraction and Solubilization, Bio-Rad

ReadyPrep Reagents for Extraction and Solubilization, Bio-Rad

Supplier: Bio-Rad

A number of ReadyPrep kits are available for total protein or targeted protein extraction (fractionation)

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