Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.
CHO/K1 Whole Cell Lysate, Rockland Immunochemical
Supplier: Rockland Immunochemical
Ready-to-use whole cell lysates are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.
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FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences
Supplier: Enzo Life Sciences
FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates.
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SMART Digest™ Protein Digests Kits
Supplier: Thermo Scientific
Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits.
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FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences
Supplier: Enzo Life Sciences
FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).
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Lysing Matrix Z, MP Biomedicals
Supplier: MP Biomedicals
Lysing Matrix Z is used for breaking tough plant and animal samples.
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BugBuster® HT Protein Extraction Reagent, MilliporeSigma
Supplier: MilliporeSigma
BugBuster® HT Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E
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VersaLyse™ Lysing Solution, 100 Tests, RUO
Supplier: Beckman Coulter
VersaLyse Lysing solution is a reagent used to lyse red blood cells from any biological fluid and, in particular, to lyse erythrocytes from whole blood. VersaLyse is a highly specific, very gentle lysing solution, suitable for applications such as immunophenotyping, cell sorting, activation or cell culture. It is designed for use in flow cytometry and facilitates obtaining highly discriminative scattergrams whatever the type of flow cytometer used. VersaLyse is a versatile reagent which may be used in lyse/wash, wash/lyse and lyse/no-wash procedures with undiluted or diluted samples. The histograms are dual-parameter light scatter histograms (FS versus SS) of a normal K3EDTA anticoagulated blood sample lysed with VersaLyse Lysing Solution (No-wash procedure).
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BugBuster® Protein Extraction Reagent, MilliporeSigma
Supplier: MilliporeSigma
BugBuster® Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E.coli, resulting in the liberation of soluble protein.
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PopCulture® Reagent, MilliporeSigma
Supplier: MilliporeSigma
PopCulture Reagent* is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium.
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BugBuster® (primary amine-free) Extraction Reagent, MilliporeSigma
Supplier: MilliporeSigma
BugBuster (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilization or cross-linking.
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BugBuster® 10X Protein Extraction Reagent, MilliporeSigma
Supplier: MilliporeSigma
BugBuster 10X is a concentrated formulation of the proprietary detergents employed in BugBuster without the addition of salts or buffer components.
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PROTEIN GRADE® Detergent, C12E9, Calbiochem®, MilliporeSigma
Supplier: MilliporeSigma
A non-ionic detergent used for the solubilization of membrane-bound proteins in their native state.
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NP-40 Alternative, MilliporeSigma
Supplier: MilliporeSigma
NP-40 alternative is a non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis.
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Lysonase™ Bioprocessing Reagent, EMD Millipore
Supplier: MilliporeSigma
Lysonase™ bioprocessing reagent is an optimized, ready-to-use blend of rLysozyme™ solution and benzonase nuclease.
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Q-sep® QuEChERS Extraction Salts, Restek
Supplier: Restek
QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.
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Pepsin Reagent, Ready To Use, Electron Microscopy Science
Supplier: Electron Microscopy Sciences
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
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Lumi Super Tracker™ Membrane/Blotting Chemiluminescent Peroxidase Substrate, ImmunO4
Supplier: Immuno4 LLC
Developed specifically for membrane/blotting applications requiring ultra sensitivity with exceptional signal to noise ratios.
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Lightning in a Bottle™, Chemiluminescent Membrane/Blotting Peroxidase Substrate
Supplier: Immuno4 LLC
Developed specifically as a ready to use reagent for Blotting applications requiring ultra sensitivity with exceptional signal to noise ratios
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Pronase Reagent, Ready To Use, Electron Microscopy Science
Supplier: Electron Microscopy Sciences
Pronase is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
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Biotin Resin for Isolation of Avidin, Streptavidin & Neutravidin Coupled Molecules, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.
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Lumi Super Tracker™ Soluble/ELISA Chemiluminescent Peroxidase Substrate, ImmunO4
Supplier: Immuno4 LLC
Developed specifically for ELISA and In-Vitro Diagnostic applications requiring ultra sensitivity with exceptional signal to noise ratios.
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Iminobiotin Resin for Isolation of Avidin, Streptavidin and Neutravidin Complexes, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose
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Factor Xa Protease, New England Biolabs
Supplier: New England Biolabs (NEB)
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg
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Protein Reducing Agents, G-Biosciences
Supplier: G-Biosciences
A water soluble, odorless, non-toxic and stable protein reductant, G-Biosciences' Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol)
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ImmunoHistoZyme™, Ready-to-Use, Electron Microscopy Sciences
Supplier: Electron Microscopy Sciences
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
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ProteaseMAX™ Surfactant, Trypsin Enhancer, Promega
Supplier: Promega Corporation
ProteaseMAX™ surfactant, trypsin enhancer enhances the enzymatic performance of trypsin, chymotrypsin and Lys-C in digestion reactions.
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Trypsin/Lys-C Mix, MS Grade, Promega
Supplier: Promega Corporation
Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.
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B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific
Supplier: Invitrogen
B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.
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Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.
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ReadyPrep Reagents for Extraction and Solubilization, Bio-Rad
Supplier: Bio-Rad
A number of ReadyPrep kits are available for total protein or targeted protein extraction (fractionation)