Protein Extraction Reagents
Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.
ProteaseMAX™ Surfactant, Trypsin Enhancer, Promega
Supplier: Promega Corporation
ProteaseMAX™ surfactant, trypsin enhancer enhances the enzymatic performance of trypsin, chymotrypsin and Lys-C in digestion reactions.
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Accessories for InGel™ Silver for Protein Digestion, G-Biosciences
Supplier: G-Biosciences
Suitable for 100 protein spots. provides optimal buffered conditions for in gel trypsin digestion of proteins.
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Lumi Super Tracker™ Soluble/ELISA Chemiluminescent Peroxidase Substrate, ImmunO4
Supplier: Immuno4 LLC
Developed specifically for ELISA and In-Vitro Diagnostic applications requiring ultra sensitivity with exceptional signal to noise ratios.
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Iminobiotin Resin for Isolation of Avidin, Streptavidin and Neutravidin Complexes, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose
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VersaLyse™ RBC Lysing Solution, Beckman Coulter
Supplier: Beckman Coulter
VersaLyse lysing solution is intended for the lysis of red blood cells in the preparation of biological samples for flow cytometry analysis.
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Pierce™ Endoproteinase Chymotrypsin Protease (TLCK treated), MS Grade, Thermo Scientific
Supplier: Invitrogen
Chymotrypsin Protease, MS Grade, is a purified native endoproteinase validated for maximum activity and selectivity in proteomic and used in in-solution digestions and in-gel digestion of proteins from from 1-D or 2-D gels. Store at -20˚C in a nonfrost-free freezer.
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Pierce™ Endoproteinase Asp-N Protease, MS Grade, Thermo Scientific
Supplier: Invitrogen
Asp-N Protease, MS Grade, is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications. Store at -20˚C in a nonfrost-free freezer.
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Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.
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Pierce™ Endoproteinase Glu-C Protease, MS Grade, Thermo Scientific
Supplier: Invitrogen
Glu-C Protease, MS Grade, is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications, in-solution digestion of proteins and in-gel digestion of proteins. Store at -20 ˚C in a nonfrost-free freezer.
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Pierce™ Micrococcal Nuclease Solution, Thermo Scientific
Supplier: Invitrogen
The Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
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ReadyPrep Reagents for Extraction and Solubilization, Bio-Rad
Supplier: Bio-Rad
A number of ReadyPrep kits are available for total protein or targeted protein extraction (fractionation)
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Pierce™ Immobilized Pepsin (Agarose Resin), Thermo Scientific
Supplier: Invitrogen
Immobilized Pepsin agarose consists of an acidic endopeptidase that has been immobilized onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.
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Pierce Imject™ Maleimide-Activated Ovalbumin, Thermo Scientific
Supplier: Invitrogen
Imject™ Maleimide-activated ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.
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Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.
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Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific
Supplier: Invitrogen
Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling
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Pierce™ Immobilized Papain (Agarose Resin), Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Immobilized Papain consists of a cysteine-endopeptidase (papain) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
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