Protein Extraction Reagents

Supplier: Abcam

Trypsin Antigen Retrieval Kit ab259256 is used for enzyme-based antigen retrieval.

Supplier: Thermo Scientific

Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

Supplier: Abcam

This product is manufactured by BioVision, an Abcam company and was previously called 8002 EZLys™ Tissue Protein Extraction Reagent.

Supplier: Thermo Scientific

Pierce™ Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss.

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates.

Supplier: Abcam

Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970).

Supplier: Thermo Scientific

The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).

Supplier: Abcam

Proteolytic antigen retrieval (PIER) reagent HistoReveal (ab103720).

Supplier: MP Biomedicals

Lysing Matrix Z is used for breaking tough plant and animal samples.

Supplier: Abcam

FASP Protein Digestion Kit (FFPE) (ab270543) allows unbiased extraction, complete proteome solubilization, and highly efficient digestion of FFPE tissue.

Supplier: MilliporeSigma

PopCulture Reagent* is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium.

Supplier: MilliporeSigma

BugBuster® HT Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E

Supplier: MilliporeSigma

NP-40 alternative is a non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis.

Supplier: MilliporeSigma

BugBuster 10X is a concentrated formulation of the proprietary detergents employed in BugBuster without the addition of salts or buffer components.

Supplier: MilliporeSigma

BugBuster (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilization or cross-linking.