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Protein Purification Reagents

Protein purification reagents are for use in isolating one or more proteins from a complex mixture, usually cells, tissues, or whole organisms. This process is vital for function and structure characterization, as well as identifying interactions of the protein of interest. Important during the many steps, the protein purification reagents assist with cell lysis, separating the soluble protein components from cell debris, and finally separating the protein of interest from product- and process-related impurities.

PureCube 100 Compact Cartridge Ni-INDIGO

PureCube 100 Compact Cartridge Ni-INDIGO

Supplier: Cube Biotech

The PureCube Ni-INDIGO column/cartridge is an IMAC chromatography column prepacked with PureCube 100 INDIGO-Ni Agarose resin beads.

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PureCube Ni-IDA Agarose XL

PureCube Ni-IDA Agarose XL

Supplier: Cube Biotech

Our PureCube Ni-IDA XL agarose resins are agarose resin beads with a diameter of ∼400 µm. They are used to purify active His-tagged proteins from cells and extremely viscous cell media.

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PureCube 100 NTA Agarose

PureCube 100 NTA Agarose

Supplier: Cube Biotech

PureCube NTA (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods. The beads in this suspension have an average diameter of 100 µm.

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PureCube 1-Step Batch MINI

PureCube 1-Step Batch MINI

Supplier: Cube Biotech

Empty 1-step batch columns are ideally suited for batch purifications. They can be filled with your preferred affinity resin, allowing binding, washing, and elution in one spin column.

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PureCube Fe-NTA Agarose

PureCube Fe-NTA Agarose

Supplier: Cube Biotech

PureCube Fe-NTA agarose resin beads were developed for the enrichment of phosphorylated proteins.

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PureCube Al-NTA Agarose

PureCube Al-NTA Agarose

Supplier: Cube Biotech

PureCube Al-NTA agarose resin beads were developed for the enrichment of phosphorylated proteins.

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PureCube 100 Compact Cartridge Co-NTA

PureCube 100 Compact Cartridge Co-NTA

Supplier: Cube Biotech

The PureCube 100 Co-NTA compact cartridge is an IMAC chromatography column prepacked with PureCube 100 Co-NTA resin.

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PureCube 100 Compact Cartridge Ni-NTA

PureCube 100 Compact Cartridge Ni-NTA

Supplier: Cube Biotech

The PureCube 100 Ni-NTA compact cartridge is an IMAC chromatography column prepacked with PureCube 100 Ni-NTA resin.

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PureCube Compact Cartridge Ni-NTA

PureCube Compact Cartridge Ni-NTA

Supplier: Cube Biotech

The PureCube Ni-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Ni-NTA resin.

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PureCube Maleimide Activated Agarose

PureCube Maleimide Activated Agarose

Supplier: Cube Biotech

PureCube Maleimide Agarose resin is your best option to couple biomolecules that carry thiol (SH) groups.

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Protein Concentrator Powder, G-Biosciences

Protein Concentrator Powder, G-Biosciences

Supplier: G-Biosciences

Concentrator Powder is a high molecular weight polymer which will not migrate across the dialysis membrane

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HisCube HisCube Ni-Indigo His-Tag Protein Purification MIDI Kit

HisCube HisCube Ni-Indigo His-Tag Protein Purification MIDI Kit

Supplier: Cube Biotech

The HisCube His-tag protein purification MIDI kit is our way to supply your laboratory with a convenient method for high-quality, small-scale His-tag protein purifications. We put all of our experience in His-tag purifications into this Kit to provide a protocol that is easy to follow.

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HisCube HisCube Ni-Indigo His-Tag Protein Purification MINI Kit

HisCube HisCube Ni-Indigo His-Tag Protein Purification MINI Kit

Supplier: Cube Biotech

The HisCube His-tag protein purification MINI kit is our way to supply your laboratory with a convenient method for high-quality, small-scale His-tag protein purifications. We put all of our experience in His-tag purifications into this Kit to provide a protocol that is easy to follow.

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PureCube 100 Co-NTA Agarose

PureCube 100 Co-NTA Agarose

Supplier: Cube Biotech

Our PureCube 100 Co-NTA agarose resins are agarose resin beads with a diameter of 100 µm. They are used for the purification of active His-tagged proteins from cells.

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PureCube Compact Cartridge Co-NTA

PureCube Compact Cartridge Co-NTA

Supplier: Cube Biotech

The PureCube Co-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Co-NTA resin.

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HighSpec HighSpec Rho1D4 Agarose XL

HighSpec HighSpec Rho1D4 Agarose XL

Supplier: Cube Biotech

HighSpec Rho1D4 agarose XL resin is a suspension of 400 µm agarose beads coupled to an anti-Rho1D4 antibody.

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PureCube 100 Ni-NTA Agarose

PureCube 100 Ni-NTA Agarose

Supplier: Cube Biotech

Our PureCube Ni-NTA agarose resins are your best option to purify His-tagged protein using FPLC, Batch spin, or column chromatography.

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PureCube Epoxy Activated Agarose

PureCube Epoxy Activated Agarose

Supplier: Cube Biotech

PureCube Epoxy Agarose resin is your best option to couple biomolecules that carry free-standing amine or thiol groups.

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PureCube Ni-NTA Agarose XL

PureCube Ni-NTA Agarose XL

Supplier: Cube Biotech

Our PureCube Ni-NTA agarose resins are your best option to purify His-tagged protein using FPLC, Batch spin, or column chromatography.

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Pierce™ Immobilized p-Aminophenyl Phosphoryl Choline Agarose, Thermo Scientific

Pierce™ Immobilized p-Aminophenyl Phosphoryl Choline Agarose, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce p-Amino Phosphoryl Choline Agarose enables purification of C-reactive protein (CRP) from plasma, ascites and other biological fluids.

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Pierce™ Phosphopeptide Enrichment Spin Tip, Thermo Scientific

Supplier: Invitrogen

TiO2 Phosphopeptide Enrichment Spin Tips, Formulation: 200uL pipette tips containing TiO2 resin; Sufficient For: Processing 96 digested protein samples (0.3-1mg each) containing up to 100ug of phosphopeptides each; 96 tips

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SulfoLink™ Coupling Resin, Thermo Scientific

SulfoLink™ Coupling Resin, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilization of cysteine-peptides and other sulfhydryl molecules.

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Pierce™ High Sensitivity Streptavidin-HRP, Thermo Scientific

Pierce™ High Sensitivity Streptavidin-HRP, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce High Sensitivity Streptavidin-HRP is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

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Pierce™ Pegylation Reagents, MS(PEG)n Methyl-PEG-NHS-Esters, Thermo Scientific

Pierce™ Pegylation Reagents, MS(PEG)n Methyl-PEG-NHS-Esters, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)12 is a three-branched, polyethylene glycol compound (3 x 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.

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Pierce™ Mass Spectrometry Reagents, MALDI Matrices, Single-Use, Thermo Scientific

Pierce™ Mass Spectrometry Reagents, MALDI Matrices, Single-Use, Thermo Scientific

Supplier: Invitrogen

MALDI Matrices are highly purified, recrystallized reagents packaged in convenient single-use tubes sufficient for analyzing up to 96 samples by matrix assisted laser desorption ionization.

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Pierce™ Inclusion Body Solubilization Reagent, Thermo Scientific

Pierce™ Inclusion Body Solubilization Reagent, Thermo Scientific

Supplier: Invitrogen

Inclusion Body Solubilization Reagent effectively retrieves recombinant proteins that form insoluble aggregates during bacterial overexpression.

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Pierce™ PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Thermo Scientific

Pierce™ PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Thermo Scientific

Supplier: Invitrogen

Pierce™ SAT(PEG)₄ (PEGylated N-succinimidyl S-acetylthioacetate) is a sulphhydryl-addition reagent for covalent modification of primary amines.

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Pierce™ Pegylation Reagents, Carboxyl-PEG-Thiol Compound, Thermo Scientific

Pierce™ Pegylation Reagents, Carboxyl-PEG-Thiol Compound, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce CT(PEG)12 or carboxy-PEG12-thiol, is a carboxyl- and sulfhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

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Pierce™ Pegylation Reagents, Methyl-PEG-NHS-Maleimide, Thermo Scientific

Pierce™ Pegylation Reagents, Methyl-PEG-NHS-Maleimide, Thermo Scientific

Supplier: Invitrogen

Pierce™ MM(PEG)n reagents are methyl-terminated PEG compounds activated with a maleimide group for covalent pegylation of sulfhydryls on proteins or assay surfaces.

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Pierce™ Protein Stabilizing Cocktail, Thermo Scientific

Pierce™ Protein Stabilizing Cocktail, Thermo Scientific

Supplier: Invitrogen

A versatile stabilizing solution that increases the shelf-life of purified or partially purified proteins during routine storage.

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