You searched for: Cloning Kits and Ligation

Supplier: Promega Corporation

For ligation of DNA ends, mutagenesis and cDNA cloning

Supplier: New England Biolabs (NEB)

The NEBNext® Ultra™ Ligation Module is designed for use with the NEBNext Singleplex or Multiplex Oligos for Illumina.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q) specifically ligates the preadenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Supplier: New England Biolabs (NEB)

Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer.

Supplier: New England Biolabs (NEB)

ElectroLigase™ combines T4 DNA ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG.

Supplier: Promega Corporation

For ligation of DNA ends.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, with hydrolysis of ATP to AMP and PPi

Supplier: New England Biolabs (NEB)

NEBuilder HiFi DNA Assembly was developed to improve the efficiency and accuracy of DNA assembly

Supplier: New England Biolabs (NEB)

SplintR Ligase, also known as PBCV-1 DNA Ligase or Chorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA oligonucleotides splinted by a complementary RNA strand.

Supplier: Promega Corporation

T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA.

Supplier: New England Biolabs (NEB)

9°N™ DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA

Supplier: New England Biolabs (NEB)

Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Supplier: New England Biolabs (NEB)

Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.

Supplier: New England Biolabs (NEB)

Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA containing cohesive ends.

Supplier: Promega Corporation

NM522 contains an F' episome, which is required for production of ssDNA and for blue/white color selection.

Supplier: New England Biolabs (NEB)

NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly.

Supplier: EMPIRICAL BIOSCIENCE

T4 DNA Ligase

Supplier: Promega Corporation

Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a ribosomal binding site. No blue/white selection.

Supplier: New England Biolabs (NEB)

RtcB Ligase is expressed as a His-tagged fusion in E. coli.

Supplier: New England Biolabs (NEB)

NEBridge Ligase Master Mix is a 3X master mix for Golden Gate Assembly. Designed for use with NEB Type IIS restriction enzymes, this master mix contains T4 DNA Ligase in an optimized reaction buffer with a proprietary ligation enhancer.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity

Supplier: New England Biolabs (NEB)

The Gibson Assembly Cloning Kit combines the power of the Gibson Assembly Master Mix with NEB 5-alpha Competent E.coli, enabling successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility and transformation in just under 2 hours.

Supplier: Promega Corporation

HaloTag Cloning Starter Kit: designed to use Flexi Cloning System to clone protein coding sequences into mammalian HaloTag expression vectors; clone and transfer gene sequences into C- or N-terminus of HaloTag Flexi CMV Deletion Vectors.

Supplier: Promega Corporation

The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.

Supplier: ReVacc Scientific

This Seamless Assembly Master Mix (2X) uses Gibson cloning principle to allow efficiently assembly of multiple DNA fragments, regardless of fragment length or end compatibility. The insert and the linearized vector need the overlapped sequences of 15 bp - 20 bp on both 5' - and 3' -end, respectively. A positive control with the mixture of insert and linearized vector is included.

Supplier: New England Biolabs (NEB)

T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7.

Supplier: New England Biolabs (NEB)

T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 1, High Concentration (M0437M), is a 3X higher concentration than our standard T4 RNA Ligase 1 (M0204S/L)