You searched for: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

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SPLINTR® Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)

SplintR Ligase, also known as PBCV-1 DNA Ligase or Chorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA oligonucleotides splinted by a complementary RNA strand.

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NEBridge Ligase Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)

NEBridge Ligase Master Mix is a 3X master mix for Golden Gate Assembly. Designed for use with NEB Type IIS restriction enzymes, this master mix contains T4 DNA Ligase in an optimized reaction buffer with a proprietary ligation enhancer.

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T7 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)

T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7.

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T3 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)

T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3.

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Seamless Assembly Master Mix (2X)

Supplier: ReVacc Scientific

This Seamless Assembly Master Mix (2X) uses Gibson cloning principle to allow efficiently assembly of multiple DNA fragments, regardless of fragment length or end compatibility. The insert and the linearized vector need the overlapped sequences of 15 bp - 20 bp on both 5' - and 3' -end, respectively. A positive control with the mixture of insert and linearized vector is included.

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Taq DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)

Taq DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA

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EnGen® Spy Cas9 HF1

Supplier: New England Biolabs (NEB)

EnGen® Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from streptococcus pyogenes with reduced non-specific DNA cleavage.

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ig-Fusion™ Cloning Kit, Intact Genomics

Supplier: INTACT GENOMICS, INC MS

Propriety ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR products to any linearized expression vector at any site.

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T4 DNA Ligase, Intact Genomics

Supplier: INTACT GENOMICS, INC MS

T4 DNA ligase displays significantly higher ligation efficiency than the nearest competitor.

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T4 DNA Ligase, Intact Genomics

Supplier: INTACT GENOMICS, INC MS

T4 DNA ligase displays significantly higher ligation efficiency than the nearest competitor.

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Ig-Fusion Cloning Enzyme Premix, Intact Genomics

Supplier: INTACT GENOMICS, INC MS

Intact Genomics propriety Ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site.