You searched for: Cloning Kits and Ligation

Supplier: New England Biolabs (NEB)

NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly.

Supplier: New England Biolabs (NEB)

The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature

Supplier: Promega Corporation

For ligation of DNA ends.

Supplier: EMPIRICAL BIOSCIENCE

T4 DNA Ligase

Supplier: New England Biolabs (NEB)

The Gibson Assembly Cloning Kit combines the power of the Gibson Assembly Master Mix with NEB 5-alpha Competent E.coli, enabling successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility and transformation in just under 2 hours.

Supplier: New England Biolabs (NEB)

Thermostable 5´ App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity

Supplier: New England Biolabs (NEB)

Gibson Assembly was developed by Dr

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 1, High Concentration (M0437M), is a 3X higher concentration than our standard T4 RNA Ligase 1 (M0204S/L)

Supplier: New England Biolabs (NEB)

Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer.

Supplier: New England Biolabs (NEB)

The NEBuilder HiFi DNA Assembly Cloning Kit combines the power of the NEBuilder HiFi DNA Assembly Master Mix with NEB 5-alpha Competent E

Supplier: New England Biolabs (NEB)

Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.

Supplier: Promega Corporation

The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.

Supplier: Promega Corporation

For ligation of DNA ends, mutagenesis and cDNA cloning.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q) specifically ligates the preadenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, with hydrolysis of ATP to AMP and PPi

Supplier: New England Biolabs (NEB)

Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA containing cohesive ends.

Supplier: New England Biolabs (NEB)

NEBuilder HiFi DNA Assembly was developed to improve the efficiency and accuracy of DNA assembly

Supplier: Promega Corporation

Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a ribosomal binding site. No blue/white selection.

Supplier: New England Biolabs (NEB)

The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.

Supplier: New England Biolabs (NEB)

RtcB Ligase is expressed as a His-tagged fusion in E. coli.

Supplier: Promega Corporation

HaloTag Cloning Starter Kit: designed to use Flexi Cloning System to clone protein coding sequences into mammalian HaloTag expression vectors; clone and transfer gene sequences into C- or N-terminus of HaloTag Flexi CMV Deletion Vectors.

Supplier: Promega Corporation

Useful host for transformation of pGEM Vectors and production of ssDNA from M18 or phagemid vectors. JM109 is recA- and lacks the E. coli K restriction system. Can be used for blue/white screening.

Supplier: New England Biolabs (NEB)

The NEBNext® Ultra™ Ligation Module is designed for use with the NEBNext Singleplex or Multiplex Oligos for Illumina.

Supplier: Promega Corporation

T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA.

Supplier: New England Biolabs (NEB)

SplintR Ligase, also known as PBCV-1 DNA Ligase or Chorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA oligonucleotides splinted by a complementary RNA strand.

Supplier: New England Biolabs (NEB)

NEBridge Ligase Master Mix is a 3X master mix for Golden Gate Assembly. Designed for use with NEB Type IIS restriction enzymes, this master mix contains T4 DNA Ligase in an optimized reaction buffer with a proprietary ligation enhancer.

Supplier: New England Biolabs (NEB)

T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7.

Supplier: New England Biolabs (NEB)

T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3.