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You searched for: Cell Culture Buffers

Cell Culture Buffers

Tissue culture buffers provide an energy source, maintain proper pH levels, and keep ideal osmotic pressure present. Used predominately in biomolecular research, the dry and liquid substances have a balanced inorganic salt composition. Lower concentration options permit equilibration with air in a closed system, while higher salt concentration options keep gaseous phases in equilibrium. Cell growth is optimal when plated in a nutritional medium that has been buffered.

10X Phosphate Buffered Saline (PBS), Sterile,  Dulbecco's Formula, without Magnesium and Calcium, MP Biomedicals

10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium, MP Biomedicals

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

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Phosphate Buffer

Phosphate Buffer

Supplier: Hardy Diagnostics

Phosphate Buffer

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Corning® Tris, 10X Buffered Saline, Liquid, Corning

Corning® Tris, 10X Buffered Saline, Liquid, Corning

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range

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Dulbecco's Phosphate Buffered Saline (DPBS), Quality Biological

Dulbecco's Phosphate Buffered Saline (DPBS), Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

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Wash Buffer Salts (3 packs), Enzo Life Sciences

Supplier: Enzo Life Sciences

Wash Buffer Salts can be used in tissue preparations, immunohistochemical staining procedures and in assays that require HRP-linked detection reagents

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Hanks' Balanced Salt Solution (HBSS), Quality Biological

Hanks' Balanced Salt Solution (HBSS), Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

Hanks' Balanced Salt Solution (HBSS) provides a buffering system to maintain the physiological pH range and osmotic balance of the culture media. HBSS provides cells with a source of water and essential inorganic ions, and carbohydrate as an energy source.

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Corning® EDTA, 0.5M pH 8.0, Corning

Corning® EDTA, 0.5M pH 8.0, Corning

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

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Neutralizing Buffer, Hardy Diagnostics

Neutralizing Buffer, Hardy Diagnostics

Supplier: Hardy Diagnostics

Neutralizing Buffer allows the bacteriostatic effects of chlorine and quaternary ammonia compounds

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PBS (Endotoxin-Free), Enzo Life Sciences

Supplier: Enzo Life Sciences

Sterile and endotoxin-free PBS for use with TLRgrade™ or endotoxin-free grade reagents.

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HBSS 10X-Hanks' Balanced Salt Solution

HBSS 10X-Hanks' Balanced Salt Solution

Supplier: FUJIFILM IRVINE SCIENTIFIC INC.

HBSS 10X Hanks' Balanced salt solution is designed to maintain pH and osmotic balance and to provide cells with water and essential inorganic ions. HBSS is suitable for use in sealed culture flasks incubated in air.

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Buffer No. 4

Buffer No. 4

Supplier: Hardy Diagnostics

For use in antibiotic potency assay.

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HBSS 1X-Hanks' Balanced Salt Solutions

HBSS 1X-Hanks' Balanced Salt Solutions

Supplier: FUJIFILM IRVINE SCIENTIFIC INC.

HBSS 1X Hanks' Balanced Salt Solution is designed to maintain pH and osmotic balance and to provide cells with water and essential inorganic ions. HBSS is suitable for use in sealed culture flasks incubated in air.

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Buffer #6, Hardy Diagnostics

Buffer #6, Hardy Diagnostics

Supplier: Hardy Diagnostics

For use in the determination of antimicrobial activity per USP.

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20X PBS (Phosphate Buffered Saline), pH 7.4

Supplier: GROWCELLS

Balanced salt solution used in many biomolecular applications

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Buffer No. 16, Hardy Diagnostics

Buffer No. 16, Hardy Diagnostics

Supplier: Hardy Diagnostics

For the use in determination of antimicrobial activity.

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STERI-Buffer®, Veltek Associates

STERI-Buffer®, Veltek Associates

Supplier: Veltek Associates

Developed for use as a buffer solution and diluent in lab testing such a bioburden testing, pharmaceutical microbial limits testing, and AET testing and as a rinsing agent."

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Phosphate Buffered Saline (PBS) with 0.02% Tween® 80

Supplier: Hardy Diagnostics

0.01 M Phosphate Buffered Saline with 0.02% Tween® 80 (PBST) is recommended for use in performing microbial sampling, culture, and surveillance procedures for reprocessed endoscopes.

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PBS (Phosphate Buffered Saline), 10X, pH 7.4, GrowCells

Supplier: GROWCELLS

Balanced salt solution used in many biomolecular applications

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Saline, 0.85%, Hardy Diagnostics

Saline, 0.85%, Hardy Diagnostics

Supplier: Hardy Diagnostics

For the preparation of isotonic suspensions and dilutions of microorganisms.

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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Supplier: MP Biomedicals

Used to minimize cell attachment or aggregation.

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Butterfield's Phosphate Buffer, Hardy Diagnostics

Butterfield's Phosphate Buffer, Hardy Diagnostics

Supplier: Hardy Diagnostics

For the preparation of dilutions for plate count and other laboratory tests.

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Corning® 3D Clear Washing Buffer 10X, Corning

Corning® 3D Clear Washing Buffer 10X, Corning

Supplier: Corning

Corning 3D Clear Washing Buffer 10X is used for the Corning 3D Clear immunolabeling process.

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Corning® 3D Clear Antibody Buffer, Corning

Corning® 3D Clear Antibody Buffer, Corning

Supplier: Corning

Corning 3D Clear Antibody Buffer used with immunolabeling in Corning 3D tissue labeling approach.

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1X PBS (Phosphate Buffered Saline), pH 7.4

Supplier: GROWCELLS

Balanced salt solution used in many biomolecular applications

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1X Hanks' Balanced Salt Solution (Modified) without Phenol Red, MP Biomedical

1X Hanks' Balanced Salt Solution (Modified) without Phenol Red, MP Biomedical

Supplier: MP Biomedicals

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.

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Corning® 10% SDS (Sodium Dodecyl Sulfate), Corning

Corning® 10% SDS (Sodium Dodecyl Sulfate), Corning

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

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1.0 M HEPES pH 7.2

Supplier: Rockland Immunochemical

HEPES is a zwitterionic organic chemical buffering agent.

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Corning® 3D Clear Penetration Buffer, Corning

Corning® 3D Clear Penetration Buffer, Corning

Supplier: Corning

Corning 3D Clear Penetration Buffer is used with the Corning 3D immunolabeling process

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Hanks' Balanced Salts, without Sodium Bicarbonate, MP Biomedical, LLC

Supplier: MP Biomedicals

Hanks' Balanced Salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

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Corning® 3D Clear Blocking Buffer, Corning

Corning® 3D Clear Blocking Buffer, Corning

Supplier: Corning

Corning 3D Clear Blocking Buffer used during the Corning 3D Clear immunolabeling process.

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