Phenol
Phenol (C₆H₆O) is an aromatic organic compound with mildly acidic and antiseptic properties. It appears as a crystalline solid that liquefies slightly above room temperature and is highly soluble in organic solvents. Phenol is widely used in laboratories as a reagent for protein and nucleic acid extraction and in chemical synthesis for the production of resins, plastics, and pharmaceuticals. Furthermore it is used as phenol–chloroform extraction to isolate DNA, RNA, and proteins from biological samples. Due to its corrosive and toxic nature, phenol must be handled under proper ventilation with suitable protective equipment.
Phenol ≥99.0%, crystalline, Ultra Pure Grade
Supplier: VWR
Phenol ≥99.0%, crystalline, Ultra Pure Grade
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Phenol ≥99% saturated solution in water, Biotechnology Grade, buffered
Supplier: VWR
Packaged at pH 6.6, this mixture is provided with a supplemental alkaline buffer that
allows the researcher to easily increase the pH to 7.9 for DNA purification. When mixed with Chloroform:Isoamyl Alcohol (24:1), it makes an optimized reagent for DNA purification. Doubly distilled, high purity phenol packaged under nitrogen. Safer, easier alternative to crystalline phenol.
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Phenol ≥99.0% saturated with buffer pH 4.5
Supplier: VWR
For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.
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Phenol ≥99% (by anhydrous basis), crystals USP, Macron Fine Chemicals™
Supplier: AVANTOR PERFORMANCE MATERIAL LLC
Crystals.
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Phenol solution, StandARd®, Macron Fine Chemicals™
Supplier: AVANTOR PERFORMANCE MATERIAL LLC
Phenol solution, StandARd®, Macron Fine Chemicals™
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Phenol 99.0-100.5% (by bromometric analysis) Reag. Ph. Eur., ACS, GR for analysis, Supelco®
Supplier: MilliporeSigma
Phenol 99.0-100.5% (by bromometric analysis) Reag. Ph. Eur., ACS, GR for analysis, Supelco®
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Phenol 99.5% unstabilized, loose crystals, extra pure
Supplier: Thermo Scientific Chemicals
Phenol 99.5% unstabilized, loose crystals, extra pure
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Phenol 99%, extra pure
Supplier: Thermo Scientific Chemicals
Phenol 99%, extra pure
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Phenol 99+%, loose crystals for biochemistry
Supplier: Thermo Scientific Chemicals
Phenol 99+%, loose crystals for biochemistry
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Phenol ≥99.5% unstabilized
Supplier: Thermo Scientific Chemicals
Molecular biology
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Phenol ≥99.5% (by GC)
Supplier: TCI America
CAS Number: 108-95-2
Molecular Formula: C6H6O
Molecular Weight: 94.11
Purity/Analysis Method: >99.5% (GC)
Form: Crystal
Color: White
Boiling point (°C): 181
Melting point (°C): 42
Flash Point (°C): 79
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Aquaphenol™, clear, colorless solution
Supplier: MP Biomedicals
Aquaphenol™ is a solution of double-distilled phenol saturated with ultra-pure water (approx 30%). It does not contain antioxidant or additives. Aquaphenol™ has an acid pH and can be used directly for RNA extraction. For DNA extraction, pH can be quickly adjusted to 7,5 or 8 using pH maker solutions and protocols included.
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Phenol 90-100%, Ultrapure
Supplier: MP Biomedicals
Phenol is useful for extraction of viral, plasmid and phage DNAs, and to solubilize and dissociate proteins. It is used as a general disinfectant, either in solution or mixed with slaked lime, etc., for toilets, stables, cesspools, floors, drains, etc.; for the manufacture of colorless or light-colored artificial resins, many medical and industrial organic compounds and dyes; as a reagent in chemical analysis. Aqueous solutions are used as topical anesthetic; topical antiseptic; topical antipruritic. Phenol is used in DNA extractions for removing protein.
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Supplier: Kimberly-Clark
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Supplier: Kimberly-Clark
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Supplier: VWR International