You searched for: Protein Purification

Supplier: Invitrogen

Pierce™ Streptavidin UltraLink™ resin consists of purified recombinant streptavidin that has been covalently immobilized onto a beaded acrylamide support

Supplier: Cytiva

DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

Supplier: Cytiva

Sephadex G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.

Supplier: Cytiva

Blue Sepharose™ 6 Fast Flow is cibacron blue 3G coupled to Sepharose™ 6 Fast Flow. It is well established as an adsorbent for albumin and interferon at both laboratory and process scale. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.

Supplier: Cytiva

MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.

Supplier: Abcam

Streptavidin-HRP Solution (ab210901) is part of the reagents, controls and accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Supplier: Cytiva

Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

Supplier: TCI America

The rGRFT is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Griffithsia sp. This lectin preferably binds to a [Manα(1-2)Man] glycan epitope.
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rGRFT-LecBeads (1 ml gel) shows a binding capacity of about 6 mg or more bovine Ribonuclease B [RNase B] through its binding to a terminal [Manα(1-2)Man] glycan epitope on RNase B.

Supplier: Invitrogen

The Thermo Scientific Pierce™ classic IP kit uses high-quality Protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays with ease.

Supplier: Invitrogen

The Pierce™ HA-Tag Magnetic IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of HA fusion proteins or co-IP experiments using HA-tagged bait proteins.

Supplier: Invitrogen

The Thermo Scientific Pierce Classic Magnetic IP/Co-IP Kit enables highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of antigens and protein-complexes using less than 10 µg of antibody and a magnetic separator.

Supplier: Invitrogen

The Thermo Scientific Pierce Crosslink Magnetic IP/Co-IP Kit uses crosslinking chemistry to covalently immobilize IP antibodies onto premium-quality Protein A/G Magnetic Beads for effective immunoprecipitation and co-immunoprecipitation.

Supplier: Immunoreagents

ImmunoReagents Chicken IgY Affinity Prep resins are prepared using highly purified species specific immunoglobulins

Supplier: Immunoreagents

ImmunoReagents Goat IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins

Supplier: Cytiva

DEAE Sepharose™ CL-6B is a weak anion exchanger.

Supplier: Cytiva

Capto MMC is a multimodal cation exchanger with a different selectivity, compared with traditional ion exchangers.

Supplier: Abcam

Protein G Agarose (High Affinity) (ab193258) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Supplier: Invitrogen

Thermo Scientific Pierce Monomeric Avidin Agarose is ideal for purifying biotinylated proteins, peptides and other molecules.

Supplier: Cytiva

Lentil lectin is a metalloprotein, containing Ca₂₊ and Mn₂₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars.

Supplier: Cytiva

Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.

Supplier: Cytiva

Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.

Supplier: Invitrogen

The kit is designed to simplify the development of a high-yield/high-concentration refolding protocol by using components that have the most significant and general utility in order to limit conditions.

Supplier: Cytiva

Superdex prep grade is a preparative gel filtration medium with a composite matrix of dextran and agarose

Supplier: Cytiva

DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

Supplier: Cytiva

SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.

Supplier: Invitrogen

Thermo Scientific Pierce CDI-Activated Agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilization of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.

Supplier: Cytiva

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.

Supplier: Cytiva

DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.

Supplier: Abcam

Diluent M Concentrate (10X) (ab138908) is part of the reagents, controls and accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Supplier: Invitrogen

TiO2 Phosphopeptide Enrichment Spin Tips, Formulation: 200uL pipette tips containing TiO2 resin; Sufficient For: Processing 96 digested protein samples (0.3-1mg each) containing up to 100ug of phosphopeptides each; 96 tips