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413 results for Protein Purification

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Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

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MabSelect SuRe™ Affinity Chromatography Media, Cytiva

MabSelect SuRe™ Affinity Chromatography Media, Cytiva

Supplier: Cytiva

MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.

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Streptavidin-HRP Solution

Streptavidin-HRP Solution

Supplier: Abcam

Streptavidin-HRP Solution (ab210901) is part of the reagents, controls and accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Epoxy-Activated Sepharose™ 6B Affinity Coupling Media, Cytiva

Supplier: Cytiva

Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

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rGRFT-LecBeads [for Manα(1-2)Man]

Supplier: TCI America

The rGRFT is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Griffithsia sp. This lectin preferably binds to a [Manα(1-2)Man] glycan epitope.
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rGRFT-LecBeads (1 ml gel) shows a binding capacity of about 6 mg or more bovine Ribonuclease B [RNase B] through its binding to a terminal [Manα(1-2)Man] glycan epitope on RNase B.

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Rabbit Affinity Purification Resin, ImmunoReagents

Rabbit Affinity Purification Resin, ImmunoReagents

Supplier: Immunoreagents

ImmunoReagents Rabbit IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins

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Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media, Cytiva

Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.

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Capto™ S ImpAct Ion Exchange Chromatography Media, Cytiva

Capto™ S ImpAct Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

Capto™ S ImpAct is a strong cation exchange chromatography medium (resin) developed for polishing of monoclonal antibodies (MAbs) and other biomolecules.

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MabSelect™ PrismA Protein A Chromatography Resin, Cytiva

MabSelect™ PrismA Protein A Chromatography Resin, Cytiva

Supplier: Cytiva

MabSelect™ PrismA has been improved with an optimised high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing future demands in mAb processing to be met.

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MabSelect Xtra™ Affinity Chromatography Media, Cytiva

MabSelect Xtra™ Affinity Chromatography Media, Cytiva

Supplier: Cytiva

MabSelect Xtra has the same recombinant Protein A ligand as MabSelect but is based on a high-flow agarose base matrix with increased porosity and a slightly decreased particle size compared to MabSelect.

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MabSelect SuRe™ LX Affinity Chromatography Media, Cytiva

MabSelect SuRe™ LX Affinity Chromatography Media, Cytiva

Supplier: Cytiva

MabSelect SuRe LX is a protein A affinity medium with very high dynamic binding capacity at extended residence times and is developed for high titer antibody processes.

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Sepharose™ 6B Gel Filtration Base Matrix

Sepharose™ 6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ 6B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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SP Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

SP Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

SP Sepharose Fast Flow is a strong cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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TMB substrate 1X

TMB substrate 1X

Supplier: Abcam

TMB substrate recommended for use with the Matched Antibody Pair kits for sandwich ELISA.

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Mouse Affinity Purification Resin, ImmunoReagents

Mouse Affinity Purification Resin, ImmunoReagents

Supplier: Immunoreagents

ImmunoReagents Mouse IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins

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Bovine Affinity Purification Resin, ImmunoReagents

Bovine Affinity Purification Resin, ImmunoReagents

Supplier: Immunoreagents

ImmunoReagents Bovine IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins

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SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

SP Sephadex™ C-25 is a strong cation exchanger.

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MabSelect™ VL Protein L Resin, Cytiva

MabSelect™ VL Protein L Resin, Cytiva

Supplier: Cytiva

MabSelect™ VL resin with a redesigned protein L ligand for high binding capacity and improved alkaline stability. For affinity capture of antibody Fabs, bispecific, and other variants containing a kappa light chain.

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SOURCE™ 30Q Ion Exchange Chromatography Media, Cytiva

SOURCE™ 30Q Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

SOURCE™ 30Q is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.

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Superose™ 6 Gel Filtration Media, Prep Grade

Superose™ 6 Gel Filtration Media, Prep Grade

Supplier: Cytiva

Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.

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Q Sepharose™ High Performance Ion Exchange Chromatography Media, Cytiva

Q Sepharose™ High Performance Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.

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Con A Sepharose™ 4B Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method.

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Affinity Purification Kit, T7.Tag, MilliporeSigma

Supplier: MilliporeSigma

T7.Tag Affinity Purification Kit is designed for rapid immunoaffinity purification of target proteins that carry the T7.Tag sequence ( the 11 amino terminal aa of the T7 gene 10 protein).

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EAH Sepharose™ 4B Affinity Coupling Media, Cytiva

EAH Sepharose™ 4B Affinity Coupling Media, Cytiva

Supplier: Cytiva

EAH Sepharose™ 4B allows for the stable carbodiimide-based coupling of carboxyl groups to Sepharose® 4B via an 11-atom hydrophilic spacer arm that permits extremely stable coupling.

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Rabbit IgG Affinity Resin Kit, ImmunoReagents

Supplier: Immunoreagents

For the removal of cross-reactivity to rabbit immunoglobulins.

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CM Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

CM Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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DEAE Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

DEAE Sepharose™ Fast Flow Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

DEAE Sepharose Fast Flow is a weak anion exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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Sepharose™ CL-6B Gel Filtration Base Matrix

Sepharose™ CL-6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ CL-6B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

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Sephadex™ G-15 Gel Filtration Media, Cytiva

Sephadex™ G-15 Gel Filtration Media, Cytiva

Supplier: Cytiva

Sephadex G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.

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NHS-Activated Sepharose™ 4 Fast Flow Affinity Coupling Media, Cytiva

NHS-Activated Sepharose™ 4 Fast Flow Affinity Coupling Media, Cytiva

Supplier: Cytiva

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

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Superose™ 12 Gel Filtration Media, Prep Grade

Superose™ 12 Gel Filtration Media, Prep Grade

Supplier: Cytiva

Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability

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