You searched for: X-Gal (5-Bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

Supplier: VWR

X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) ≥99% (by enzymatic purity), Ultra Pure Grade

Supplier: Thermo Scientific Chemicals

Substrate for beta-galactosidase

Supplier: Thermo Scientific Chemicals

X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) 99%, pure

Supplier: Zymo Research

Sterile, ready to use X-Gal solution.

Supplier: Corning

X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) is a substrate of b-galactosidase, a gene product of the lac operon in E. coli.

Supplier: Thermo Scientific Chemicals

X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) 99+% for molecular biology DNase-, RNase-, protease-free

Supplier: MP Biomedicals

X-Gal, is a Beta-galactosidase substrate, Ultra pure, Purity: >99%, CAS Number: 7240-90-6, Molecular Formula: C14H15BrClNO6, Molecular Weight: 408.6, Synonyms: 5-Bromo-4-chloro-3-indolyl B-D-galactoside, BCIG, Color: White, Form: Powder, Size: 1.0 g

Supplier: MP Biomedicals

Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.

Supplier: MP Biomedicals

5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for b-galactosidase. X-Gal is cleaved by b-galactosidase to yield an insoluble blue precipitate.

Supplier: Enzo Life Sciences

Chromogenic substrate for the enzyme β-galactosidase.

Supplier: TCI America

[for Biochemical Research]
CAS Number: 7240-90-6
MDL Number: MFCD00005666
Molecular Formula: C14H15BrClNO6
Molecular Weight: 408.63
Purity/Analysis Method: >98.0% (HPLC,N)
Form: Crystal
Specific rotation [a]20/D: -66 deg (C=1, DMF)
Storage Temperature: <0°C

Supplier: MilliporeSigma

Convenience for blue/white screening.

Supplier: MilliporeSigma

Alpha-X-Gal Form White to off-white solid Cas number 1174456-59-7 Purity =98% by TLC Storage -20C A chromogenic substrate for Alpha -galactosidase. Useful for histochemical studies.

Supplier: Teknova

Used in conjunction with cloning systems that use b-galactosidase activity as an indicator of nonrecombination.

Supplier: Ambeed

X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) 98%

Supplier: Promega Corporation

X-Gal, in conjunction with IPTG, is used to detect β-galactosidase activity to differentiate recombinants from non-recombinants in cloning experiments using vectors containing the lacZ or lacZ α-peptide gene.

Supplier: MERIDIAN LIFE SCICNE, INC BE

X-Gal is a chromogenic substrate which forms an intense blue precipitate that is used to detect the presence of the gal gene and beta-galactosidase enzyme activity.

Supplier: Biotium

is the most widely used chromogenic substrate for b-galactosidase. Enzyme hydrolysis of the substrate yields a dark blue colored precipitate (max ~ 615nm).

Supplier: IBI Scientific

X-GAL is a histochemical substrate for β-galactosidase, allowing the detection of lac gene expression

Supplier: Matrix Scientific

Matrix Scientific Part Number: 008823-1G , MDL Number: MFCD00005666

Supplier: Adipogen

Molecular biology reagent. Chromogenic substrate for beta-galactosidase. Used in gene cloning together with IPTG (Prod. No. AG-CC1-0002) to indicate whether a cell expresses the beta-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening. Distinguishes between recombinant and non-recombinant plasmids carrying the beta-galactosidase gene. Used in two-hybrid analysis. X-Gal is used to determine E. coli and coliform content in drinking water samples.

Supplier: OZ Biosciences

X-Gal substrate is metabolized by the β-galactosidase enzyme into an insoluble blue precipitate. It is ideal for staining transformed bacteria and LacZ transfected or infected cells, tissues and organisms.