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You searched for: Protein Assays

Protein Assays

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Pierce™ Phosphoprotein Phosphate Estimation Kit, Thermo Scientific

Pierce™ Phosphoprotein Phosphate Estimation Kit, Thermo Scientific

Supplier: Invitrogen

Pierce™ Phosphoprotein Phosphate Estimation Assay Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level.

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Pierce™ Firefly Luciferase Flash Assay Kit, Thermo Scientific

Pierce™ Firefly Luciferase Flash Assay Kit, Thermo Scientific

Supplier: Invitrogen

The Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

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Pierce™ Protein Conjugate, Horseradish Peroxidase, Biotinylated, Thermo Scientific

Pierce™ Protein Conjugate, Horseradish Peroxidase, Biotinylated, Thermo Scientific

Supplier: Invitrogen

Biotinylated horse radish peroxidase is used as control or signal amplification in IHC to amplify the signal of biotinylated primary antibodies using the avidin-biotin complex staining method.

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Pierce™ Firefly Luciferase Glow Assay Kit, Thermo Scientific

Pierce™ Firefly Luciferase Glow Assay Kit, Thermo Scientific

Supplier: Invitrogen

The Pierce™ Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays.

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Pierce EZ™ Biotin Quantitation Kit, Thermo Scientific

Pierce EZ™ Biotin Quantitation Kit, Thermo Scientific

Supplier: Invitrogen

Pierce™ Biotin Quantitation Kit includes aliquots of HABA reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.

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Pierce ProFound™ Co-Immunoprecipitation Kits, Thermo Scientific

Supplier: Invitrogen

An ideal system for designing, constructing, and performing a controlled co-immunoprecipitation (co-IP) experiment.

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RhoA/Rac1/CDC42 Activation Assay Combo Kit, Cell Biolabs

RhoA/Rac1/CDC42 Activation Assay Combo Kit, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' RhoA/Rac1/Cdc42 Activation Assay Combo Kit uses visible agarose beads to selectively precipitate the active form of Rho, Rac and Cdc42

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Octet® Protein A (ProA) Biosensor, Sartorius

Supplier: SARTORIUS CORPORATION MS

For determination of antibody concentration.

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96-Well Plates for Pierce™ Quantitative Fluorometric Peptide Assay, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific™ 96-Well Plates are manufactured using high-quality polystyrene and are high-binding with black, flat well bottoms to accommodate fluorescence assays such as the Pierce™ Quantitative Fluorometric Peptide Assay.

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Anti-Rabbit Detection Module, Protein Simple

Supplier: 0000014126

Use with a Jess/Wes or Sally Sue/Peggy Sue Separation Module.

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Reagents for Pierce PeroXOquant™ Peroxidase Assays, Thermo Scientific

Reagents for Pierce PeroXOquant™ Peroxidase Assays, Thermo Scientific

Supplier: Invitrogen

Reagents for Pierce PeroXOquant™ Peroxidase Assays, Thermo Scientific

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Pierce ProFound™ Biotinylated-Protein Interaction Pull Down Kit, Thermo Scientific

Pierce ProFound™ Biotinylated-Protein Interaction Pull Down Kit, Thermo Scientific

Supplier: Invitrogen

Pierce™ Biotinylated Protein Interaction Pull-Down Kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.

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ROS Detection Assay Kit, OZ Biosciences

ROS Detection Assay Kit, OZ Biosciences

Supplier: OZ Biosciences

The ROS Detection Assay kit (Green Fluorescence) is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2’, 7’-Dichlorodihydrofluorescein diacetate (DCF-DA).

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Pierce PeroXOquant™ Quantitative Peroxide Assay Kits (Lipid), Thermo Scientific

Pierce PeroXOquant™ Quantitative Peroxide Assay Kits (Lipid), Thermo Scientific

Supplier: Invitrogen

Pierce™ Quantitative Peroxide Assay Kit (Lipid) detects and measures hydrogen peroxide levels in biological samples using an Iron and Xylenol orange* (XO) reagent.

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SARS-CoV-2 Spike RBD Titer Assay Kit, Acro Biosystems

Supplier: ACROBIOSYSTEMS

This kit is developed for detecting SARS-CoV-2 Spike RBD in the sample.

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OxiSelect™ TBARS Assay Kit (MDA Quantitation), Cell Biolabs

OxiSelect™ TBARS Assay Kit (MDA Quantitation), Cell Biolabs

Supplier: Cell Biolabs

OxiSelect™ TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct

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MELISA-TEK™ Meat Species Kits, Elisa Technologies Inc

Supplier: ELISA TECHNOLOGIES MS

The MELISA-TEK™ Meat Species Kit detects animal species proteins in meat and bone meals and animal feeds.

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Pierce™ Protein Conjugate, Bovine Serum Albumin, Biotinylated, Thermo Scientific

Pierce™ Protein Conjugate, Bovine Serum Albumin, Biotinylated, Thermo Scientific

Supplier: Invitrogen

Biotinylated bovine serum albumin (B-BSA) is used as a positive control in biotin quantitation methods.

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Pierce™ Marfey's Reagent, Thermo Scientific

Pierce™ Marfey's Reagent, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce Marfey's Reagent (FDAA) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.

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Rap Activation Assay Kits, Cell Biolabs

Rap Activation Assay Kits, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap

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His Capture Kit, Type 2, Cytiva

Supplier: Cytiva

Reagents for capture of histidine-tagged molecules as ligands in biomolecular interaction analysis. Designed for use with Biacore 8K+, Biacore 8K (larger kit component volumes than in His Capture Kit) but can be used with all other Biacore systems.

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Pierce™ Quantitative Fluorometric Peptide Assay, Thermo Scientific

Supplier: Invitrogen

The Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay is a sensitive, mix-and-read fluorescent microplate assay for the quantitative measurement of peptides and peptide mixtures.Sensitive—accurately detect as little as 5.0 µg/mL of single peptides or peptide mixturesRobust— assay performance rigorously tested using both peptides and peptide digest mixturesRobust peptide digest standard—kit includes a validated peptide digest standard for improved reproducibility of quantitationCompatible—works with many reagents, including those used in mass spectrometry sample preparationConvenient—easy mix-and-read format and stable fluorescent signal that may be read in as little as 5 minutes up to several hoursThe Pierce Quantitative Fluorescent Peptide Assay reagents include peptide assay buffer, fluorescent peptide labeling reagent, and a peptide digest assay standard for the quantitative measurement of peptide concentrations

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NTA Reagent Kit, Cytiva

NTA Reagent Kit, Cytiva

Supplier: Cytiva

Reagents for sensor chip NTA, used for His capture in His-tagged molecules in biomolecular interaction analysis.

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pMCS Tluc16-DD Vector for Luciferase Assays, Thermo Scientific

Supplier: Invitrogen

The Thermo Scientific™ pMCS Tluc16-DD Vector is a multiple cloning site (MCS) vector designed to accept a promoter sequence for study of gene regulation using the intracellular TurboLuc™16 (Tluc16) luciferase reporter.Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systemsMultiple unique cloning sites provide versatility for transfer of regulatory elements from one vector to anotherDual-destabilization (DD) technology reduces accumulation of Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assaySynthetic polyA terminator and Transcriptional Pause Site (TPS) included upstream of MCS to minimize non-specific transcriptional read-throughTluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family

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pEF1α Tluc16-DD Vector for Luciferase Assays, Thermo Scientific

Supplier: Invitrogen

The Thermo Scientific™ pEF1α Tluc16 Vector encodes the smallest known luciferase, TurboLuc™16 (Tluc16) luciferase, as a reporter under a modified version of the Elongated Factor 1α (EF1α) promoter for constitutive Tluc16 luciferase expression in mammalian cells.Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systemsTruncated EF1α promoter for constitutive Tluc16 luciferase expressionTluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family

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