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Protein Assays

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

VWR® Bradford Method Protein Assay Kit

VWR® Bradford Method Protein Assay Kit

Supplier: VWR

The Bradford Protein Assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method. The standard protocol is used for measuring 10-100 μg of protein and the microassay is used for measuring 1-10 μg of protein. For maximum convenience, Bradford Reagent and 0.5 mg/ml BSA standard solution are offered individually or as components of a kit.

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PROTEOSTAT® Protein Aggregation Assay, Enzo Life Sciences

PROTEOSTAT® Protein Aggregation Assay, Enzo Life Sciences

Supplier: Enzo Life Sciences

PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution

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CB™ Protein Assay, G-Biosciences

CB™ Protein Assay, G-Biosciences

Supplier: G-Biosciences

It is an improved Coomassie Dye based protein assay based on the Bradford Protein Assay

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CB-X™ Protein Assay, G-Biosciences

CB-X™ Protein Assay, G-Biosciences

Supplier: G-Biosciences

A major problem for researchers is to select a protein assay from the vast selection on the market that is compatible with their protein sample

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RED 660™ Protein Assay, G-Biosciences

RED 660™ Protein Assay, G-Biosciences

Supplier: G-Biosciences

RED 660™ protein assay is a single reagent colorimetric assay that outperforms commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays

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Bicinchoninic Acid (BCA) Protein Assay, G-Biosciences

Bicinchoninic Acid (BCA) Protein Assay, G-Biosciences

Supplier: G-Biosciences

The Bicinchoninic Acid (BCA) protein assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions

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NI™ (Non-Interfering™) Protein Assay, G-Biosciences

NI™ (Non-Interfering™) Protein Assay, G-Biosciences

Supplier: G-Biosciences

A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation

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Protein dotMETRIC™ Assay, G-Biosciences

Protein dotMETRIC™ Assay, G-Biosciences

Supplier: G-Biosciences

Rapidly mix 1µL protein sample with the supplied dilution buffer and apply 1µL of the solution to the test strip by point-of-contact capillary action

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BCA Protein Assay Kit, Thermo Scientific

Supplier: Thermo Scientific Chemicals

BCA Protein Assay Kit can be used to measure very dilute protein concentrations in very small sample volumes.

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QuantiFluo™ Protein Assay Kit, BioAssay Systems

QuantiFluo™ Protein Assay Kit, BioAssay Systems

Supplier: BIOASSAY SYSTEMS

For quantitative fluorimetric determination of total protein.

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Total Protein Assay Kit, BioAssay Systems

Total Protein Assay Kit, BioAssay Systems

Supplier: BIOASSAY SYSTEMS

For quantitative determination of total protein

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QuantiChrom™ Protein Assay Kit, BioAssay Systems

QuantiChrom™ Protein Assay Kit, BioAssay Systems

Supplier: BIOASSAY SYSTEMS

For quantitative determination of total protein (Bradford method).

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AzureRed Fluorescent Protein Stain, Azure BioSystems

AzureRed Fluorescent Protein Stain, Azure BioSystems

Supplier: Azure Biosystems

AzureRed Fluorescent Protein Stain is a quantitative total protein stain for blots and blots with the sensitivity to detect less than 1 ng of protein per band or spot

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Protn-Latex™, G-Biosciences

Protn-Latex™, G-Biosciences

Supplier: G-Biosciences

A simple and reliable method for estimation of protein contamination in latex

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Protein Carbonyl Colorimetric Assay Kit, Cayman Chemical Company

Protein Carbonyl Colorimetric Assay Kit, Cayman Chemical Company

Supplier: Cayman Chemical Company

Convenient colorimetric assay for measurement of oxidized proteins.

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SUMO-QAPTURE-T® Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences

The only commercially available kit for isolation and enrichment of SUMOylated proteins.

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PROTEOSTAT® Protein Refolding and Aggregation Sensing Kit, Enzo Life Sciences

PROTEOSTAT® Protein Refolding and Aggregation Sensing Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences

ProteoStat® Protein refolding and aggregation sensing kit provides a fractional factorial matrix design to facilitate screening of refolding parameters for a specific protein in different buffers, and a simple, homogenous assay format for monitoring protein aggregation using a red-emitting aggregation-sensitive dye. 

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eFluxx-ID® Multidrug Resistance Assay Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences

The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP

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Pierce™ BCA® Protein Assay Kits and Reagents, Thermo Scientific

Pierce™ BCA® Protein Assay Kits and Reagents, Thermo Scientific

Supplier: Invitrogen

Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

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Pierce™ Micro BCA™ Protein Assay Kit and Reagents, Thermo Scientific

Pierce™ Micro BCA™ Protein Assay Kit and Reagents, Thermo Scientific

Supplier: Invitrogen

The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples.

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DC™ Protein Assay, Bio-Rad

DC™ Protein Assay, Bio-Rad

Supplier: Bio-Rad

The DC™ (detergent compatible) protein assay is a colorimetric assay for protein concentration following detergent solubilization

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Pierce™ Coomassie (Bradford) Protein Assay Kits, Thermo Scientific

Pierce™ Coomassie (Bradford) Protein Assay Kits, Thermo Scientific

Supplier: Invitrogen

The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.

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Bio-Rad® Protein Assays, Bio-Rad

Supplier: Bio-Rad

The Bio-Rad® Protein Assay is a simple colorimetric assay for measuring total protein concentration, based on the Bradford dye-binding method

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Bradford Protein Assay Kit (B-Pak), OZ Biosciences

Bradford Protein Assay Kit (B-Pak), OZ Biosciences

Supplier: OZ Biosciences

Bradford Protein Assay Kit (B-Pak) is a straightforward and rapid kit for determining the concentration of proteins in solution.This ready-to-use kit is provided with 1X reagent & 2 sets of BSA standards which means that no dilution, filtration or calculation are required

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10x Ubiquitinylation Buffer, Enzo Life Sciences

Supplier: Enzo Life Sciences

Assay Buffer from the Ubiquitinylation Kits

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Proteasome 20S Assay Kit, Enzo Life Sciences

Proteasome 20S Assay Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences

A QuantiZyme™ Assay System

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PROTEOSTAT® Protein Aggregation Standards, Enzo Life Sciences

PROTEOSTAT® Protein Aggregation Standards, Enzo Life Sciences

Supplier: Enzo Life Sciences

This kit is ideal for reliable and accurate quantification of protein aggregation in solution.

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Caspase-2 Fluorometric Assay Kit, Enzo Life Sciences

Caspase-2 Fluorometric Assay Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences

Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VDVAD-AFC.

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Pierce™ Coomassie Plus™ (Bradford) Protein Assay Kit, Thermo Scientific

Pierce™ Coomassie Plus™ (Bradford) Protein Assay Kit, Thermo Scientific

Supplier: Invitrogen

The Pierce™ Coomassie Plus™ Protein Assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.

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Pierce™ Modified Lowry Protein Assay Kit, Thermo Scientific

Pierce™ Modified Lowry Protein Assay Kit, Thermo Scientific

Supplier: Invitrogen

The Modified Lowry Protein Assay Kit is the preferred colorimetric assay for quantifying total protein concentration.

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