You searched for: Enzyme Assays

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay System

Supplier: Enzo Life Sciences

The PTP1B Tyrosine Phosphatase Drug Discovery Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.

Supplier: Enzo Life Sciences

A Fluor de Lys® fluorescent assay system

Supplier: Enzo Life Sciences

Uses:

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay System

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay System

Supplier: Enzo Life Sciences

A QuantizymeTM Assay System

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay System

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay System

Supplier: Enzo Life Sciences

This kit provides a simple, homogenous assay for screening modulators of Thioredoxin-1 enzymatic activity.

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay System

Supplier: Enzo Life Sciences

An improved Fluor de Lys® HDAC assay with Fluor de Lys®-Green, a new substrate offering higher sensitivity and an excitation and emission (485/530nm) that avoids quenching and fluorescent interference from compounds absorbing in the near UV and blue range.The Fluor de Lys®-Green HDAC assay is a complete kit for measuring histone deacetylase (HDAC) activity in cell or nuclear extracts, immunoprecipitates or purified enzymes

Supplier: Enzo Life Sciences

The LSD1 Fluorimetric Drug Discovery Kit provides human recombinant LSD1 and all reagents for measuring its activity in a sensitive, real-time fluorescent assay.

Supplier: Enzo Life Sciences

The DPPIV Drug Discovery Kit is a complete assay system designed to screen DPPIV inhibitors, a potential therapeutic target, providing enough material to perform at least 96 assays.

Supplier: Enzo Life Sciences

For the measurement of glutathione peroxidase in cell lysates, plasma, and tissue from any species.

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay SystemThe MMP-9 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-9 (MMP-9, gelatinase B) using a quenched fluorogenic peptide: OmniMMPTM fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH

Supplier: Enzo Life Sciences

A Fluor de Lys® fluorescent assay system

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy.

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the cathepsin K LeuArg peptide recognition sequence, this kit allows the detection of cathepsin K activity in living cells by fluorescence microscopy.

Supplier: Molecular Devices

IMAP® technology provides a homogeneous assay applicable to a wide variety of kinases without regard for substrate peptide sequence.

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay SystemThe MMP-7 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-7 (MMP-7, matrilysin) using a thiopeptide as a chromogenic substrate.

Supplier: Enzo Life Sciences

The Granzyme B Assay Kit for Drug Discovery is a complete assay system designed to measure the protease activity of granzyme B. It contains a colorimetric substrate (IEPD-pNA) to measure this activity; cleavage of the p-nitroanilide (pNA) group from the colorimetric substrate increases absorption at 405 nm.

Supplier: Enzo Life Sciences

Novel Cell-free Sclerostin/LRP Binding Assay Ideal for Primary Inhibitor Screens.

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the cathepsin L PheArg peptide recognition sequence, this kit allows the detection of cathepsin L activity in living cells by fluorescence microscopy.

Supplier: Enzo Life Sciences

The MMP inhibitor profiling kit, fluorometric (also known as fluorimetric) RED is a complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes, using a quenched fluorogenic substrate OMNIMMP® RED: TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6’-TAMRA)-Ala-Lys-NH2 [TQ3=quencher; GABA=4-aminobutyric acid; Cha=L-cyclohexylalanine; Abu=2-aminobutyric acid; Smc=S-methyl-L-cysteine; Dab=2,4-diaminobutyric acid; 6’-TAMRA=6’-tetramethylrhodamine]. TAMRA fluorescence is thoroughly quenched by the TQ3 group until cleavage by MMPs separates the two moieties.
The assays are performed in a convenient 96-well microplate format. The compound NNGH is also included as a prototypic control inhibitor.

Supplier: Enzo Life Sciences

The AEC Peroxidase Substrate Kit contains reagents for visualization of peroxidase reactions using Aminoethylcarbazole (AEC). In the presence of H2O2, peroxidases catalyze the oxidation of AEC into a brick red precipitate. The supplied reagents are sufficient for preparation of 300 ml of ready-to-use color reaction mixture.

Supplier: Enzo Life Sciences

It is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays. Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control.

Supplier: Enzo Life Sciences

BIOMOL Green™ provides a simple and convenient method for colorimetric phosphate quantitation (abs. 600-680 nm). Unlike other molybdate/malachite green-based assays, BIOMOL Green™ doesn’t require multiple solutions or reagents prepared fresh on the day of the assay. BIOMOL Green™ is extremely stable (>6 mos. at 4°C) and is simply mixed, at room temperature, with any enzymatic reaction that has released free phosphate. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.BIOMOL Green™ has perhaps been most widely applied to protein phosphatase assays. Typically, a protein phosphatase (e.g. PTP1B or LAR) is simply incubated with an appropriate phosphopeptide (e.g. IR5,9,10). Addition of BIOMOL Green™ stops the reaction and begins color development, which is read 20-30 min. later.BIOMOL Green™ may be used in cuvette or microplate-based assays and is ideal for high-throughput applications. Each kit comes with a standardized phosphate solution for assay calibration.

Supplier: MilliporeSigma

Native phospholipase D from Streptomyces chromofuscus