You searched for: Proteins and Peptides

Supplier: Anaspec Inc

The fibrinopeptide B is an N-terminal modified peptide with pyroglutamylation, one of the common post-translational process. After cleaving off FPA in the conversion of soluble fibrinogen to fibrin, thrombin then releases fibrinopeptide B (FPB) from the beta-chains of the fibrin I subunits to form fibrin II polymers, which associate and cross-links to form a semi-solid network, while the fibrinopeptides remain soluble in plasma. Fibrin formation associated with active thrombosis leads to significantly higher plasma levels of FPB and thus measurement of plasma FPB levels is a more sensitive and specific serologic marker for acute thrombosis.
Sequence:Pyr-GVNDNEEGFFSAR
MW:1553.6 Da
%Peak area by HPLC:≥95%
Storage condition: -20°C

Supplier: ADVANCED BIOMATRIX, INC. MS

PhotoCol®-LAP is a methacrylated Type I collagen kit.These 3D collagen gels can be prepared at various concentrations and photocrosslinked (405nm) to provide various gel stiffness. Each kit contains 100 mg of methacrylated collagen, LAP photoinitiator, acetic acid and a neutralization solution.

Supplier: Anaspec Inc

Protein A-HiLyte™ Fluor 750 Conjugate can be used as a universal reagent to detect primary antibodies in Western Immunoblot from many species including rabbit, human, some mouse IgG isotypes, and others. Fluorescence (near-infrared) Excitation/Emission wavelength: 754 nm/778 nm.
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed using a recombinant bacterial expression system. Its apparent molecular weight is approximately 30,000 Da compared to 42,000 Da for the native Protein A that is found in Staphylococcus aureus.
Protein A conjugates are widely used as labeled reagents for a variety of experiments including immunoprecipitation, antibody detection and purification and assay development

Supplier: Adipogen

Clusterin shares homology with the small heat shock protein family of molecular chaperones. The mature secreted form of the protein is a glycosylated, 80-kDa disulfide-linked heterodimer of alpha and beta subunits (produced by internal cleavage). Clusterin is expressed in virtually all tissues and found in all human fluids. It is involved in numerous physiological processes important for carcinogenesis and tumor growth, including apoptotic cell death, cell cycle regulation, DNA repair, cell adhesion, tissue remodeling, lipid transportation, membrane recycling, and immune system regulation. Clusterin also exists as a nuclear protein. The secreted form of Clusterin has extracellular chaperone and anti-apoptotic activities while the nuclear form acts as a proapoptotic factor.

Supplier: Adipogen

Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V competes for phosphatidylserine binding sites (e.g. platelets) with prothrombin or coagulation factors and inhibis the activity of phospholipase A1. Antibodies directed against annexin V are found in patients with a disease called the antiphospholipid syndrome(APS), a thrombophilic disease associated with autoantibodies against phospholipid compounds. Annexin V is used as a probe in the "annexin V affinity assay" to detect cells that have expressed phosphatidylserine on the cell surface, a feature found in apoptosis and other forms of cell death.

Supplier: Anaspec Inc

This glycopeptide is a 16-amino acid modified fragment of mucin 5/MUC5AC, where T* is a GalNac labeled threonine 3. Mucin-type linkages (GalNAc 1-O-Ser/Thr) are initiated by a family of glycosyltransferases known as the UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferases (ppGaNTases). These enzymes transfer GalNAc from the sugar donor UDP-GalNAc to serine and threonine residues, forming an alpha anomeric linkage. The MUC5AC gene, which is mainly expressed in gastric and respiratory mucosae, exhibits 8 amino acid tandemly repeated domain, the consensus peptide TTSTTSAP.
Sequence:GT-T*-PSPVPTTSTTSAP (* = GalNAc-modified residue)
MW:1703.9 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec Inc

A hallmark of Alzheimer's disease (AD) is the presence of beta-Amyloid in plaques in the brains of patients. N-terminally truncated pyroglutamate-modified beta-Amyloid forms such as Aß(3-40) and Aß (11- 40) have been described as major compounds in the senile plaques. Pyro-Glu modified beta-Amyloid forms are more resistant to degradation, show higher toxicity and have increased aggregation propensity compared to non-modified beta-Amyloid.
Sequence: Pyr-VHHQKLVFFAEDVGSNKGAIIGLMVGGVV
Molecular Weight: 3133.7 Da
% Peak Area by HPLC: ≥95
Peptide Content: ≥ 60%
Storage condition: -20°C

Supplier: Adipogen

GSH-OEt is cellpermeable and has been used to protect cells against radiation damage, oxidants and various toxic compounds including heavy metals. GSH-OEt is a protective agent against cellular damage, such as cataracts and mitochondrial degeneration.

Supplier: Adipogen

OX40L is a membrane-expressed cytokine that belongs to the TNF ligand family. It acts as costimulator through interaction with its ligand OX40 on T cells, stimulating T cell activation, proliferation and cytokine production. OX40L is expressed on antigen presenting cells including B cells, dendritic cells, mast cells and endothelium. MultimericOX40L is a high activity construct in which two trimeric OX40L ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively activates T cell proliferation.

Supplier: Adipogen

CCL22 (C-C Motif Chemokine 22; Stimulated T cell chemotactic protein; STCP1) is a CC chemokine initially isolated from clones of monocyte-derived macrophages and binds to the receptor CCR4. Human CCL22 is highly expressed in macrophage and in monocyte-derived dendritic cells and thymus. It is also found in lymph node, appendix, activated monocytes, resting and activated macrophages. May play a role in the trafficking of activated/effector T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in dendritic cells, IL-2 activated NK cells and activated T lymphocytes.

Supplier: Adipogen

Interleukin 6 (IL-6) is a pleiotropic alpha-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22kDa-28kDa phosphorylated and variably glycosylated molecule. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6R) and a signal transducing subunit (gp130). IL-6 is a key factor for the growth of plasma cells.

Supplier: Adipogen

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac1. CD54 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1beta and LPS. Soluble CD54 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

Supplier: Anaspec Inc

This is Oxytocin (OT) N-terminally labeled with Biotin. OT is a 9-amino acid peptide that is synthesized primarily in the hypothalamic neurons, specifically the magnocellular oxytocin neurons in the supraoptic and paraventricular nuclei and is secreted by the posterior pituitary gland. It is also secreted from other tissues, such as the ovaries and testes. Circulating OT is important during parturition and lactation. In the pregnant uterus, oxytocin and the oxytocin receptor play a major part for uterine contractility and the induction of labor. OT is also involved in complex social behaviors such as affiliation, sexual behavior, social recognition, stress buffering, aggression, and trust.
Sequence: Biotin-CYIQNCPLG-NH2 (Disulfide bridge: 1-6)
MW: 1234.5 Da
% Peak area by HPLC: 95
Storage condition: -20° C

Supplier: Anaspec Inc

Group II of distinct 18 peptide mixtures, in combination with Group I of 180 distinct peptide mixtures (cat# 62017-1 or cat# 62017-5), obtained via positional scanning synthetic peptide combinatorial library (PS-SPCL) can provide sequence preference information of different kinases. Amount provided is 1 mg of peptide mixture x 18 vials. Sequences: Y-A-pT-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-pT-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-pT-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-X-pT-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-X-X-pT-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-X-X-X-S/T-pT-X-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-X-X-X-S/T-X-pT-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-X-X-X-S/T-X-X-pT-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-X-X-X-S/T-X-X-X-pT-A-G-K-K(LC-biotin)-NH2 Y-A-pY-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-pY-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-pY-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-X-pY-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-X-X-pY-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-X-X-X-S/T-pY-X-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-X-X-X-S/T-X-pY-X-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-X-X-X-S/T-X-X-pY-X-A-G-K-K(LC-biotin)-NH2 Y-A-X-X-X-X-X-S/T-X-X-X-pY-A-G-K-K(LC-biotin)-NH2 X = degenerate mixture of the 17 natural amino acids excluding cysteine, serine, and threonine. LC = 'long chain' version with an additional aminohexanoic acid spacer between the biotin and lysine side chain. S/T = equimolar mixture of serine and threonine. pT is phosphothreonine, and pY is phosphotyrosine.

Supplier: Adipogen

CD279 (Programmed Cell Death Protein 1; PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses. Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA-4 and PD-1). CD279 is expressed on activated T cells, B cells, myeloid cells and on a subset of thymocytes. In vitro, ligation of CD279 inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10 and IFN-gamma. In addition, CD279 ligation also inhibits BCR mediated signaling. CD279 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases.

Supplier: Adipogen

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Supplier: Adipogen

EGFR has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 in the absence of a co-receptor. Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGFR signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGFR signaling has also been shown to play a role in carcinogenesis.

Supplier: Adipogen

Wnts are lipid-modified morphogens that control many processes during development, stem cell maintenance and homeostasis and their aberrant regulation has been linked to diseases in man including diabetes, neurodegeneration and cancer. Three distinct receptor families mediate Wnt signaling: Fzs, the single-pass LRP5/6 co-receptors and the atypical tyrosine kinase receptors Ror2 and Ryk. Frizzled proteins can activate canonical Wnt/beta-catenin signaling as well as non-canonical pathways (Wnt/Ca2+ pathways and planar cell polarity). The cysteine-rich domain (CRD) of the frizzled receptors situated on the extracellular part of the receptor is sufficient to bind the Wnt ligands.

Supplier: STEMCELL Technologies

Growth differentiation factor 5 (GDF-5) is a member of the bone morphogenetic protein (BMP) subclass of the transforming growth factor beta (TGF-β) superfamily. It binds a receptor complex comprising BMPR1B and BMPR2, which then activates the SMAD signaling pathway (Carreira et al.; Nishitoh et al.; Osório et al.). GDF-5 enhances chondrogenic differentiation of mesenchymal stem cells, skeletogenesis, and dendrite growth during development (Coleman et al.; Francis-West et al.). Studies in rat demonstrated that GDF-5 regulates patterning, neurogenesis, astrogliogenesis, and neuronal specification (Gajavelli et al.; Krieglstein et al.; O’Keeffe et al.).

Supplier: Adipogen

Mouse CD83 is a 30-35kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins. CD83 is a primary marker for dendritic cells. It is also found on B cells, neutrophils, monocytes and macrophages. Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on target cells. Membrane CD83 promotes T cell proliferation, particularly of CD8+ cytotoxic T cells. Soluble CD83 is immunosuppressive and blocks T cell activation. On monocytes, CD83 is suggested to drive monocytes into a fibrocyte phenotype. A lack of membrane-expressed CD83 leads to an unusual IL-4/ IL-10 producing CD4+ T cell phenotype.

Supplier: Adipogen

The LTbetaR activates two different NF-kappaB pathways that lead to distinct patterns of gene induction, including selected chemokines, and the cytokine BAFF, which is essential for the survival of mature B lymphocytes. LTbetaR activates the classical NF-kappaB (relA/p50) pathway, like the type 1 TNF receptor (TNFR1), that regulates proinflammatory genes, like the chemokine MIP1beta. However, LTbetaR, unlike TNFR1, also activates the processing of p100 to form RelB/p52 complexes, which activate genes involved in lymphoid organ formation and lymphocyte survival.

Supplier: STEMCELL Technologies

Epidermal growth factor (EGF) is the founding member of the EGF family of proteins and is characterized by high affinity binding to various EGF receptors (EGFRs) and the production of mitogenic responses (Carpenter and Cohen). EGF promotes EGFR dimerization, resulting in activation of downstream pathways including PI3K, ERK1/2, JAK/STAT, β-catenin, and calcium signaling. EGF is secreted by the gut-associated salivary and Brunner’s glands, is found in a variety of body fluids, and stimulates cell proliferation and differentiation in rodent and neonatal human intestine (Wright et al.). EGF can stimulate the proliferation of mouse embryonic stem cells or induce the terminal differentiation/growth inhibition of A431 cells (Heo et al.).

Supplier: Anaspec Inc

This GLP-1 (7-36) amide, is labeled with biotin at the peptide N-terminus. GLP-1 (7-36) amide is an incretin hormone that causes glucose dependent release of insulin by pancreatic beta cells. It is the cleavage product of GLP-1 (1-36) amide peptide (Cat# AS-22460). Both GLP-1 (7-36) and GLP-1 (7-37)-Cat# AS-20761, also play roles in gastric motility (gastric emptying), on the suppression of plasma glucagon levels (glucose production) and possibly on the promotion of satiety and stimulation of glucose disposal in peripheral tissues independent of the actions of insulin. GLP-1 (7-36) has a short half life of less than 2 minutes, and like GIP, is rapidly degraded by the enzyme dipeptidyl peptidase IV (DPP-4), which is widely expressed in a number of sites, including the endothelial cells of small gut arterioles. DPP-4 degrades GLP-1 (7-36) into the non insulinotropic GLP-1 (9-36)-Cat# AS-65070 (some studies suggest it may have weak insulinotropic activity). As a result, the majority of GLP-1 (and GIP) is inactivated as an insulinotrope before reaching the systemic circulation.
Sequence: Biotin-HAEGTFTSDVSSYLEGQAAKEFIAWLVKGR-NH2
MW: 3524 Da
% Peak area by HPLC: 95
Storage condition: -20° C

Supplier: STEMCELL Technologies

SARS-CoV-2 Recombinant Spike Protein, aa16-685 is expressed in HEK293 cells and is one of four structural proteins encoded by the SARS-CoV-2 genome. The Spike Protein plays a key role in attachment to host cells, allowing invasion through clathrin-mediated endocytosis. The Spike Protein can be cleaved by host cell proteases after aa685 to yield the N-terminal S1 subunit and C-terminal S2 region. The S1 subunit is responsible for interacting with the host cell receptor (angiotensin-converting enzyme II) through a receptor-binding domain that is highly conserved with SARS-CoV. The S1 subunit has two conformations: a ‘down’ conformation in which the receptor is inaccessible, and an ‘up’ conformation in which the receptor is accessible. These conformational changes are key for monoclonal antibody drugs and vaccine development. SARS-CoV-2 Recombinant Spike Protein contains a polyhistidine tag at the amino terminus; it also contains a FLAG tag at the carboxy terminus.

Supplier: BioVendor

Cysteine-rich secretory protein 3 (CRISP-3, SGP 28) belongs to the CRISP family of proteins having a PR-1 (pathogenesis related protein 1) domain at its N-terminal and ion channel regulatory (ICR) domain at its C-terminal connected by a hinge region. CRISP-3 transcripts are shown to be present predominantly in the prostate. Its expression is low in benign prostatic epithelium but is highly upregulated in the majority of high-grade prostatic intraepithelial neoplasia lesions and in most primary prostate tumours and metastases. Two independent studies have shown that CRISP-3 is one of the genes that are most often upregulated in prostate cancer. Human Prostate Secretory Protein (PSP94) has been shown to bind human CRISP-3 with very high affinity. Functional significance of this complex is not yet known. Yet, it was suggested, that involvement of the hinge region of CRISPs in interaction with PSP94 may affect the domain movement of CRISPs essential for the ion-channel regulatory activity resulting in inhibition of this activity.

Supplier: Adipogen

Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells, and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils, and induces mononuclear cells to secrete IFN-gamma and TNF-alpha and -beta. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important hemeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.

Supplier: BioVendor

Cystatin C is a non-glycosilated basic single-chain protein consisting of 120 amino acids with a molecular weight of 13.36 kDa and is characterized by two disulfide bonds in the carboxy-terminal region. It belongs to the cystatins superfamilly which inactivates lysosomal cysteine proteinases, e.g. cathepsin B, H,.K, L and S. Imbalance between Cystatin C and cysteine proteinases is associated with inflammation, renal failure, cancer, Alzheimer's disease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Its increased level has been found in patients with autoimune diseases, with colorectal tumors and in patients on dyalisis. Serum Cystatin C seems to be better marker of glomerular filtration rate than creatinine. On the other hand, low concentration of Cystatin C presents a risk factor for secondary cardiovascular events.

Supplier: Adipogen

IL-36alpha (IL-1F6), IL-36beta (IL-1F8) and IL-36gamma (IL-1F9) bind to IL-36R (IL-1Rrp2) and IL-1RAcP, activating similar intracellular signals as IL-1. IL-36Ra inhibits the production of proinflammatory cytokines, including IL-12, IL-1beta, IL-6, TNF-alpha and IL-23 induced by IL-36 in BMDC and CD4 T cells. Skin and dendritic cells are targets of the IL-36 interleukins leading to a Th1 response. These cytokines may represent potential targets for immune-mediated inflammatory conditions or, alternatively, could be used as adjuvants in vaccination.

Supplier: Adipogen

Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-alpha, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-alpha (MultimericTNF-alpha (Prod. No. AG-40B-0019). Since the MultimericTNF-alpha mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-alpha protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-alpha or MultimericTNF-alpha can be used.

Supplier: STEMCELL Technologies

R-Spondin-3 is a member of thrombospondin type 1 repeat (TSR-1) superfamily that is involved in the canonical Wnt/β-catenin signaling pathway (de Lau et al.). R-spondin proteins are characterized by two furin-like repeats at the amino terminus and thrombospondin domain located near the carboxyl terminus (de Lau et al.). R-spondin-3 expression is associated with ovarian cancer (Gu et al.), prostate cancer (Mesci et al.), and differentiation of intestinal epithelial cells in diabetes mellitus (Shan et al.). In a transgenic mice model, the expression of R-Spondin-3 induces the expansion of Lgr5+ stem cells, Paneth cells, and Lgr4+ cells, promoting the intestinal stem cell compartment (Hilkens et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain.