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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

Rainbow Calibration Particles, 6 peaks (3.0-3.4 μM), BioLegend

Rainbow Calibration Particles, 6 peaks (3.0-3.4 μM), BioLegend

Supplier: Biolegend

Rainbow Calibration Particles 6 peaks (3.0-3.4 uM) ; Apps: FC

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Cyto-Last™ Buffer, BioLegend

Cyto-Last™ Buffer, BioLegend

Supplier: Biolegend

Cyto-Last* Buffer; Apps: ICFC

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Calbiochem® ImmunoPen™, MilliporeSigma

Supplier: MilliporeSigma

ImmunoPen™ is designed to minimize waste of antibody in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. When applied in a circle around the specimen, forms a hydrophobic barrier, retaining antibody.

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Rainbow Fluorescent Particles, 1 peak (3.0-3.4 μM) - Mid Range Intensity, BioLegend

Rainbow Fluorescent Particles, 1 peak (3.0-3.4 μM) - Mid Range Intensity, BioLegend

Supplier: Biolegend

Rainbow Fluorescent Particles 1 peak (3.0-3.4 uM) - Mid Range Intensity ; Apps: FC

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Rainbow Fluorescent Particles, 1 peak (3.0-3.4 μM) - Bright Intensity, BioLegend

Rainbow Fluorescent Particles, 1 peak (3.0-3.4 μM) - Bright Intensity, BioLegend

Supplier: Biolegend

Rainbow Fluorescent Particles 1 peak (3.0-3.4 uM) - Bright Intensity; Apps: FC

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Rainbow Calibration Particles, 8 peaks (3.0-3.4 μM), BioLegend

Rainbow Calibration Particles, 8 peaks (3.0-3.4 μM), BioLegend

Supplier: Biolegend

Rainbow Calibration Particles 8 peaks (3.0-3.4 uM); Apps: FC

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ReadiLink™ Rapid Cy5 Antibody Labelling Kit

ReadiLink™ Rapid Cy5 Antibody Labelling Kit

Supplier: AAT Bioquest

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with Cy5.

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Helixyte™ iFluor® 488 Nucleic Acid Labeling Dye *Optimized for Labeling 2×100 µg DNA/RNA*

Helixyte™ iFluor® 488 Nucleic Acid Labeling Dye *Optimized for Labeling 2×100 µg DNA/RNA*

Supplier: AAT Bioquest

Helixyte™ iFluor® 488 Nucleic acid labeling dye is a key member of our enabling Helixyte™ nucleic acid labeling and conjugation technology.

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Accessories for Calmodulin Resin for CBP Purification, G-Biosciences

Supplier: G-Biosciences

(50mM Tris-HCl, pH 7.5, 200mM NaCl, 2mM EGTA), 100ml

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ReadiLink™ Rapid iFluor® 488 Antibody Labelling Kit

ReadiLink™ Rapid iFluor® 488 Antibody Labelling Kit

Supplier: AAT Bioquest

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with our superior iFluor® dyes.

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Wheat Germ Agglutinin, XFD750 Labeled *XFD750 Same Structure to Alexa Fluor® 750*

Wheat Germ Agglutinin, XFD750 Labeled *XFD750 Same Structure to Alexa Fluor® 750*

Supplier: AAT Bioquest

XFD750, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 750, a trademark of ThermoFisher.

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ReadiLink™ Rapid XFD594 Antibody Labelling Kit

ReadiLink™ Rapid XFD594 Antibody Labelling Kit

Supplier: AAT Bioquest

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with XFD dyes (equivalent to Alexa Fluor®).

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HOOK™ Dye Labeling Kit (FITC) for Labeling Antibodies & Proteins with Fluorescent Dyes, G-Biosciences

HOOK™ Dye Labeling Kit (FITC) for Labeling Antibodies & Proteins with Fluorescent Dyes, G-Biosciences

Supplier: G-Biosciences

Fluorescein Isothiocynate (FITC) is the simplest and most commonly used fluorescent dye for labeling of proteins, peptides, and antibodies

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Buccutite™ Peroxidase (HRP) Antibody Conjugation Kits (2 Labelings), AAT Bioquest

Buccutite™ Peroxidase (HRP) Antibody Conjugation Kits (2 Labelings), AAT Bioquest

Supplier: AAT Bioquest

Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins.

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Buccutite™ ALP (Alkaline Phosphatase) Antibody Conjugation Kits (2 Labelings), AAT Bioquest

Buccutite™ ALP (Alkaline Phosphatase) Antibody Conjugation Kits (2 Labelings), AAT Bioquest

Supplier: AAT Bioquest

Protein-protein conjugations are commonly performed with a bifunctional linker (such as SMCC) having different reactivity at each end for linking two different proteins.

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ReadiLink™ Rapid iFluor® 350 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* (2 Labelings), AAT Bioquest

ReadiLink™ Rapid iFluor® 350 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* (2 Labelings), AAT Bioquest

Supplier: AAT Bioquest

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies.

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SNAP-SURFACE® 488 , New England Biolabs

SNAP-SURFACE® 488 , New England Biolabs

Supplier: New England Biolabs (NEB)

SNAP-Surface® 488 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells

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Buccutite™ Rapid APC-Cy5.5 Tandem Antibody Labelling Kit

Buccutite™ Rapid APC-Cy5.5 Tandem Antibody Labelling Kit

Supplier: AAT Bioquest

Production scale optimized for labelling 1 mg antibody per reaction. Buccutite™ Rapid APC-Cy5.5 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

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Buccutite™ Rapid PE-Cy7 Tandem Antibody Labelling Kit

Buccutite™ Rapid PE-Cy7 Tandem Antibody Labelling Kit

Supplier: AAT Bioquest

Production scale optimized for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Cy7 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

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ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* (2 Labelings), AAT Bioquest

ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* (2 Labelings), AAT Bioquest

Supplier: AAT Bioquest

Cy7 is one of the most popular fluorescent labeling dyes for preparing orange-red fluorescent bioconjugates.

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Affinity Purified Avidin, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' avidin is a glycoprotein with approximately 10% of its total mass comes from carbohydrates

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ReadiLink™ iFluor® 555 FISH Fluorescence Imaging Kit

ReadiLink™ iFluor® 555 FISH Fluorescence Imaging Kit

Supplier: AAT Bioquest

The kit provides Taq DNA polymerase enzyme which incorporates iFluor® 555-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).

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SNAP-CELL® Oregon Greeen , New England Biolabs

SNAP-CELL® Oregon Greeen , New England Biolabs

Supplier: New England Biolabs (NEB)

SNAP-Cell Oregon Green is a photostable green fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells.

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Mix-n-Stain™ Nanobody Labeling Kits, Biotium

Mix-n-Stain™ Nanobody Labeling Kits, Biotium

Supplier: Biotium

The simplicity and convenience of Mix-n-Stain™ labeling of nanobodies with fluorescent CF® dyes or biotin.

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Carboxyl Coupling Resin, G-Biosciences

Carboxyl Coupling Resin, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' Carboxyl Coupling Resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm

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CF®DI Hydrazide, Biotium

Supplier: Biotium

CF®DI dye hydrazides react with molecules containing aldehyde or ketone groups to form a hydrazone bond. The dyes are size- and charge-matched and do not significantly alter the molecular weight or pI of the labeled proteins. They are used for detection of protein carbonylation by fluorescence 2D-DIGE.

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HaloTag Biotin Ligand, 15 µl, Promega

HaloTag Biotin Ligand, 15 µl, Promega

Supplier: Promega Corporation

The HaloTag Technology enables covalent protein labeling and immobilization in vivo and in vitro.

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Reactive Oxygen Species Indicators, Biotium

Supplier: Biotium

Commonly used fluorescent dyes for detection of oxidative activities in cells.

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HOOK™ Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine) for Labeling Antibodies & Proteins with Fluorescent Dyes, G-Biosciences

HOOK™ Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine) for Labeling Antibodies & Proteins with Fluorescent Dyes, G-Biosciences

Supplier: G-Biosciences

(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides.

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HaloTag TMRDirect Ligand, 30 µl, Promega

HaloTag TMRDirect Ligand, 30 µl, Promega

Supplier: Promega Corporation

The HaloTag Technology enables covalent protein labeling and immobilization in vivo and in vitro.

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