You searched for: Protein Linking and Labeling Systems

Supplier: Invitrogen

Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (biotin-N4-CTP) onto the 3' end of DNA strands where the label will be less likely to interfere with hybridization or sequence-specific binding of proteins.

Supplier: Invitrogen

DyLight™ Dyes have absorption spectra ranging from 350 nm to 770 nm and match the output wavelengths of common fluorescence instrumentation

Supplier: Invitrogen

TMT10plex™ Isobaric Mass Tag Labeling Reagents Sets enable multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS).

Supplier: Abnova

Fluorescence Dye 488-I conjugates are significantly brighter than fluorescein conjugates and much more photostable.

Supplier: Invitrogen

Introduce free sulfhydryl groups into a protein of interest to allow the modified protein to react with a variety of sulfhydryl-selective products.

Supplier: Invitrogen

The iodoTMTzero label reagent can be used as an isotopic 'light' duplex tag for MS-level protein quantitation.

Supplier: Surmodics

StabilZyme® AP and StabilZyme® Protein-Free AP stabilizers provide assay developers a complete set of tools to stabilize different AP conjugates for long term, in-solution stability.

Supplier: Invitrogen

The Thermo Scientific Pierce RNA 3'-End Desthiobiotinylation Kit contains reagents for easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.

Supplier: Invitrogen

Thermo Scientific™ Pierce Fluorescence Biotin Quantitation Kit requires only ten microliters of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.

Supplier: Invitrogen

The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) label reagents enable multiplexed characterization and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidized proteins) by mass spectrometry (MS).

Supplier: Invitrogen

Thermo Scientific iodoTMTsixplex Label Reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry.

Supplier: Anaspec Inc

HiLyte™ Fluor 488, SE is an excellent amine-reactive fluorescent labeling dye for generating protein conjugates. The spectrum of HiLyte™ Fluor488 is similar to fluorescein (FITC), resulting in an optimal match to filters designed for fluorescein.

Supplier: Invitrogen

Photoactivatable Biotin, Biotin-LC-ASA, Psoralen-PEG3-Biotin and TFPA-PEG3-Biotin each contain a photoactivatable group. When exposed to UV light, they become activated and insert non specifically into nearby molecules. These reagents may be used to label proteins and peptides, but they are also useful in labeling DNA, RNA and other molecules that do not contain any readily reactive functional groups.

Supplier: Invitrogen

Activated peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins.

Supplier: Invitrogen

Thermo Scientific DyLight Fluor Phosphine Reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.

Supplier: Invitrogen

Thermo Scientific Pierce Maleimide Activated Horseradish Peroxidase is for preparation of HRP conjugates with proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.

Supplier: Invitrogen

The Thermo Scientific Pierce RNA 3' End Biotinylation Kit is optimized for labeling the 3-prime terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions.

Supplier: BIOPOINT USA INC MS

EasyStain™ is an immunofluorescence reagent designed for use in testing water samples for the presence of Cryptosporidium oocysts and Giardia cysts.

Supplier: Surmodics

StabilZyme® AP and StabilZyme® Protein-Free AP stabilizers provide assay developers a complete set of tools to stabilize different AP conjugates for long term, in-solution stability.

Supplier: Invitrogen

The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 - 145 nm in excitation/emission wavelengths.

Supplier: Invitrogen

The Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit provides researchers with a streamlined, robust method to enrich protein-RNA interactions using end-labeled RNA as the bait.

Supplier: Invitrogen

Sulfo-SBED is a trifunctional crosslinking reagent containing a biotin, a sulfonated N-hydroxysuccinimide (Sulfo-NHS) active ester and a photoactivatable aryl azide

Supplier: Anaspec Inc

HiLyte™ Fluor 555 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates that are only slightly red-shifted compared to those of Cy™ 3 dyes, resulting in an optimal match to filters designed for CyTM dyes.

Supplier: Surmodics

StabilZyme® HRP, StabilZyme® Protein-Free and StabilZyme® NOBLE stabilizers provide the assay developer a complete set of tools to stabilize different HRP conjugates for long term, in-solution stability.

Supplier: Invitrogen

Thermo Scientific iodoTMTsixplex Isobaric Mass Tag Labeling Kit contains iodoTMT™ reagents and associated materials for the irreversible labeling of sulfhydryl groups.

Supplier: Invitrogen

The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.

Supplier: Anaspec Inc

Biotin, SE is amino-reactive biotinylating reagent for peptides and proteins; it has a better avidin-binding affinity than biotin. Biotin, SE (d-Biotin-N-hydroxysuccinimide ester) contains a five-carbon spacer arm that reduces steric hindrance associated with binding four biotinylated molecules per one avidin and results in enhanced detection sensitivity.

Supplier: Anaspec Inc

Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. 5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivity to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels.

Supplier: Invitrogen

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: Invitrogen

Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatized biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4.5 - 5 and requires just minutes to complete.