Protein Extraction
Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.
Pierce™ Mitochondria Isolation Kit for Cultured Cells, Thermo Scientific
Supplier: Invitrogen
The Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples.
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Pierce Imject™ Maleimide-Activated Ovalbumin, Thermo Scientific
Supplier: Invitrogen
Imject™ Maleimide-activated ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.
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Pierce™ Rapid Mouse Antibody Isotyping Kit, Thermo Scientific
Supplier: Invitrogen
The Pierce™ Rapid Isotyping Kit is a 5-minute lateral-flow assay with ELISA sensitivity for class and subclass determination of monoclonal antibodies.
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Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.
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Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific
Supplier: Invitrogen
Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling
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Pierce™ Immobilized Papain (Agarose Resin), Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Immobilized Papain consists of a cysteine-endopeptidase (papain) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
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ReadyPrep Reagents for Extraction and Solubilization, Bio-Rad
Supplier: Bio-Rad
A number of ReadyPrep kits are available for total protein or targeted protein extraction (fractionation)
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Imject™ Pierce™ Maleimide-Activated Carrier Proteins, Thermo Scientific
Supplier: Invitrogen
Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten
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Imject™ Pierce™ Maleimide-Activated BSA Spin Kit, Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific Imject Maleimide-Activated BSA Spin Kit contains stabilized, lyophilized Maleimide-Activated BSA (in PBS with stabilizer) and accessory components to easily prepare ready-to-use immunogens for immunization.
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Pierce™ Mem-PER™ Plus Membrane Protein Extraction Kit, Thermo Scientific
Supplier: Invitrogen
The Mem-PER™ Plus Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. It effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.
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Pierce™ Mitochondria Isolation Kit for Tissue, Thermo Scientific
Supplier: Invitrogen
The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples.
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Pierce™ In-Solution Tryptic Digestion and Guanidination Kit, Thermo Scientific
Supplier: Invitrogen
Pierce™ In-Solution Tryptic Digestion and Guanidination Kit contains proteomics grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.
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B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific
Supplier: Invitrogen
B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.
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Pierce™ Surfact-Amps™ Detergent Solutions, Thermo Scientific
Supplier: Invitrogen
Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques.
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Pierce™ Detoxi-Gel™ Endotoxin Removing Gel and Columns, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Detoxi-Gel Endotoxin Removing Gel uses Polymixin B ligand immobilized on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.
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Pierce™ Endoproteinase Lys-C Protease, MS Grade, Thermo Scientific
Supplier: Invitrogen
Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
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