Protein Extraction

Supplier: G-Biosciences

A water soluble, odorless, non-toxic and stable protein reductant, G-Biosciences' Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol)

Supplier: New England Biolabs (NEB)

Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg

Supplier: G-Biosciences

G-Biosciences' FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.

Supplier: Immuno4 LLC

Developed specifically for ELISA and In-Vitro Diagnostic applications requiring ultra sensitivity with exceptional signal to noise ratios.

Supplier: Promega Corporation

FastBreak Cell Lysis Reagent allows efficient, gentle lysis of E. coli cultures without the need for centrifugation or mechanical disruption.

Supplier: Promega Corporation

The TNT Coupled Wheat Germ Extract Systems are one-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

Supplier: Promega Corporation

TNT Coupled Reticulocyte Lysate Systems are single-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

Supplier: G-Biosciences

G-Biosciences' Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose

Supplier: G-Biosciences

A complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples

Supplier: G-Biosciences

G-Biosciences' Total Protein Extraction (TPE™) Kit is a universal lysis system for the solubilization of total proteins from animal, plant, yeast, bacteria, and other biological samples

Supplier: Avanti Polar Lipids Inc

Polar lipid extract is derived from the total lipid extract by precipitation with acetone followed by extraction of the acetone insoluble material with diethyl ether.

Supplier: Invitrogen

Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.Easy-to-use—resin provided in single-use spin column formatImproved protein identifications—protein identifications increased by ≥50% when compared to unfractionated samplesReproducible—elution profiles and fractional resolution vary by less than 20%Optimized—robust procedure for maximal protein identification and peptide recovery while minimizing fractional overlapCompatible—reagents validated with a variety of complex samples, including TMT™-labeled peptidesIn order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity and provides excellent orthogonality to low pH reversed-phase LC-MS gradients.The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a highpH fractionation protocol. In contrast to strong cation exchange (SCX) fractionation, the high-pH reversed-phase fractions do not require an additional desalting step before LC/MS analysis.The High pH Reversed-phase Peptide Fractionation Kit includes a high pH buffer (0.1% triethylamine) and twelve spin columns containing pH-resistant reversed-phase resin. Each reversed-phase fractionation spin column enables fractionation of 10–100 µg of peptide sample using a microcentrifuge.Native, phosphorylated, Tandem Mass TagTM (TMTTM)-labeled, and other complex peptide mixture samples can be fractionated using this kit. Combining the search results generated by the individual fractions helps improve protein sequence coverage and increases the number of identified proteins relative to unfractionated samples.Applications:Reducing sample complexity to identify targets of interestConducting systems biology studiesReducing sample complexity to improve quantitation studies

Supplier: Invitrogen

The Mem-PER™ Eukaryotic Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of hydrophobic membrane-associated proteins in a simple reagent-based procedure

Supplier: Bio-Rad

The ReadyPrep protein extraction kit (cytoplasmic/nuclear) prepares fractions enriched in cytoplasmic and nuclear proteins from eukaryotic samples, such as cultured cells and tissues.

Supplier: Invitrogen

Imject™ Mariculture KLH (mcKLH) is a purified keyhole limpet haemocyanin carrier protein that enables simple preparation of highly effective immunogens with peptide antigens.

Supplier: Invitrogen

Chymotrypsin Protease, MS Grade, is a purified native endoproteinase validated for maximum activity and selectivity in proteomic and used in in-solution digestions and in-gel digestion of proteins from from 1-D or 2-D gels. Store at -20˚C in a nonfrost-free freezer.