Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

EndoTrap® HD 1/1, Biovendor

Supplier: BioVendor

EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.

The EndoTrap® HD resin is especially designed to meet the needs of pharma and biotech industry for the efficient removal of endotoxins in biomanufacturing processes as well as any application that requires highest endotoxin removal rates.

EndoTrap® HD fulfils all requirements for industrial applications, and it can easily be integrated in/at early or late-stage purification processes. Moreover, the High-Definition Matrix is also available for small-scale purification as pre-filled columns.

Endotoxin removal with EndoTrap® HD works effectively in the pH range of 4-9 and NaCl concentrations of 50-600 mM. Buffers such as HEPES, TRIS, MOPS, MES, and PIPES are recommended. Citrate buffers and chelators of divalent cations (like EDTA) have to be avoided

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Hank's Balanced Salt Solution (HBSS), Modified (Without Ca⁺⁺ and Mg⁺⁺)

Supplier: Stemcell Technologies

Hank's Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source.

10X Citrate Buffers, Novus Biologicals

Supplier: Novus Biologicals

10X Citrate Buffer.

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Saline Solution, Sterile, 0.9%, Moltox

Supplier: MOLTOX Molecular Toxicology

Cell culture buffer, 0.9% saline solution

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Bis-tris propane/ Hydrochloric acid, buffer, 1M, Rigaku

Supplier: Rigaku Reagents

Bis-tris propane/ Hydrochloric acid, buffer, 1M, Rigaku

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AdvanWash™ washing solution, Advansta

Supplier: ADVANSTA INC MS

Optimized washing solutions, for Western blotting with WesternBright systems.

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Phosphate Buffered Saline/Casein Block & Diluent-5X, BioFX®, Surmodics IVD, Inc.

Supplier: Surmodics

BioFX® Phosphate Buffered Saline/Casein Block and Diluent-5X Concentrate minimizes non-specific interactions of the secondary reagents with each other and the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. The solution is ready for dilution to specific application requirements.