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Buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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10X Tris-Glycine

Supplier: Rockland Immunochemical

Tris-Glycine Running Gel buffer for polyacrylamide gel electrophoresis - without SDS (sodium dodecyl sulfate)

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10X Tris-Acetate-EDTA (TAE)

Supplier: Rockland Immunochemical

10X Tris-Acetate-EDTA (TAE) Buffer

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SSPE Buffer, 20X, Rockland Immunochemicals

Supplier: Rockland Immunochemical

Saline–Sodium Phosphate–EDTA (SSPE) is used in bacterial screening and hybridization procedures.

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20X SSC pH 7.0

Supplier: Rockland Immunochemical

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridization process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridization, or DNA microarrays).

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Sodium Dodecyl Sulfate SDS, 20%

Supplier: Rockland Immunochemical

SDS, sodium dodecyl sulfate is an anionic surfactant.

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1X TBS pH 7.8

Supplier: Rockland Immunochemical

1X Tris buffered saline (TBS) pH 7.8

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BlockOut® Universal Blocking Buffer for Western Blotting, Rockland Immunochemicals

Supplier: Rockland Immunochemical

BlockOut® is an universal blocking buffer solution for Western Blotting. It is specifically designed for colorimetric, chemiluminescent, and fluorescent western blotting.

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RIPA Lysis Buffer, Rockland Immunochemicals

Supplier: Rockland Immunochemical

RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.

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Cell Lysis Buffer, Rockland Immunochemicals

Supplier: Rockland Immunochemical

Cell Lysis Buffer is used to lyse cells under non-denaturing conditions.

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1X PBS Buffer with 1% Casein

Supplier: Rockland Immunochemical

Phosphate buffered saline with casein is suitable for multiple applications including biological diluent buffer for antibodies or other biologics.

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1X TBS Buffer with 1% Casein

Supplier: Rockland Immunochemical

Tris buffered saline with casein is suitable for multiple applications including biological diluent buffer for antibodies or other biologics.

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FACS and IF Staining Buffer with FBS

Supplier: Rockland Immunochemical

Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer with FBS

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FACS and IF Staining Buffer with Goat Serum

Supplier: Rockland Immunochemical

Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer with Goat Serum

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FACS and IF Staining Buffer with BSA

Supplier: Rockland Immunochemical

Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer with BSA

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Normal Saline

Supplier: Rockland Immunochemical

This solution is commonly referred to as physiological saline or isotonic saline.

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Borate Buffered Saline (BBS), Rockland Immunochemicals

Supplier: Rockland Immunochemical

Borate buffered saline (BBS) is commonly used in coating procedures for ELISA.

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