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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

TBE running buffer (10X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

2L Liquid

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TRIS-Tricine-SDS running buffer (10X), cathode buffer, pH 8.3, Thermo Scientific

Supplier: Thermo Scientific Chemicals

For TRIS-Tricine SDS gel electrophoresis

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HEPES Buffered Saline, Promocell

Supplier: Promocell, Inc.

These solutions are prepared from raw materials of the highest quality using tissue culture grade water.

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BSA Blocking Buffer, 5% in TBS, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Casein Blocking Buffer, 3% in TBS, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Imidazole-buffered saline (5X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Nitrocellulose stripping buffer (10X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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BSA Blocking Buffer, 3% in PBS, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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IEF Anode buffer (50X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

For isoelectric focusing (IEF)

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MNE buffer, pH 6.5, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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RIPA buffer with EDTA + EGTA, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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RBC lysis buffer for human, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Monarch® RNA Cleanup Wash Buffer, New England Biolabs

Monarch® RNA Cleanup Wash Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

This is a component of the Monarch® RNA cleanup kits, is used to wash RNA after binding to ensure the removal of contaminants such as enzyme, NTPs, and short RNA fragments. It is supplied as a 5X concentrate and requires dilution with ethanol (≥95%) before use.

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Phosphate Buffer, BD Diagnostics

Supplier: BD

Phosphate Buffer, BD Diagnostics

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Monarch® gDNA Elution Buffer II, New England Biolabs

Monarch® gDNA Elution Buffer II, New England Biolabs

Supplier: New England Biolabs (NEB)

The Monarch® gDNA elution buffer II is supplied as a component of the Monarch HMW DNA extraction kits.

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RIPA buffer with Triton® X-100 (1X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Phosphate Buffer Solution, for Chlorine Determination (DPD Methods), Ricca Chemical Company
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Extraction Reagents 1 (5X), (10 ml), Enzo Life Sciences

Supplier: Enzo Life Sciences

Concentrated lysis buffer for preparation of cell and tissue extracts.

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Extraction Reagents 2 (5X), (10 ml), Enzo Life Sciences

Supplier: Enzo Life Sciences

Concentrated lysis buffer for preparation of cell and tissue extracts.

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MOPS-SDS running buffer (20X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

1L Liquid

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ACES, 0.5 M Buffer Solutions, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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CHES, 0.5 M Buffer Solutions, Thermo Scientific

Supplier: Thermo Scientific Chemicals

2-(Cyclohexylamino)ethanesulfonic acid, CHES.

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TBE Buffer, 10X, Molecular Biology Grade, Promega

TBE Buffer, 10X, Molecular Biology Grade, Promega

Supplier: Promega Corporation

TBE Buffer, 10X (pH 8.3), is used for polyacrylamide and agarose gel electrophoresis.

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Alkaline Phosphatase Buffer-1 (5X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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NEBuffer r2.1, New England Biolabs

NEBuffer r2.1, New England Biolabs

Supplier: New England Biolabs (NEB)

New England Biolabs provides a color-coded 10 X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. These buffers contain Recombinant Albumin (NEB #B9200).

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Casein Blocking Buffer, 3% in PBS, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Section Block, Electron Microscopy Sciences

Supplier: Electron Microscopy Sciences

Blocking solutions based on chemically modified and fragmented ultra-pure casein. Effectively reduces unwanted binding of primary antibody and conjugates use to charged surface of the slide and tissue section.

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Sodium Citrate Buffer, 0.5 M, pH 4.5, 250 ml, RNase/DNase Tested, Sterile

Supplier: Teknova

Sodium Citrate Buffer, 0.5 M, pH 4.5, 250 ml, RNase/DNase Tested, Sterile

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Phosphate Buffer, pH 6.5, Electron Microscopy Sciences

Supplier: Electron Microscopy Sciences

Wright-Giemsa Stain, Lillie (1972), For differentiation of blood corpuscles, Stain results - Erthrocytes: Pink-Tan, Eosinophiles -Granules: Red, Lymphocytes -Granules: Red-Purple, -Cytoplasm: Blue

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