Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
TBE running buffer (10X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
2L Liquid
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TRIS-Tricine-SDS running buffer (10X), cathode buffer, pH 8.3, Thermo Scientific
Supplier: Thermo Scientific Chemicals
For TRIS-Tricine SDS gel electrophoresis
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HEPES Buffered Saline, Promocell
Supplier: Promocell, Inc.
These solutions are prepared from raw materials of the highest quality using tissue culture grade water.
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BSA Blocking Buffer, 5% in TBS, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Casein Blocking Buffer, 3% in TBS, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Imidazole-buffered saline (5X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Nitrocellulose stripping buffer (10X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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BSA Blocking Buffer, 3% in PBS, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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IEF Anode buffer (50X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
For isoelectric focusing (IEF)
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RIPA buffer with EDTA + EGTA, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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RBC lysis buffer for human, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Monarch® RNA Cleanup Wash Buffer, New England Biolabs
Supplier: New England Biolabs (NEB)
This is a component of the Monarch® RNA cleanup kits, is used to wash RNA after binding to ensure the removal of contaminants such as enzyme, NTPs, and short RNA fragments. It is supplied as a 5X concentrate and requires dilution with ethanol (≥95%) before use.
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Monarch® gDNA Elution Buffer II, New England Biolabs
Supplier: New England Biolabs (NEB)
The Monarch® gDNA elution buffer II is supplied as a component of the Monarch HMW DNA extraction kits.
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RIPA buffer with Triton® X-100 (1X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Phosphate Buffer Solution, for Chlorine Determination (DPD Methods), Ricca Chemical Company
Supplier: Ricca Chemical
APHA for chlorine (DPD method).
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Sodium Bicarbonate, 1 M Buffer Solution, pH 10.0, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Extraction Reagents 1 (5X), (10 ml), Enzo Life Sciences
Supplier: Enzo Life Sciences
Concentrated lysis buffer for preparation of cell and tissue extracts.
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Extraction Reagents 2 (5X), (10 ml), Enzo Life Sciences
Supplier: Enzo Life Sciences
Concentrated lysis buffer for preparation of cell and tissue extracts.
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MOPS-SDS running buffer (20X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
1L Liquid
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ACES, 0.5 M Buffer Solutions, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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CHES, 0.5 M Buffer Solutions, Thermo Scientific
Supplier: Thermo Scientific Chemicals
2-(Cyclohexylamino)ethanesulfonic acid, CHES.
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TBE Buffer, 10X, Molecular Biology Grade, Promega
Supplier: Promega Corporation
TBE Buffer, 10X (pH 8.3), is used for polyacrylamide and agarose gel electrophoresis.
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Alkaline Phosphatase Buffer-1 (5X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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NEBuffer r2.1, New England Biolabs
Supplier: New England Biolabs (NEB)
New England Biolabs provides a color-coded 10 X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. These buffers contain Recombinant Albumin (NEB #B9200).
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Casein Blocking Buffer, 3% in PBS, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Section Block, Electron Microscopy Sciences
Supplier: Electron Microscopy Sciences
Blocking solutions based on chemically modified and fragmented ultra-pure casein. Effectively reduces unwanted binding of primary antibody and conjugates use to charged surface of the slide and tissue section.
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Sodium Citrate Buffer, 0.5 M, pH 4.5, 250 ml, RNase/DNase Tested, Sterile
Supplier: Teknova
Sodium Citrate Buffer, 0.5 M, pH 4.5, 250 ml, RNase/DNase Tested, Sterile
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Phosphate Buffer, pH 6.5, Electron Microscopy Sciences
Supplier: Electron Microscopy Sciences
Wright-Giemsa Stain, Lillie (1972), For differentiation of blood corpuscles, Stain results - Erthrocytes: Pink-Tan, Eosinophiles -Granules: Red, Lymphocytes -Granules: Red-Purple, -Cytoplasm: Blue