You searched for: Antibodies

Supplier: Rockland Immunochemical

This protein-A purified Antibody has been tested for use in Western blotting (33.9 kDa band expected) and is specific for Borrelia burgdorferi Flagellin protein.

Supplier: Rockland Immunochemical

COX, also known as cytochrome c oxidase, has 12 subunits that make up the transmembrane mitochondrial protein. Subunit IV has two isoforms; COXIV-1 and COXIV-2. COXIV-1 is expressed ubiquitously while COXIV-2 is highly expressed in adult lung and low levels in brain and heart. Phosphorylation of amino acid residue ser58 of the COXIV-1 protein is a PKA-dependent regulation of COX and plays an important role in metabolism and CREB cycle activation. COX IV pS58 Antibody is ideal for researchers interested in cell cycle research.

Supplier: Rockland Immunochemical

APP antibody detects the human amyloid A4 protein. Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the brains of all suffers of Alzheimer's disease. Anti-APP is ideal for investigators that are involved in Neuroscience research.

Supplier: Rockland Immunochemical

Anti-Retinoid X Receptor gamma antibody is suitable for use in Western Blotting to detect a single band at approximately 48 kDa corresponding to the RXR-gamma isotype in the appropriate cell lysate or extract.

Supplier: Rockland Immunochemical

Anti-Raf 1 pS642 antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 74 kDa in size corresponding to Raf-1 Protein phosphorylated at Ser642 in the appropriate cell lysate.

Supplier: Rockland Immunochemical

F(ab')2 Bovine IgG F(ab')2, Polyclonal antibody, Host: Rabbit, Label: Peroxidase (Horseradish), Store vial at 4degree C prior to restoration, Concentration: 10.0 mg/mL by UV absorbance at 280 nm, Application: Elisa, WB, Ihc, 20mg

Supplier: Rockland Immunochemical

F(ab')2 Horse IgG (HandL), Polyclonal antibody, Host: Rabbit, Label: Hrp, Store vial at 4degree C prior to restoration, Application: Elisa, Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays, 20mg

Supplier: Rockland Immunochemical

F(ab')2 Bovine IgG (H&L), Polyclonal antibody, Host: Rabbit, Target Species: Bovine, Format: IgG F(ab')2, Immunogen: Bovine IgG whole molecule, Applications: immunomicroscopy and flow cytometry or FACS analysis, antibody based fluorescent assays, Size: 20mg

Supplier: Rockland Immunochemical

This affinity purified antibody has been tested for use in ELISA and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 72 kDa, 71.5 kDa or 65.9 kDa in size corresponding to isoforms of APC6 by western blotting.

Supplier: Rockland Immunochemical

Anti-NOXO1 antibody has been tested for use in ELISA, immunochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to NOXO1 Protein by western blot.

Supplier: Rockland Immunochemical

GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. GDNF is a small protein that potently promotes the survival of many types of neurons. GDNF is a founding member of the GDNF family of ligands (GFL).The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization (FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. This antibody is suitable for researchers interested in neuroscience research.

Supplier: Rockland Immunochemical

This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a single band approximately 55 kDa in size corresponding to PPARg2 by western blot.

Supplier: Rockland Immunochemical

Anti-DARPP-32 pS137 Antibody is suitable for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 32 kDa in size corresponding to DARPP 32 Protein phosphorylated at Ser13.

Supplier: Rockland Immunochemical

Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.

Supplier: Rockland Immunochemical

Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.

Supplier: Rockland Immunochemical

Interleukin 1 receptor type II binds to IL-1 alpha, IL-1 beta and IL1R1/IL1RA. Anti-IL-1 Receptor Type II antibody is ideal for investigators involved in Immunology and Stem Cell research.

Supplier: Rockland Immunochemical

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFN-gamma induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1. Ectopicly expressed DAPK2 induced apoptosis in various types of cells. DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues.

Supplier: Rockland Immunochemical

Anti-Rabpihilin 3A pS234 (Rabbit) antibody is suitable for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 82 kDa in size corresponding to rabphilin 3A Protein phosphorylated at Ser234.

Supplier: Rockland Immunochemical

6X His Tag CY3 conjugated Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.

Supplier: Rockland Immunochemical

F(ab')2 Chicken IgG (H&L), Polyclonal antibody, Host: Rabbit, Target Species: Chicken, Format: IgG F(ab')2, Label: Fluorescein (FITC), Immunogen: Chicken IgG whole molecule, Applications: immunomicroscopy and flow cytometry or FACS analysis, Synonym: FITC, Size: 20mg

Supplier: Rockland Immunochemical

F(ab')2 Anti-CHICKEN IgG F(ab')2, Polyclonal antibody, Host: Rabbit, Target Species: Chicken, Format: IgG F(ab')2, Label: Peroxidase (Horseradish), Immunogen: Chicken IgG F(ab')2 fragment, Synonyms: HRP, Applications: ELISA, WB, IHC, Size: 20mg

Supplier: Rockland Immunochemical

Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS'.

Supplier: Rockland Immunochemical

Similar to its C. elegans homolog UNC-76, mammalian FEZ1, an abundant membrane protein and protein kinase C (PKC)-Z substrate, is involved in axonal guidance. Following phosphorylation by PKC-Z, FEZ1 translocates from the plasma membrane to the cytoplasm. FEZ1 is then able to interact with a number of proteins involved in axonal outgrowth and cellular transport such as DISC1, a candidate gene for schizophrenia, as well as kinesin-1, a microtubule-based motor protein. FEZ1 also interacts with proteins involved in transcriptional regulation and chromatin remodeling, suggesting that FEZ1 may have nuclear regulatory functions in addition to those for neuronal development. At least two distinct isoforms of FEZ1 are known to exist.

Supplier: Rockland Immunochemical

The highly conserved, nuclear-localized Akirin1 and Akirin2 proteins critically regulate the transcription of NF-kB-dependent genes and are required for defense against Gram-negative bacteria in the immune deficiency and NF-kB pathways. Akirin1 is dispensable in the mouse, and neither knockout mice nor cells derived from them have obvious distinctive phenotypes. In contrast, Akirin2 is required for development in the mouse and knockout of both Akirin homologs in mice show that Akirin2 is required downstream of toll-like receptor (TLR), TNF-a and IL-1b signaling, and for the production of IL-6. Akirin2 is functionally closer to the single gene in Drosophila, as the homozygous null D. melanogaster Akirin mutants show a similar, mid-to-early embryonic death.

Supplier: Rockland Immunochemical

5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells). Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics: 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies. Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. Anti-5hmC Antibody is ideal for research in Epigenetics and Gene Expression.

Supplier: Rockland Immunochemical

The highly conserved, nuclear-localized Akirin1 and Akirin2 proteins critically regulate the transcription of NF-kB-dependent genes and are required for defense against Gram-negative bacteria in the immune deficiency and NF-kB pathways. Akirin1 is dispensable in the mouse, and neither knockout mice nor cells derived from them have obvious distinctive phenotypes. In contrast, Akirin2 is required for development in the mouse and knockout of both Akirin homologs in mice show that Akirin2 is required downstream of toll-like receptor (TLR), TNF-a and IL-1b signaling, and for the production of IL-6. Akirin2 is functionally closer to the single gene in Drosophila, as the homozygous null D. melanogaster Akirin mutants show a similar, mid-to-early embryonic death.

Supplier: Immunoreagents

Igg (H&L), Affinity Pure, Polyclonal antibody, Host: LlamaSpecies: Mouse, Immunogen: Purified Mouse, IgG, whole molecule, Preservative: 0.05% (w/v) Sodium Azide, Applications: immunomicroscopy, flow cytometry

Supplier: Rockland Immunochemical

This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 170 kDa in size corresponding to EGFR Protein by western blotting.

Supplier: Rockland Immunochemical

Aipl1 was initially identified as a protein implicated in Leber congenital amaurosis (LCA), an autosomal recessive disorder thought to be caused by the abnormal development of photoreceptors. Aipl1 is a tetratricopeptide repeat protein that is highly homologous to ARA9, a protein involved in the HSP90-mediated nuclear translocation and transactivation of the aryl hydrocarbon receptor. Aipl1 has also been found to function as part of a chaperone heterocomplex, interacting with Hsp90 and Hsp70. Aipl1 also associates with the cell cycle regulator NUB1. It is thought that Aipl1 cooperates with Hsp70 but not Hsp90 to suppress the formation of NUB1 inclusions, and these interactions are necessary in the normal photoreceptor maturation, as mutations that lead to LCA also compromise the interactions with the Hsp chaperones. At least three isoforms of Aipl1 are known to exist.

Supplier: Rockland Immunochemical

AIMP1 (Endothelial monocyte-activating polypeptide II, EMAP2) is a proinflammatory cytokine for monocytes and granulocytes (1). It is specifically induced by apoptosis and is involved in the control of angiogenesis, inflammation, and wound healing (1,2). AIMP1 was identified as one of three auxiliary factors of the mammalian aminoacyl tRNA synthetase (ARS) complex. It binds and facilitates the catalytic reaction of arginyl-tRNA synthetase (2,3). Recent studies show that CD23 plays an essential role in the AIMP1-induced immune response and might be a target in the treatment of inflammatory diseases (4).