You searched for: Agarose

Supplier: VWR

A low-melting agarose that melts between 62 to 68 °C and remains liquid for several hours at 37 °C. Ideal for purification of macromolecules from agarose slices and for in-gel enzymatic manipulations.

Supplier: VWR

Fine white powder.

Supplier: MP Biomedicals

Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae.
Agarose is useful in separation of nucleic acids electrophoretically because agarose gels have larger pore sizes than cross linked acrylamide gels at low concentrations and in chromatographic separations by either beading the agarose or cross-linking it. Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions. Agarose was used to prepare 0.5% agarose gel for Southern blot analysis of the DNA extracted from primate muscles.
The following is a list of properties associated with our agaroses: Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present. Gel strength - the force that must be applied to a gel to cause it to fracture. Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Supplier: MP Biomedicals

Standard agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae with an Electroendoosmosis (EEO) value of 0.12 making it an essential component in chemical separation techniques.
Standard agarose is a very low electroendosmosis agarose (EEO) which is recommended for sharp resolution of nucleic acid fragments greater than 400 bp and up to 8000 bp. This Standard Agarose may be used at concentrations between 0.8 % and 2 %. Southern and Northern transfers can be performed efficiently from them.

Supplier: Cytiva

Agarose NA has been prepared to give the optimal combination of these properties: Reliable separation of nucleic acids; low inhibition of enzyme activity on recovered material; and high gel strength.

Supplier: Cytiva

Agarose with low electroendosmosis (EEO) is recommended for most DNA/RNA applications.

Supplier: Thermo Scientific Chemicals

Agarose for electrophoresis low electroendosmosis (EEO) (Mr <= 0.10)

Supplier: Thermo Scientific Chemicals

Supplier: MP Biomedicals

Agarose for electrophoresis

Supplier: Thermo Scientific Chemicals

Agarose for electrophoresis medium-low electroendosmosis (EEO) (Mr = 0.12)

Supplier: MP Biomedicals

Agarose is a natural polysaccharide isolated from agar or agar-bearing marine algae. Its physical and chemical properties are particularly suited for use as a gel medium for diffusion and electrokinetic movement of biomolecules. Agarose is essentially biologically inert and forms relatively clear, transparent gels. Agarose based gels offer several advantages: High gel strength permits use of low concentrations. The macroporous nature of the gels allows rapid diffusion of high molecular weight molecules. Agarose is Non-toxic. Agarose gels are thermally reversible facilitating sample recovery. Staining and destaining may be done rapidly with minimal background. Because agarose forms macroporous gels that are electrically nonionic, it is an excellent medium for Isoelectric Focusing (IEF). A selection of different formulated agaroses with specific qualities for unique applications are available from MP.
Multipurpose Agarose is a versatile high gel strength agarose, especially designed for a wide range of molecular biology techniques. It is used for resolution of fragments over a large size range from 200 bp up to 50 kb by conventional electrophoresis. It is suitable for use in pulsed field gel electrophoresis and for blotting assays.
High gel strength agarose, especially designed for a wide range DNA. Versatile – separate fragments from 200 bp up to 50 kb by conventional electrophoresis. Concentrations between 0.4% and 6%. Multipurpose Agarose features the highest gel strength available, allowing 0.4 - 0.5% agarose gels. Significantly decreases electrophoresis times. Improves separation of very large fragments. Dissolves easily in a microwave.

Supplier: MP Biomedicals

Agarose is a linear polymer consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marinealgae.

Supplier: Thermo Scientific Chemicals

Supplier: Thermo Scientific Chemicals

Supplier: MP Biomedicals

Agarose High Resolution is an intermediate melting agarose with approximately twice the resolution capability of routine agarose. It is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae. It is ideal for the resolution of small DNA fragments in the range 20 bp to 2000 bp.
Agarose high resolution is an intermediate melting and gelling point agarose which exhibits superior sieving characteristics. It is recommended for analytical gels of DNA fragments under 1000 bp and is ideal for small DNA fragments down to 20 bp can be separated on 6 % gels. It resolves fragments differing in size by only 2 bp. Its use avoids the need for PAGE Southern and Northern transfers can be achieved efficiently from them at concentrations up to 2%. DNA fragments can be purified with high efficiency from this agarose gels up to 4% by using either a purification kit or an electroelution system.

Supplier: MP Biomedicals

Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae. It is a linear polymer consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. It is specially prepared to allow recovery of active DNA after separation, with minimal inhibition of restriction and ligation enzymes. Suitable for both analytical and preparative electrophoresis procedures.

Supplier: Thermo Scientific Chemicals

Agarose, powder, pure for biochemistry (Mr = - 0.17)

Supplier: Thermo Scientific Chemicals

5G

Supplier: Thermo Scientific Chemicals

1000ml CAS: 62610-50-8, MDL: MFCD00081294

Supplier: Thermo Scientific Chemicals

1000ml CAS: 61970-08-9, MDL: MFCD00081294

Supplier: Thermo Scientific Chemicals

Agarose for electrophoresis, for electrophoresis medium electroendosmosis (EEO) (Mr = 0.16 - 0.19)

Supplier: MilliporeSigma

Low melting point. Preparative agarose melts at 65°C and remains liquid at 37°C for several hours. Allows for digestions and ligations in melted gel and easy recovery from gel slices. DNase-, RNase-, and protease-free.

Supplier: RPI

For DNA/RNA fragments > 1000bp

Supplier: MilliporeSigma

White solid. Highly purified nonionic fraction of agar. Forms gel with superior mechanical qualities at very low concentrations. Can be mounted on microscope slides or transparent plastic.

Supplier: MilliporeSigma

Suitable for a wide range of nucleic acid and protein gel applications. Of average gel strength and standard melting and gelling ranges. Low EEO for shorter electrophoretic runs. Molecular biology grade.

Supplier: RPI

For DNA/RNA fragments from 200-800bp.

Supplier: RPI

For DNA/RNA fragments > 1000 bp.

Supplier: G-Biosciences

G-Biosciences offers several different biotechnology-grade agarose powders. Agarose II has a low melting and gelling temperature compared to standard agaroses. The low melting temperature allows for the recovery of undamaged nucleic acids below the denaturation temperature. The low gelling temperature ensures that the agarose will be in a liquid state at a temperature range where in-gel manipulations can be performed without prior extraction from the agarose slice.

Supplier: Thermo Scientific Chemicals

Agarose, Electrophoresis Grade

Supplier: Thermo Scientific Chemicals

Agarose, low EEO