You searched for: Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

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NP-40 lysis buffer, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Triton® X-100 lysis buffer, pH 7.4, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Red Blood Cell Lysis Solution (CLB), 200ml, Promega

Supplier: Promega Corporation

Component of the SV Total RNA Isolation and PureYield RNA Midiprep Systems.

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Monarch® Precipitation Enhancer, New England Biolabs

Supplier: New England Biolabs (NEB)

The Monarch® precipitation enhancer solution, supplied as a component of the Monarch® HMW DNA extraction kit for cells and blood, is a salt-based solution that facilitates the precipitation of DNA onto the Monarch® DNA capture beads during HMW DNA extraction from cells and blood.

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Passive Lysis 5X Buffer, Promega

Supplier: Promega Corporation

Promotes rapid lysis of cultured mammalian cells without the need to scrape adherent cells or freeze-thaw. Lytic reagent of choice when processing cells for quantitation of firefly and Renilla luciferase activities using the Promega Dual-Luciferase® Reporter Assay (#PAE1910).

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Luna Cell Ready Lysis Module, New England Biolabs

Supplier: New England Biolabs (NEB)

Cell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. Traditionally, RNA is extracted and purified from treated cells via column-based or chemical methods. The Luna Cell Ready Lysis Module is a fast, convenient, and sensitive alternative for evaluating RNA expression levels without performing traditional RNA extraction steps.

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Phosphate buffered RIPA, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Cell Lysis Solution, Promega®

Supplier: Promega Corporation

Cell Lysis Solution is a component of the Wizard MagneSil, Wizard Plus and Wizard SV 96 and SV 9600 Plasmid DNA Purification Systems and the PureYield Plasmid Midiprep System.

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RIPA buffer with Triton® X-100 (5X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Succinate, 0.2M buffer soln., pH 5.0, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Triton® X-100 lysis buffer with glycerol (2X), pH 7.4, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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CTAB Buffer, Promega

Supplier: Promega Corporation

Lysis buffer for use with the Maxwell® RSC PureFood GMO and Authentication Kit.

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ToxiLight® 100% Lysis Control Set

Supplier: Lonza

The ToxiLight® 100% lysis reagent set is intended for use with the ToxiLight® non-destructive cytotoxicity assay (sold seperately) and is designed to give the user a 100% lysis or total adenylate kinase control.

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Lysis Buffer, Promega

Supplier: Promega Corporation

Lysis Buffer, Blood, is a component of the MagneSil Blood Genomic, Max Yield System (Cat.# MD1360), and MagneSil ONE, Fixed Yield Blood Genomic System (Cat.# MD1370).

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Monarch® Protein Separation Solution, New England Biolabs

Supplier: New England Biolabs (NEB)

The Monarch® Protein separation solution, supplied as a component of the Monarch HMW DNA extraction kit for tissue, is a salt-based solution that is optimized to ensure proteins in tissue and bacteria samples are easily removed during the phase separation of the Monarch HMW DNA extraction workflow.

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Monarch® gDNA Cell Lysis Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

The Monarch® gDNA Cell Lysis Buffer is a component of the Monarch® Genomic DNA Purification Kit (NEB #T3010), which can be used to purify up to 30 µg genomic DNA from a variety of sample types.

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Monarch® gDNA Tissue Lysis Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

The Monarch® gDNA Tissue Lysis Buffer is a component of the Monarch® Genomic DNA Purification Kit (NEB #T3010), which can be used to purify up to 30 µg genomic DNA from a variety of sample types.

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NEBExpress® E. coli Lysis Reagent, New England Biolabs

Supplier: New England Biolabs (NEB)

NEBExpress® E. coli Lysis Reagent is a chemical lysis solution composed of a proprietary mix of non-ionic and zwitterionic detergents and Tris-based buffer. It allows disruption of E. coli cells without denaturing soluble proteins.

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Denaturing lysis buffer, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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2D-Xtract™, G-Biosciences

Supplier: G-Biosciences

2D-Xtract™ is a dry, urea-based and ready-to-use protein solubilization buffer.

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Monarch® RNA Wash Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

Need more RNA Wash Buffer? No problem. Monarch® kit components are all available for purchase separately.

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Monarch® RNA Lysis Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

Need more RNA Lysis Buffer? No problem. Monarch® Kit components are all available for purchase separately.

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RNAgents® Denaturing Solution, Promega

Supplier: Promega Corporation

RNAgents Denaturing Solution lyses cells or tissue under conditions that rapidly inhibit ribonucleases.

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Lysis Buffer A, Promega

Supplier: Promega Corporation

Components of the Wizard Magnetic DNA Purification System for Food, which is intended for isolating genomic DNA from a variety of food samples including: corn seeds, corn meal, soy beans, soy flour, soy milk, corn oil, soybean oil, canola oil, etc.