Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

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VWR® Hypotonic Lysis Buffer

Supplier: VWR

Cell lysis in detergent-free Hypotonic Lysis buffer and the subsequent isolation of the crude cytoplasmic fraction can be completed in less than 30 minutes

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J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

J.T.Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are biodegradable and octyl phenol ethoxylate free.

Sustainable Options Available

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J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

Sustainable Options Available

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g-DNA Wash Buffer, Zymo Research

Supplier: Zymo Research

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Lysis Buffer 7X (Blue), Zymo Research

Supplier: Zymo Research

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ZymoBIOMICS Lysis Solution, Zymo Research

Supplier: Zymo Research

ZymoBIOMICS Lysis Solution is designed to be used with the ZymoBIOMICS DNA extraction kits. Used in combination with the ZR BashingBead Lysis Tubes, it assists with microbial lysis. It is certified to be low-bioburden to ensure minimal background contaminants for low abundance microbiome samples.