You searched for: Extraction Buffers

Supplier: Abcam

Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types

Supplier: Abcam

Abcam's Extraction Enhancer Buffer 50X is formulated for the preparation of cell or tissues extracts for use in immunoassays.

Supplier: VWR

Cell lysis in detergent-free Hypotonic Lysis buffer and the subsequent isolation of the crude cytoplasmic fraction can be completed in less than 30 minutes

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

J.T.Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are biodegradable and octyl phenol ethoxylate free.

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

Supplier: Rockland Immunochemical

ProXtract B™ is ready-to-use as a working 1X solution and requires no further dilution.

Supplier: Advanced Instruments

The fluorophos cheese extraction buffer is used to extract alkaline phosphatase from cheese products.

Supplier: G-Biosciences

G-Biosciences' RIPA Lysis and Extraction Buffer is a complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells

Supplier: G-Biosciences

One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers

Supplier: Thermo Scientific Chemicals

Liquid

Supplier: Enzo Life Sciences

Concentrated lysis buffer for preparation of cell and tissue extracts.

Supplier: Enzo Life Sciences

Concentrated lysis buffer for preparation of cell and tissue extracts.

Supplier: Omega Bio-Tek

Binding Buffer (XP2), Omega Bio-Tek

Supplier: G-Biosciences

Lysis and extraction of biologically active proteins from cellular and tissue samples is the first critical step for biochemical analysis

Supplier: Abcam

10X RIPA lysis buffer (pH 7.5) is a means of cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells. - Cited in over 180 publications - Suitable for immunoprecipitation (IP), ELISA, SDS-PAGE, western blot - Effectively extracts cytoplasmic, nuclear and membrane proteins

Supplier: Enzo Life Sciences

Supplier: Abcam

This product is manufactured by BioVision, an Abcam company and was previously called 1067 Cell Lysis Buffer.

Supplier: Rockland Immunochemical

SDS, sodium dodecyl sulfate is an anionic surfactant.

Supplier: Abcam

Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.

Supplier: Invitrogen

The Pierce™ kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

Supplier: Zymo Research

Supplier: Zymo Research

Supplier: Zymo Research

Supplier: MP Biomedicals

BioPulverizer™ System II contains 10x2 mL tubes of each of our specialized Lysing Matrices.

Supplier: Zymo Research

Supplier: Enzo Life Sciences

100 mL

Supplier: Rockland Immunochemical

RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells

Supplier: Agilent Technologies

Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.

Supplier: New England Biolabs (NEB)

New England Biolabs supplies a unique 5X reaction buffer and supplemental reagents for use with Thermo Scientific® Phusion High-Fidelity DNA Polymerase and associated products, all of which are manufactured and quality controlled at New England Biolabs.

Supplier: Beckman Coulter

Whole blood assays are our best approximation of physiological conditions in research flow cytometry. Density gradient leukocyte isolation or bulk lysing procedures are common to remove red blood cells, but decompose the native material prior to antigen detection. Common lysing buffers avoid this decomposition and eliminate the gradient-related preanalytical workload, but also increase sample volume beyond the single-well capacity of many plate formats, limiting throughput and automation options.