Blocking Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

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BSA blocking buffer, 5% in TBS, with 0.05% Tween-20, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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BSA blocking buffer, 5% in PBS, with 0.05% Tween-20, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Blocking Ragents, Denhardt's Solution (50X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Denhardt's solution is used to reduce non-specific binding of detection reagents to the northern and southern blot membranes.

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BSA Blocking Buffer, 5% in PBS, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Protein Block, Abcam

Supplier: Abcam

Protein block is a serum-free blocking buffer designed to reduce non-specific background staining in IHC, ELISA, and western blot (WB). - Cited in over 90 publications - No need to match species for secondary antibodies, reducing animal serum handling - Ready-to-use – no mixing or dilution required

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Casein Blocking Buffer, 3% in PBS, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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BLOTTO Antifoam in PBS, with 0.02% Sodium Azide, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Blocking reagent for immunohistochemistry experiments.

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Antibody Blocker/Diluent, Enzo Life Sciences

Supplier: Enzo Life Sciences

Ready-to-use antibody blocking buffer and diluent, for use with POLYVIEW® Plus detection reagents.

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Pierce™ Blocking Buffer, Protein Free, Thermo Scientific

Supplier: Thermo Scientific

These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.

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Pierce™ Fast Blocking Buffe, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce Fast Blocking Buffer effectively and reliably blocks Western blots in just five minutes to provide low-background results and speed up traditional blotting protocols.