Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk to reduce the background. Nonfat dried milk is often preferred as it is inexpensive and widely available. However, milk proteins are not compatible with all detection labels, so care must be taken to choose the appropriate blocking solution.

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Milk Diluent/Blocking Concentrate Kit, KPL Immunoassay Kits, Seracare

Supplier: KPL

Convenient antibody diluent/blocking solution concentrate containing 2% nonfat milk in a borate buffer.

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SuperBlock™ (PBS) Blocking Buffer, Thermo Scientific

Supplier: Invitrogen

SuperBlock™ Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

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SuperBlock™ T20 (TBS) Blocking Buffer, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific SuperBlock™ Blocking Buffer is optimised solutions in PBS or TBS containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western blot analysis.

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Pierce™ Nucleic Acid Detection Blocking Buffer, Thermo Scientific

Supplier: Invitrogen

Nucleic Acid Detection Blocking Buffer is intended for use with the Thermo Scientific Chemiluminescent Nucleic Acid Detection Module Kit, of which it is also a component

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Dry Milk Powder

Supplier: Biotium

Milk is commonly used in blocking buffers minimize background noise in immunodetection procedures such as western blotting and immunostaining.

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Pierce™ SuperBlock™ Dry Blend Blocking Buffer, Thermo Scientific

Supplier: Invitrogen

SuperBlock (TBS) Blocking Buffer is an optimised TBS solution containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western Blot Analysis.

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Aquatic Block™ PBS

Supplier: Arlington Scientific

A non-mammalian blocking reagent supplied in PBS for diagnostic systems involving membrane or other solid phase material.

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Aqua Block™ Tris

Supplier: Arlington Scientific

A non-mammalian blocking reagent supplied in tris for diagnostic systems involving membrane or other solid phase material.

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BLOK™ Casein, 1% Casein Protein Blocking Solution, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' BLOK™ Casein is a purified protein, used for blocking free binding sites in western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.

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Pierce™ Fish Serum Blocking Buffe, Thermo Scientific

Supplier: Invitrogen

This Fish Serum Blocking Buffer is Steelhead Salmon Serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.

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CellPro™ BSA-Blocking Buffer, 3% + Tween-20 0.05% in TBS

Supplier: Alkali Scientific

Enhance protein detection with CellPro™ BSA-Blocking Buffer, featuring 3% BSA and 0.05% Tween-20 in TBS for effective blocking.

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Pierce™ StartingBlock™ (PBS) Blocking Buffer, Thermo Scientific

Supplier: Invitrogen

These StartingBlock™ blocking buffers are PBS and TBS formulations of a proprietary protein that provides exceptional performance and broad compatibility with membrane (Western blot) and 96-well plate (ELISA) assays.

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CellPro™ Western Blot Blocking Buffer, Sterile Filtered

Supplier: Alkali Scientific

Achieve reliable protein blocking in western blotting with CellPro™ western blot blocking buffer, provided sterile filtered for consistency.

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Pierce™ Clear Milk Blocking Buffer (10X), Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce Clear Milk Blocking Buffer (10X) is a long shelf-life, high performance replacement for homemade milk blocking buffers in Western blotting applications.

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Pierce™ Blocking Buffer, Protein Free, Thermo Scientific

Supplier: Invitrogen

These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.

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Blocking Solution, 10% BSA, KPL Immunoassays

Supplier: KPL

10% BSA Diluent/Blocking Solution minimizes non-specific binding and background

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Blocking Reagents, BLOTTO and Tween 20 in PBS, with 0.02% Sodium Azide, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Blocking reagent for immunohistochemistry experiments.

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Balance Blocker™ PBS (with Surfactant)

Supplier: Arlington Scientific

A universal blocking reagent supplied in PBS for diagnostic and research systems.

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Aquatic Block™ Serum-Free PBS (with Surfactant)

Supplier: Arlington Scientific

A non-mammalian blocking reagent supplied in PBS for diagnostic systems involving membrane or other solid phase material.

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BLOK™ BSA, 10% BSA Protein Blocking Solution, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' BLOK™ BSA is a 10% BSA protein blocking solution suitable for blocking Western blots, ELISA and dot Blots

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Aquatic Block™ Serum-Free Tris (with Surfactant)

Supplier: Arlington Scientific

A proprietary blocking reagent supplied in tris for diagnostic systems involving membrane or other solid phase material.

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BLOK™ BLOTTO, 5% Modified Milk Protein Blocking Solution, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' BLOK™ BLOTTO is a pre-formulated, convenient, ready-to-use blocking solution

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Aurion Blocking Solutions, Electron Microscopy Sciences

Supplier: Electron Microscopy Sciences

Aurion blocking solutions effectively block sticky surfaces by multi-point hydrophobic and charge-based interactions. The blocking solutions are specifically designed to meet the characteristics of each type of secondary conjugate.