Cloning Buffers
In molecular biology, cloning buffer solutions containing TRIS, acetic acid, EDTA, or more are used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. Various buffer formulations are available with different base ingredients in the mixture. These different mixtures have varied buffer capacities and run times. Buffer solutions serve to protect samples against pH changes while the sample is undergoing a wide variety of chemical applications.
EDTA solution, 0.5M, pH 8.0
Supplier: VWR International
N.I.S.T traceable to SRM 919.
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VWR® EDTA 0.5M, Biotechnology Grade
Supplier: VWR
Cloning Buffer, EDTA, 0.5M Solution, pH 8.0
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VWR® Sodium Acetate, 3M Solution, pH 5.2, Biotechnology Grade
Supplier: VWR
Cloning Buffer, Sodium acetate solution 3M, pH 5.2
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VWR® EDTA 0.5M, Biotechnology Grade
Supplier: VWR
Cloning Buffer, EDTA, 0.5M Solution, pH 8.0
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EDTA (0.5 M), pH 8.0, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents, inorganic salts, and chelators.
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TE (Tris EDTA) Buffers, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation.
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NEBuffer Set (EcoRI, DpnII), New England Biolabs
Supplier: New England Biolabs (NEB)
New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity.
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NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™), New England Biolabs
Supplier: New England Biolabs (NEB)
New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. This set contains NEBuffers that contain recombinant Albumin.
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NEBuffer 4 , New England Biolabs
Supplier: New England Biolabs (NEB)
New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity
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StickTogether DNA Ligase Buffer, New England Biolabs
Supplier: New England Biolabs (NEB)
StickTogether Buffer is supplied with T3, T7, Hi-T4 and Salt-T4 DNA ligases.
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NEBuffer 2 , New England Biolabs
Supplier: New England Biolabs (NEB)
New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity
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Low-EDTA TE (1X), pH 8.0, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
Low-EDTA 1X, pH 8.0 is molecular biology grade TE buffer used to store DNA and RNA. EDTA chelates Mg2+ and other divalent metals ions - (inhibits DNAse and RNAse to suppress DNA and RNA degradation).
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NEBuffer 3 , New England Biolabs
Supplier: New England Biolabs (NEB)
New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity
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Tris(hydroxymethyl)aminomethane hydrochloride, 1M soln., pH 7,4, RNase free, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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#: 21905-026
Supplier: Kimberly-Clark
#: 32934-080
Supplier: Kimberly-Clark
#: 89039-656
Supplier: VWR International