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250 results for Vectors, Plasmids and Libraries

You searched for: Vectors, Plasmids and Libraries

Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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pF3A WG (BYDV) Flexi Vector, 20 µg, Promega

pF3A WG (BYDV) Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest.

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pFC30A His6HaloTag T7 Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.

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pGL4.32[luc2P/NF-kappa-B-RE/Hygro] Vector, 20 µg, Promega

pGL4.32[luc2P/NF-kappa-B-RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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pGloSensor-22F cAMP Plasmid, 20 µg, Promega

Supplier: Promega Corporation

The GloSensor cAMP Assay is based on a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.

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pRL-TK Vector, 20 µg, Promega

pRL-TK Vector, 20 µg, Promega

Supplier: Promega Corporation

The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.

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pNL2.3[secNluc/Hygro] Vector, 20 µg, Promega

pNL2.3[secNluc/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

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West Nile Virus (WNV) Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

West Nile Virus (WNV) Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

Supplier: ReVacc Scientific

Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of West Nile Virus (WNV) (GenBank: DQ211652.1, NY99 strain) and a Renilla luciferase reporter. As the wild typed WNV is handled under BSL-2 precautions, this WNV RVP can be handled using BSL-2 containment practices.

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Lentiviral Control Vectors, Cell Biolabs

Lentiviral Control Vectors, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' Lentiviral Control Plasmids are constructs containing one of a variety of reporter genes

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pMXs Retroviral Expression Vectors, Cell Biolabs

pMXs Retroviral Expression Vectors, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' pMXs vectors are suitable for making retrovirus for general gene expression in various cells

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pMYs Retroviral Expression Vectors, Cell Biolabs

pMYs Retroviral Expression Vectors, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' pMYs vectors are suitable for making retrovirus for gene expression in hematopoietic stem cells

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N OLLAS Vector, Novus Biologicals (NBA1-07083)

N OLLAS Vector, Novus Biologicals (NBA1-07083)

Supplier: Novus Biologicals

The pCMV-N-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with an N-terminal OLLAS tag. Using the N-OLLAS vector the protein will be expressed in the cytoplasm. We recommend inserting your protein of interest into the vector in frame using the Hind III and Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.

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Pierce™ Green Renilla Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Pierce™ Green Renilla Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Supplier: Invitrogen

Green Renilla Luc vectors contain a gene cloned from the sea pansy, Renilla reniformis

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AAV-1 Helper Free Expression Complete Systems, Cell Biolabs

AAV-1 Helper Free Expression Complete Systems, Cell Biolabs

Supplier: Cell Biolabs

AAV-1 Helper Free Expression Complete Systems contain the following constructs needed to package your recombinant serotype-1 adeno-associated virus: an expression vector, pHelper plasmid, a serotype-specific Rep-Cap plasmid, and an optional GFP expression vector for making a control virus

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pMZs Retroviral Expression Vector, Cell Biolabs

pMZs Retroviral Expression Vector, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' pMZs vector is suitable for making retrovirus for general gene expression

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Pierce™ Cypridina Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Pierce™ Cypridina Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Supplier: Invitrogen

Cypridina Luc vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca

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pWZL Retroviral Expression Vector, Cell Biolabs

pWZL Retroviral Expression Vector, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' pWZL vector is suitable for making retrovirus for general gene expression in various cells

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Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Supplier: Invitrogen

Gaussia Dura Luc vectors contain a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20 kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

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Pantropic Lentiviral Expression Complete Systems, Cell Biolabs

Pantropic Lentiviral Expression Complete Systems, Cell Biolabs

Supplier: Cell Biolabs

ViraSafe™ Pantropic Lentiviral Expression Systems contain an expression vector plus all the packaging plasmids necessary to make recombinant lentivirus

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AAV-6 Helper Free Expression Complete Systems, Cell Biolabs

AAV-6 Helper Free Expression Complete Systems, Cell Biolabs

Supplier: Cell Biolabs

AAV-6 Helper Free Expression Complete Systems contain the following constructs needed to package your recombinant serotype-6 adeno-associated virus: an expression vector, pHelper plasmid, a serotype-specific Rep-Cap plasmid, and an optional GFP expression vector for making a control virus

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AAV Rep-Cap Vectors, Cell Biolabs

AAV Rep-Cap Vectors, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' AAV Rep-Cap plasmids work with our AAV Helper Free Systems to produce recombinant AAV more safely and easily, without the need for a helper virus

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GFP Retroviral Vectors, Cell Biolabs

GFP Retroviral Vectors, Cell Biolabs

Supplier: Cell Biolabs

PMXs-GFP/Puro Retroviral Vector, Efficient tools for delivering heritable genes into the genome of dividing cells, Contains: Ampicillin-resistance gene, MMLV LTRs, package signal and GFP-Puro fusion insert, Storage: -20 deg C, Size and Concentrati

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AAV-2 Helper Free Expression Complete Systems, Cell Biolabs

AAV-2 Helper Free Expression Complete Systems, Cell Biolabs

Supplier: Cell Biolabs

AAV-2 Helper Free Expression Complete Systems contain the following constructs needed to package your recombinant serotype-2 adeno-associated virus: an expression vector, pHelper plasmid, a serotype-specific Rep-Cap plasmid, and an optional GFP expression vector for making a control virus

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Ecotropic Lentiviral Expression Complete Systems, Cell Biolabs

Ecotropic Lentiviral Expression Complete Systems, Cell Biolabs

Supplier: Cell Biolabs

ViraSafe™ Ecotropic Lentiviral Expression Systems contain an expression vector plus all the packaging plasmids necessary to make recombinant lentivirus

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AAV Helper Free Packaging Systems, Cell Biolabs

AAV Helper Free Packaging Systems, Cell Biolabs

Supplier: Cell Biolabs

AAV Helper Free Packaging Systems contain the following constructs needed to package your recombinant adeno-associated virus along with your own expression vector: pHelper plasmid, a serotype-specific Rep-Cap plasmid, and an optional GFP expression vector for making a control virus

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AAV-4 Helper Free Expression Complete Systems, Cell Biolabs

AAV-4 Helper Free Expression Complete Systems, Cell Biolabs

Supplier: Cell Biolabs

AAV-4 Helper Free Expression Complete Systems contain the following constructs needed to package your recombinant serotype-4 adeno-associated virus: an expression vector, pHelper plasmid, a serotype-specific Rep-Cap plasmid, and an optional GFP expression vector for making a control virus

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Envelope Vectors, Cell Biolabs

Envelope Vectors, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' Envelope Vectors each contain a different envelope gene for pseudotyping recombinant lentivirus or retrovirus

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pBABE Retroviral Expression Vectors, Cell Biolabs

pBABE Retroviral Expression Vectors, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' pBABE vectors are suitable for making retrovirus for general gene expression in various cells

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Pierce™ Red Firefly Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Pierce™ Red Firefly Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Supplier: Invitrogen

Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.

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pMCs Retroviral Expression Vectors, Cell Biolabs

pMCs Retroviral Expression Vectors, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' pMYs vectors are suitable for making retrovirus for gene expression in embryonic stem cells

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AAV-5 Helper Free Expression Complete Systems, Cell Biolabs

AAV-5 Helper Free Expression Complete Systems, Cell Biolabs

Supplier: Cell Biolabs

AAV-5 Helper Free Expression Complete Systems contain the following constructs needed to package your recombinant serotype-5 adeno-associated virus: an expression vector, pHelper plasmid, a serotype-specific Rep-Cap plasmid, and an optional GFP expression vector for making a control virus

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