Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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AAV-5 Helper Free Expression Complete Systems, Cell Biolabs

Supplier: Cell Biolabs

AAV-5 Helper Free Expression Complete Systems contain the following constructs needed to package your recombinant serotype-5 adeno-associated virus: an expression vector, pHelper plasmid, a serotype-specific Rep-Cap plasmid, and an optional GFP expression vector for making a control virus

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AAV Expression Vectors, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' AAV Expression Vectors are designed to work with any AAV packaging system including the AAV Helper Free Packaging Systems

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pMCs Retroviral Expression Vectors, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' pMYs vectors are suitable for making retrovirus for gene expression in embryonic stem cells

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SD OLLAS/DYKDDDDK Vector, Novus Biologicals (NBA1-07085)

Supplier: Novus Biologicals

The pCMV-NS-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with both an N-terminal OLLAS tag and a DYKDDDDK tag. Using this vector the protein will be secreted from the cell due to the inclusion of a signal peptide. We recommend inserting your protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.

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Pierce™ Red Firefly Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Supplier: Invitrogen

Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.

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AAV-1 Helper Free Expression Complete Systems, Cell Biolabs

Supplier: Cell Biolabs

AAV-1 Helper Free Expression Complete Systems contain the following constructs needed to package your recombinant serotype-1 adeno-associated virus: an expression vector, pHelper plasmid, a serotype-specific Rep-Cap plasmid, and an optional GFP expression vector for making a control virus