Vectors, Plasmids and Libraries

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Musoke (GenBank: DQ217792.1).

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA. The S has 18-aa cytoplasmic tail truncation for optimal infection.

Supplier: Promega Corporation

pBR322 Vector.

Supplier: Promega Corporation

These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) (Wuhan strain or Wild type strain), with 18-aa cytoplasmic tail truncation for optimal infection.

Supplier: Promega Corporation

These multiple cloning site vectors are used for inducible expression of HaloTag-polyhistidine fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.

Supplier: Promega Corporation

The Monster Green Fluorescent Protein encoded by the hMGFP gene is an ideal fluorescent reporter, providing high-level fluorescence and reducing cytotoxicity.

Supplier: Promega Corporation

The pGL4.10[luc2], pGL4.11[luc2P] and pGL4.12[luc2CP] Vectors are promoterless reporter vectors optimized for expression in mammalian cells. The different luc2 genes code for luciferase proteins with different stabilities.

Supplier: Promega Corporation

The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.

Supplier: Promega Corporation

The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

Supplier: New England Biolabs (NEB)

The single-stranded viral DNA is isolated from M13mp18

Supplier: Promega Corporation

The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

Supplier: Vector Biolabs

POU (Pit, Oct, Unc) homeodomain proteins are transcriptional regulators that function in various developmental processes such as cell division, differentiation, specification and survival of specific cell types and participation in the determination of cell fate. POU domain factors are transcriptional regulators characterized by a bipartite DNA binding domain, which consists of two highly conserved regions tethered by a variable linker of 14 to 16 amino acids. The versatility of POU protein operation is additionally conferred at the dimerization level. Transcription factors containing the POU homeodomain are important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. There are five classes of POU Domain Family proteins. Pit-1 is a member of class I; Bob 1, Skn-1a/i, Oct-1 and Oct-2 are members of class II; Oct-6, Brn-1 and Brn-2 are members of class III; Brn-3 is a member of class IV; and Oct-3 (Oct-4) is a member of class V.

Supplier: Vector Biolabs

Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals. This AAV1 stock expresses an improved Cre (iCre) along with eGFP under a control of the same CMV promoter. The Cre and eGFP was separated by a 2A peptides.

Supplier: Vector Biolabs

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells.