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250 results for Vectors, Plasmids and Libraries

You searched for: Vectors, Plasmids and Libraries

Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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pGL4.73[hRluc/SV40] Vector, 20 µg, Promega

pGL4.73[hRluc/SV40] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.73[hRluc/SV40], pGL4.74[hRluc/TK] and pGL4.75[hRluc/CMV] Vectors are used as the control reporter for dual-luciferase assays. Select the appropriate promoter or test all three in your experimental system.

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pGL4.26[luc2/minP/Hygro] Vector, 20 µg, Promega

pGL4.26[luc2/minP/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.26[luc2/minP/Hygro], pGL4.27[luc2P/minP/Hygro] and pGL4.28[luc2CP/minP/Hygro] Vectors are used to clone a response element of interest upstream of the minimal promoter and firefly luciferase gene with and without destabilization sequences.

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VSV-Pseudovirus_SARS-CoV-2 Delta Luciferase, ReVacc Scientific

VSV-Pseudovirus_SARS-CoV-2 Delta Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of B.1.617.2 (Delta strain), and 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.75 Strain Spike with Luciferase Reporter

VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.75 Strain Spike with Luciferase Reporter

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA.2.75. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.86 Luciferase

VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.86 Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA. The S has 18-aa cytoplasmic tail truncation for optimal infection.

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pNLF1-HIF1A [CMV/neo] Vector, 1 each, Promega

pNLF1-HIF1A [CMV/neo] Vector, 1 each, Promega

Supplier: Promega Corporation

NanoLuc Stability Sensors are ready-to-use vector systems that utilize the advantages of the NanoLuc luciferase reporter for stability studies of two key signaling proteins, HIF1A and NRF2, providing a way to directly measure the primary signaling event.

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pGL4.18[luc2P/Neo] Vector, 20 µg, Promega

pGL4.18[luc2P/Neo] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.17[luc2/Neo] Vector, pGL4.18[luc2P/Neo] and pGL4.19[luc2CP/Neo] Vectors are optimized for expression in mammalian cells. These vectors offer standard and destablized luciferase with neomycin for stable selection.

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HaloTag Flexi Vectors-CMV Deletion Series Sample Pack, 9 x 2microg, Promega

HaloTag Flexi Vectors-CMV Deletion Series Sample Pack, 9 x 2microg, Promega

Supplier: Promega Corporation

Vectors in the sample pack are configured to append the HaloTag protein to either the carboxy- (pFC vectors) or amino-terminus (pFN vectors) of the protein fusion partner and provide constitutive protein expression in mammalian cells.

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pFN31A Nluc CMV-Hygro Flexi Vector, 20 µg, Promega

pFN31A Nluc CMV-Hygro Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

The pFN31 and pFC32 Vectors generate N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. Use pFN31A/K Nluc CMV-Hygro Flexi Vectors for N-terminal fusions and pFC32A/K Nluc CMV-Hygro Flexi Vectors for C-terminal fusions.

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M13mp18 Single-Stranded DNA, New England Biolabs

M13mp18 Single-Stranded DNA, New England Biolabs

Supplier: New England Biolabs (NEB)

The single-stranded viral DNA is isolated from M13mp18

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Monster Green Fluorescent Protein phMGFP Vector, 20 µg, Promega

Monster Green Fluorescent Protein phMGFP Vector, 20 µg, Promega

Supplier: Promega Corporation

The Monster Green Fluorescent Protein encoded by the hMGFP gene is an ideal fluorescent reporter, providing high-level fluorescence and reducing cytotoxicity.

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pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector, 20 µg, Promega

pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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pGL4.48[luc2P/SBE/Hygro] Vector, 20 µg, Promega

pGL4.48[luc2P/SBE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.

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pNL3.2.NF-kB-RE[NlucP/NF-kB-RE/Hygro] Vector, 20 µg, Promega

pNL3.2.NF-kB-RE[NlucP/NF-kB-RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

NanoLuc (Nluc) luciferase is a 19.1kDa luminescent reporter enzyme that is about 100-fold brighter than either firefly or Renilla luciferase. Use the pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] Vector to measure changes in the levels of NF-kappaB in cells.

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pNL1.3[secNluc] Vector, 20 µg, Promega

pNL1.3[secNluc] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

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VSV-Pseudotyped HMPV Fusion with Luciferase Reporter (NL/1/00/A1 strain)

VSV-Pseudotyped HMPV Fusion with Luciferase Reporter (NL/1/00/A1 strain)

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the full length fusion (F) protein of Human metapneumovirus (HMPV) isolate NL/1/00/A1 (294E). HMPV is a respiratory virus with fusion (F) protein as the sole target of neutralizing antibodies. As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices.

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pSNAPf Vector, New England Biolabs

pSNAPf Vector, New England Biolabs

Supplier: New England Biolabs (NEB)

pSNAPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of SNAP-tag® protein fusions in mammalian cells

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VSV-Pseudovirus_SARS-CoV-2 with Luciferase, ReVacc Scientific

VSV-Pseudovirus_SARS-CoV-2 with Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) (Wuhan strain or Wild type strain), with 18-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_Marburg Musoke Luciferase, ReVacc Scientific

VSV-Pseudovirus_Marburg Musoke Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Musoke (GenBank: DQ217792.1).

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pFC14A HaloTag CMV Flexi Vector, 20 µg, Promega

pFC14A HaloTag CMV Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Vectors designed for expression of C-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

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pNLCoI1[luc2-P2A-NlucP/Hygro] Vector, 20 µg, Promega

pNLCoI1[luc2-P2A-NlucP/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.

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pNL1.1.PGK[Nluc/PGK] Vector, 20 µg, Promega

pNL1.1.PGK[Nluc/PGK] Vector, 20 µg, Promega

Supplier: Promega Corporation

The promoter-driven NanoLuc (Nluc) control vectors can be used to co-transfect with experimental firefly luciferase vectors when using the Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System.

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pNLF1-C [CMV/Hygro] Vector, 20 µg, Promega

pNLF1-C [CMV/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The three pNLF1 Vectors create N- or C-terminal fusions of NanoLuc luciferase and your protein of interest using a multiple cloning site (MCS). Insert your protein on the N or C terminus of NanoLuc luciferase or the N terminus of secreted NanoLuc enzyme.

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pGL4.52[luc2P/STAT5RE/Hygro] Vector, 20 µg, Promega

pGL4.52[luc2P/STAT5RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.

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pGL4.19[luc2CP/Neo] Vector, 20 µg, Promega

pGL4.19[luc2CP/Neo] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.17[luc2/Neo] Vector, pGL4.18[luc2P/Neo] and pGL4.19[luc2CP/Neo] Vectors are optimized for expression in mammalian cells. These vectors offer standard and destablized luciferase with neomycin for stable selection.

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pGL4.17[luc2/Neo] Vector, 20 µg, Promega

pGL4.17[luc2/Neo] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.17[luc2/Neo] Vector, pGL4.18[luc2P/Neo] and pGL4.19[luc2CP/Neo] Vectors are optimized for expression in mammalian cells. These vectors offer standard and destablized luciferase with neomycin for stable selection.

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pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector, 20 µg, Promega

pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.

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pFN26A (BIND) hRluc-neo Flexi Vector, 20 µg, Promega

pFN26A (BIND) hRluc-neo Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.

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pGL4.34[luc2P/SRF-RE/Hygro] Vector, 20 µg, Promega

pGL4.34[luc2P/SRF-RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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HaloTag Control Vector, 20microg, Promega

HaloTag Control Vector, 20microg, Promega

Supplier: Promega Corporation

The HaloTag Mammalian Pull-Down Systems capture and purify intracellular protein complexes for correlative cellular localization and real-time imaging studies with the same genetic construct for further understanding of overall protein function.

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