Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
pETDuet™-1 DNA Bacterial Expression Vector, EMD MilliporeSigma
Supplier: MilliporeSigma
pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS), each of which is preceded by a T7 promoter, lac operator and ribosome binding sites.
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Novagen® pET-21a Vectors, MilliporeSigma
Supplier: MilliporeSigma
The pET-21a vectors carry an N-terminal T7 Tag® sequence plus an optional C-terminal His Tag® sequence.
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Novagen® pET-23a-d(+) Vectors, MilliporeSigma
Supplier: MilliporeSigma
The pET-23a-d(+) vectors carry an N-terminal T7 Tag® sequence plus an optional C-terminal His Tag® sequence.
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Novagen® pET-28b(+) DNA, MilliporeSigma
Supplier: MilliporeSigma
The pET-28a-c(+) vectors carry an N-terminal His Tag®/thrombin/T7 Tag® configuration plus an optional C-terminal His Tag sequence.
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pGL4.48[luc2P/SBE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.
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pFN29A His6HaloTag T7 Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.
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VSV-Pseudovirus_SARS-CoV-2 Delta Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of B.1.617.2 (Delta strain), and 18-aa cytoplasmic tail truncation for optimal infection.
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pRL-Null Vector, 20 µg, Promega
Supplier: Promega Corporation
The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.
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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.75 Strain Spike with Luciferase Reporter
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA.2.75. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.86 Luciferase
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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VSV-Pseudovirus_Marburg Musoke Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Musoke (GenBank: DQ217792.1).
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pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.
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pNL3.2.NF-kB-RE[NlucP/NF-kB-RE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
NanoLuc (Nluc) luciferase is a 19.1kDa luminescent reporter enzyme that is about 100-fold brighter than either firefly or Renilla luciferase. Use the pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] Vector to measure changes in the levels of NF-kappaB in cells.
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Monster Green Fluorescent Protein phMGFP Vector, 20 µg, Promega
Supplier: Promega Corporation
The Monster Green Fluorescent Protein encoded by the hMGFP gene is an ideal fluorescent reporter, providing high-level fluorescence and reducing cytotoxicity.
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HaloTag Flexi Vectors-CMV Deletion Series Sample Pack, 9 x 2microg, Promega
Supplier: Promega Corporation
Vectors in the sample pack are configured to append the HaloTag protein to either the carboxy- (pFC vectors) or amino-terminus (pFN vectors) of the protein fusion partner and provide constitutive protein expression in mammalian cells.
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