You searched for: Vectors, Plasmids and Libraries

Supplier: Avantor

These plasmids allow you to perform bacterial transformations, conferring new antibiotic resistance, as a visual way of demonstrating gene transfer.

Supplier: Cytiva

Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.

Supplier: Agilent Technologies

The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.

Supplier: Cytiva

The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

Supplier: Agilent Technologies

The AAV helper-free system eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer.

Supplier: Enzo Life Sciences

pJY2 encodes tRNAUCUArg to suppress lysine misincorporation at AGA codons and T7 lysozyme to depress constitutive expression of the cloned gene. pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase.

Supplier: MP Biomedicals

pUC 18 is a small vector of approximately 2.7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.

Supplier: Agilent Technologies

The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

Supplier: Agilent Technologies

Combine standard DNA components to build custom vector constructs from Agilent’s set of standard parts.

Supplier: Agilent Technologies

The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.

Supplier: Cytiva

The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.

Supplier: Agilent Technologies

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

Supplier: Agilent Technologies

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.

Supplier: MilliporeSigma

The pET-28a-c(+) vectors carry an N-terminal His Tag/thrombin/T7 Tag configuration plus an optional C-terminal His Tag sequence.

Supplier: MP Biomedicals

pBR322 is a 4363 bp vector purified from Escherichia coli.

Supplier: MilliporeSigma

The pET-28a-c(+) vectors carry an N-terminal His Tag®/thrombin/T7 Tag® configuration plus an optional C-terminal His Tag sequence.

Supplier: MilliporeSigma

pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS), each of which is preceded by a T7 promoter, lac operator and ribosome binding sites.

Supplier: MilliporeSigma

The pET-21a vectors carry an N-terminal T7 Tag® sequence plus an optional C-terminal His Tag® sequence.

Supplier: MilliporeSigma

The pET-23a-d(+) vectors carry an N-terminal T7 Tag® sequence plus an optional C-terminal His Tag® sequence.

Supplier: New England Biolabs (NEB)

The single-stranded viral DNA is isolated from M13mp18

Supplier: New England Biolabs (NEB)

pSNAPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of SNAP-tag® protein fusions in mammalian cells

Supplier: Promega Corporation

pGL4.73[hRluc/SV40], pGL4.74[hRluc/TK] and pGL4.75[hRluc/CMV] Vectors are used as the control reporter for dual-luciferase assays. Select the appropriate promoter or test all three in your experimental system.

Supplier: Promega Corporation

The pGL4.10[luc2], pGL4.11[luc2P] and pGL4.12[luc2CP] Vectors are promoterless reporter vectors optimized for expression in mammalian cells. The different luc2 genes code for luciferase proteins with different stabilities.

Supplier: Promega Corporation

pGL4.26[luc2/minP/Hygro], pGL4.27[luc2P/minP/Hygro] and pGL4.28[luc2CP/minP/Hygro] Vectors are used to clone a response element of interest upstream of the minimal promoter and firefly luciferase gene with and without destabilization sequences.

Supplier: Promega Corporation

The pGL4.17[luc2/Neo] Vector, pGL4.18[luc2P/Neo] and pGL4.19[luc2CP/Neo] Vectors are optimized for expression in mammalian cells. These vectors offer standard and destablized luciferase with neomycin for stable selection.

Supplier: Promega Corporation

NanoLuc Stability Sensors are ready-to-use vector systems that utilize the advantages of the NanoLuc luciferase reporter for stability studies of two key signaling proteins, HIF1A and NRF2, providing a way to directly measure the primary signaling event.

Supplier: Promega Corporation

The pFN31 and pFC32 Vectors generate N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. Use pFN31A/K Nluc CMV-Hygro Flexi Vectors for N-terminal fusions and pFC32A/K Nluc CMV-Hygro Flexi Vectors for C-terminal fusions.

Supplier: Promega Corporation

Vectors in the sample pack are configured to append the HaloTag protein to either the carboxy- (pFC vectors) or amino-terminus (pFN vectors) of the protein fusion partner and provide constitutive protein expression in mammalian cells.

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) (Wuhan strain or Wild type strain), with 18-aa cytoplasmic tail truncation for optimal infection.

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Musoke (GenBank: DQ217792.1).