You searched for: Vectors, Plasmids and Libraries

Supplier: Edvotek

These plasmids allow you to perform bacterial transformations, conferring new antibiotic resistance, as a visual way of demonstrating gene transfer.

Supplier: Cytiva

Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.

Supplier: Agilent Technologies

The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.

Supplier: Cytiva

The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

Supplier: Agilent Technologies

The AAV helper-free system eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer.

Supplier: Enzo Life Sciences

pJY2 encodes tRNAUCUArg to suppress lysine misincorporation at AGA codons and T7 lysozyme to depress constitutive expression of the cloned gene. pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase.

Supplier: Agilent Technologies

The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.

Supplier: MP Biomedicals

pUC 18 is a small vector of approximately 2.7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.

Supplier: Agilent Technologies

Combine standard DNA components to build custom vector constructs from Agilent’s set of standard parts.

Supplier: Agilent Technologies

The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

Supplier: Agilent Technologies

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

Supplier: Agilent Technologies

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.

Supplier: Cytiva

The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.

Supplier: MilliporeSigma

The pET-28a-c(+) vectors carry an N-terminal His Tag®/thrombin/T7 Tag® configuration plus an optional C-terminal His Tag sequence.

Supplier: MilliporeSigma

pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS), each of which is preceded by a T7 promoter, lac operator and ribosome binding sites.

Supplier: MilliporeSigma

The pET-21a vectors carry an N-terminal T7 Tag® sequence plus an optional C-terminal His Tag® sequence.

Supplier: MilliporeSigma

The pET-23a-d(+) vectors carry an N-terminal T7 Tag® sequence plus an optional C-terminal His Tag® sequence.

Supplier: MP Biomedicals

pBR322 is a 4363 bp vector purified from Escherichia coli.

Supplier: MilliporeSigma

The pET-28a-c(+) vectors carry an N-terminal His Tag/thrombin/T7 Tag configuration plus an optional C-terminal His Tag sequence.

Supplier: Promega Corporation

Designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency.

Supplier: Promega Corporation

The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.

Supplier: Promega Corporation

The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.

Supplier: Promega Corporation

These untagged vectors are used for inducible expression of proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Supplied as vectors used with the Flexi Cloning System.

Supplier: Promega Corporation

The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.

Supplier: Promega Corporation

These multiple cloning site vectors are used for inducible expression of HaloTag-polyhistidine fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.

Supplier: Promega Corporation

These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.

Supplier: Promega Corporation

Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.

Supplier: Promega Corporation

Designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency.

Supplier: Promega Corporation

pBR322 Vector.

Supplier: Promega Corporation

The pGL4.10[luc2], pGL4.11[luc2P] and pGL4.12[luc2CP] Vectors are promoterless reporter vectors optimized for expression in mammalian cells. The different luc2 genes code for luciferase proteins with different stabilities.