You searched for: Enzymes

Supplier: Rockland Immunochemical

Collagenase Type 4

Supplier: Rockland Immunochemical

GST is purified by chromatography from E.coli preparations. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-GST antibody. Size: 100ug.

Supplier: MP Biomedicals

Soluble in water; Insoluble in ethanol, acetone and isopropyl alcohol.

Supplier: MP Biomedicals

One unit catalyzes the liberation of 1 mmole reducing group (calculated as maltose per minute at pH 6.9 at 20 °C).

Supplier: MP Biomedicals

Dissolves readily at 5 mg/mL in 0.05m sodium/potassium phosphate pH 7.0 giving a clear green solution.

Supplier: MP Biomedicals

Soluble in water; practically insoluble in alcohol and glycerol.

Supplier: Cytiva

A high specific activity, heat-labile alkaline phosphatase. Completely and irreversibly inactivated in Tris buffers at pH 8,0 to 8,5 by heating for 15 minutes at 65 °C. No further treatment is necessary.

Supplier: MP Biomedicals

One unit will hydrolyze 1 µmole of N-benzoyl-L-tyrosine ethyl ester per minute at pH 7.8 and 25 °C. Produced from 3× crystallized chymotrypsinogen.

Supplier: Cytiva

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).

Supplier: TCI America

CAS Number: 9001-77-8

Supplier: MP Biomedicals

Pig Pancreatin (from Pancreas), MP Biomedicals

Supplier: AGILENT TECHNOLOGIES, INC (CSD)

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL (formerly ProZyme). A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications). Supplied at a concentration of ≥ 2.5 U/mL. For a more concentrated formulation of ≥ 10 U/mL, which may be useful for native digestions, see GKE-5010B or GKE-5020B (EDTA-free).

Supplier: Enzo Life Sciences

Isolated from Apis mellifera.

Supplier: MP Biomedicals

Creatinine Deiminase (from Microbial), MP Biomedicals

Supplier: MP Biomedicals

Soluble in distilled water or dilute buffer.

Supplier: MP Biomedicals

1X N.F. Stable; 60 mesh powder

Supplier: Enzo Life Sciences

Produced in Sf9 cells. Fused at the C-terminus to a His-tag.

Supplier: Enzo Life Sciences

Produced in insect cells. Complex of human full length p110a and p85a subunits of phosphatidylinositol 3-kinase.

Supplier: Rockland Immunochemical

Collagenase Type 2

Supplier: MP Biomedicals

Collagenase can be solubilized in calcium-free solutions such as Hank's Balanced Salts or Earles Balanced Salt Solution.

Supplier: MP Biomedicals

The number of grams of soluble starch dextrinized in the presence of excess B-amylase per hour at 40 °C, pH 5.0.

Supplier: MP Biomedicals

CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7.0

Supplier: MP Biomedicals

One unit is the change in absorbancy of 1.00 per minute at 25 °C, pH 7.5.

Supplier: MP Biomedicals

One unit causes the formation of one µmole of hydrogen peroxide (half a µmole of quinoneimine dye) per minute at pH 5.7 at 37 °C.

Supplier: MP Biomedicals

Horseradish peroxidase (HRP) is isolated from horseradish roots and belongs to the ferroprotoporphyrin group of peroxidases. It is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme.

Supplier: MP Biomedicals

Proteinase K is a highly active stable endopeptidase with a broad spectrum of action was isolated by E. Merk's Darmstadt Biochemical Research Department in 1970 from a culture filtrate of the fungus, Tritirachium album Limber. This fungus is able to grow on Keratin (e.g., wool, horn particles) as the sole source of carbon and nitrogen. The isolated protease was, therefore, given the K designation.

Supplier: MP Biomedicals

Acetyl-CoA is produced via beta-oxidation of fatty acids, via the metabolism of carbohydrates - glucose 6-phosphate to pyruvate to acetyl-CoA and via the catabolism of amino acids. Acetyl-CoA has a number of metabolic opportunities. It is metabolized in the tricarboxylic acid cycle to produce carbon dioxide, water and energy.

Supplier: MP Biomedicals

Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The average chain of limit digest is a tetranucleotide. DNase I acts upon single chain DNA, and upon double-stranded DNA and chromatin. In the latter case, although histones restrict susceptibility to nuclease action, over a period of time nearly all chromatin DNA is acted upon. According to Mirsky and Silverman, this could result from the looseness of histone attachment to DNA. They found that lysine-rich histones more effectively block DNase access to DNA than arginine-rich histones. Billing and Bonner suggest that DNase attacks the histone-free strand of chromatin DNA. Schmidt, et. al.indicate that hydrolysis of the histone-free region of DNA strands accounts for the initial rapid action of the enzyme on chromatin. Bollum reports degradation of synthetic homopolymer complexes by DNase I. The intracellular functions of the enzyme are probably controlled by a DNase inhibitor, which according to Lazarides and Lindberg is actin.

Supplier: Enzo Life Sciences

Supplier: Enzo Life Sciences

Produced in E. coli.