Enzymes

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

J.T.Baker® Endonuclease Biotech Reagent meets the strictest cGMP standards and is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Biotech Reagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease Biotech Reagent is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. Absence of proteolytic activity. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Biotech Reagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.

Supplier: VWR

Pepsin is a proteolytic enzyme product derived from porcine stomach linings.

Supplier: VWR

Beta-D-Glucose oxidase with high purity grade and storage in frozen condition.

Supplier: MP Biomedicals

Alcohol oxidase has been detected in several strains of yeast,such as Candida, Pichia, and Hansenula,that utilize methanol as a sole carbon and energy source

Supplier: Sino Biological

Recombinant full-length human USP11 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Supplier: MP Biomedicals

Dilute in 10 mM Tris-HCl buffer, pH 8.0 containing 2 mM EDTA and 1.0% KCl.

Supplier: MP Biomedicals

Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The average chain of limit digest is a tetranucleotide. DNase I acts upon single chain DNA, and upon double-stranded DNA and chromatin. In the latter case, although histones restrict susceptibility to nuclease action, over a period of time nearly all chromatin DNA is acted upon. According to Mirsky and Silverman, this could result from the looseness of histone attachment to DNA. They found that lysine-rich histones more effectively block DNase access to DNA than arginine-rich histones. Billing and Bonner suggest that DNase attacks the histone-free strand of chromatin DNA. Schmidt, et. al.indicate that hydrolysis of the histone-free region of DNA strands accounts for the initial rapid action of the enzyme on chromatin. Bollum reports degradation of synthetic homopolymer complexes by DNase I. The intracellular functions of the enzyme are probably controlled by a DNase inhibitor, which according to Lazarides and Lindberg is actin.

Supplier: Enzo Life Sciences

Produced in Drosophila Schneider 2 (S2) cells. Recombinant human matriptase-2 (aa 78-811) is fused at the C-terminus to a V5-His-tag.

Supplier: TCI America

CAS Number: 9001-37-0

Supplier: MP Biomedicals

Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH).

Supplier: Sino Biological

Recombinant human LRRK2 (G2019S) (968-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Supplier: Thermo Scientific Chemicals

Powder

Supplier: MP Biomedicals

Activators: Papain is activated by cysteine, sulfide, sulfite, etc

Supplier: MP Biomedicals

Off-White Powder

Supplier: Enzo Life Sciences

Isolated from bovine kidney.

Supplier: MP Biomedicals

Sterile Aqueous Solution