You searched for: Enzymes

Supplier: MP Biomedicals

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. It is a lyophilized powder containing sucrose, sodium chloride and Tris. The predominant form of thrombin in vivo is the zymogen, prothrombin (factor II), which is produced in the liver. The concentration of prothrombin in normal human plasma is 5–10 mg/dL. Prothrombin is a glycoprotein with a glycan content of ~12%.

Supplier: MP Biomedicals

Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The average chain of limit digest is a tetranucleotide. DNase I acts upon single chain DNA, and upon double-stranded DNA and chromatin. In the latter case, although histones restrict susceptibility to nuclease action, over a period of time nearly all chromatin DNA is acted upon. According to Mirsky and Silverman, this could result from the looseness of histone attachment to DNA. They found that lysine-rich histones more effectively block DNase access to DNA than arginine-rich histones. Billing and Bonner suggest that DNase attacks the histone-free strand of chromatin DNA. Schmidt, et. al.indicate that hydrolysis of the histone-free region of DNA strands accounts for the initial rapid action of the enzyme on chromatin. Bollum reports degradation of synthetic homopolymer complexes by DNase I. The intracellular functions of the enzyme are probably controlled by a DNase inhibitor, which according to Lazarides and Lindberg is actin.

Supplier: MP Biomedicals

Horseradish peroxidase (HRP) is isolated from horseradish roots and belongs to the ferroprotoporphyrin group of peroxidases. It is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme.

Supplier: MP Biomedicals

Dissolves readily at 5 mg/mL in a 0.025M glychine/NaOH pH 9.6 containing 1 mM magnesium chloride, 0.1 mM zinc chloride and 10% glycerol, to give a clear pale pink solution also soluble in distilled water or dilute buffer.

Supplier: Cytiva

Exonuclease I acts specifically on single-stranded DNA degrading it processively in the 3'- to 5'-direction, producing 5'-mononucleotides. Applications include eliminating residual single-stranded DNA containing a 3'-terminus, measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA, measuring DNA helicase activity.

Supplier: MP Biomedicals

Alcohol oxidase has been detected in several strains of yeast,such as Candida, Pichia, and Hansenula,that utilize methanol as a sole carbon and energy source

Supplier: MP Biomedicals

Lysozyme (muramidase) hydrolyzes preferentially the b-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus.

Supplier: MP Biomedicals

Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The average chain of limit digest is a tetranucleotide. DNase I acts upon single chain DNA, and upon double-stranded DNA and chromatin.

Supplier: MP Biomedicals

Proteinase K is a highly active stable endopeptidase with a broad spectrum of action was isolated by E. Merk's Darmstadt Biochemical Research Department in 1970 from a culture filtrate of the fungus, Tritirachium album Limber. This fungus is able to grow on Keratin (e.g., wool, horn particles) as the sole source of carbon and nitrogen. The isolated protease was, therefore, given the K designation.

Supplier: Zymo Research

Zymo research DNase I is a recombinant endonuclease that cleaves single- and double-stranded DNA. It digests DNA more efficiently than other DNase I, making it optimal for sensitive applications like NGS (RNA-seq) and PCR. It is extensively purified, and quality controlled to ensure RNase-free and protease-free.

Supplier: MP Biomedicals

Ready-to-use proteinase K solution, 20 mg/ml in 10 mM Tris-HCl, 1 mM calcium acetate, pH 7.5 containing 50% glycerol.

Supplier: AGILENT TECHNOLOGIES, INC (CSD)

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL (formerly ProZyme). A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications). Supplied at a concentration of ≥ 2.5 U/mL. For a more concentrated formulation of ≥ 10 U/mL, which may be useful for native digestions, see GKE-5010B or GKE-5020B (EDTA-free).

Supplier: MP Biomedicals

Superoxide dismutases are enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide

Supplier: Bachem Americas

Sequence:

Supplier: Biolegend

Accutase® is a cell detachment solution containing proteolytic and collagenolytic enzymes.

Supplier: MilliporeSigma

Native Alkaline Phosphatase from calf intestine is a homodimeric enzyme that hydrolyzes phosphate esters or primary and secondary alcohols, amines and phenols

Supplier: Lucigen

ssDNA exonuclease with both 5´ to 3´ and 3´ to 5´ exonuclease activities

Supplier: MilliporeSigma

Effective viscosity reduction and removal of nucleic acids from protein solutions.

Supplier: Spectrum Chemicals

Subtilisin A, Aqueous Solution, a protein-digesting enzyme, can be obtained from Bacillus subtilis and is used in synthetic organic chemistry for research; it can also be found in dishwashing detergents and some cosmetics

Supplier: MilliporeSigma

Grx1

Supplier: MilliporeSigma

Effective viscosity reduction and removal of nucleic acids from protein solutions.

Supplier: MilliporeSigma

Sensitive fluorogenic substrate for the quantitative determination of thrombin.

Supplier: MilliporeSigma

From Trichoderma viride This enzyme shows a very high filter paper decomposing activity, as well as, appreciable xylanase and hemicellulase activity.

Supplier: Spectrum Chemicals

Commonly used in biochemistry.

Supplier: MilliporeSigma

In 50 mM Tris, pH 8.0

Supplier: MilliporeSigma

Convenient solution for selective degradation of RNA.

Supplier: Spectrum Chemicals

HRP. Peroxidase, Horseradish. CAS: 9003-99-0. Size: 25 KU.

Supplier: Peprotech

Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G Recombinant Mycoplasma Arginine Deiminase is a 46.3 kDa protein consisting of 409 amino acids.

Supplier: MP Biomedicals

A highly active stable endopeptidase with a broad spectrum of action was isolated by E.

Supplier: MilliporeSigma

Catalytic core enzyme of the 26S proteasome that degrades ubiquitinated proteins