You searched for: Enzymes

Supplier: MP Biomedicals

Bromelain is a glycoprotein which is a highly active thiol proteinase (non-specific cysteine protease), which splits glycine esters but not lysine esters; it is also a protein-digesting and milk-clotting enzyme. Substrates susceptible to bromelain include all of the common protein materials such as gelatin, casein, gluten, collagen, elastin, globulins and muscle fiber protein.

Supplier: MP Biomedicals

Glucose oxidase is an FAD-containing glycoprotein. The enzyme is specific for β-D-glucose. O can be replaced by hydrogen acceptors such as 2,6-dichlorophenol indophenol. Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation. Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates. Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.

Supplier: Rockland Immunochemical

Collagenase Type 3

Supplier: Enzo Life Sciences

Produced in insect cells. Active, full length p70 S6K.

Supplier: Enzo Life Sciences

ENZO Proteinase K is specifically designed for use in the pretreatment of formalin-fixed tissue and paraformaldehyde-fixed cells in preparation for in situ hybridization and detection studies.

Supplier: Enzo Life Sciences

Liquid. In 50mM TRIS-Cl, pH 7.5, containing 0.1M ATP and 0.1M MgCl2.

Supplier: MP Biomedicals

Soluble in water; Insoluble in ethanol, acetone and isopropyl alcohol.

Supplier: MP Biomedicals

One unit will oxidize 1.0 µmole of formaldehyde to formic acid per minute at pH 7.5 and 37 °C.

Supplier: MP Biomedicals

For Continuous Spectrophotometric Rate Determination method: 0.3 to 0.6 unit/ml of L-Glutamic dehydrogenase in cold Triethanolamine buffer.

Supplier: TCI America

Product Categories: Bioscience, Glycohydrolase, Hydrolases

Supplier: Rockland Immunochemical

Collagenase Type 4

Supplier: MP Biomedicals

Aprotinin is found in bovine lymph nodes, lung, parotid gland, spleen, liver, pancreas, seminal vesicles, thyroid gland, kidney, mucous membranes of the trachea and esophagus, ovaries, heart, posterior pituitary and cartilage. It is a serine protease inhibitor which is reactive against trypsin, chymotrypsin, plasmatic and glandular kininogenases, plasmin, kallkrein, urokinase, clotting factor XIIa, protein C, proteinases of the complement system, leukocyte and tissue proteinases. It does not inhibit thrombin. Aprotinin works by blocking the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes dissociating at pH > 10 or < 3.

Supplier: MP Biomedicals

Soluble in water; practically insoluble in alcohol and glycerol.

Supplier: MP Biomedicals

One unit converts one micromole of fructose-6-phosphate to glucose-6-phosphate per minute at pH 7.4 and 25 °C.

Supplier: AGILENT TECHNOLOGIES, INC (CSD)

The enzyme releases Alpha (2,3)-, Alpha (2,6)-, Alpha (2,8)-, and Alpha (2,9)-linked N-acetylneuraminic acid from oligosaccharides and glycoproteins. It is also capable of releasing N-glycolylneuraminic acid.

Supplier: Enzo Life Sciences

Produced in E. coli.

Supplier: Enzo Life Sciences

Produced in E. coli.

Supplier: TCI America

CAS Number: 9001-37-0

Supplier: MP Biomedicals

Dissolves readily at 5 mg/mL in a 0.025M glychine/NaOH pH 9.6 containing 1 mM magnesium chloride, 0.1 mM zinc chloride and 10% glycerol, to give a clear pale pink solution also soluble in distilled water or dilute buffer.

Supplier: MP Biomedicals

Lipases are a subclass of the esterases. It catalyzes the hydrolysis of emulsified esters of glycerol and long chain fatty acids.

Supplier: MP Biomedicals

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

Supplier: Enzo Life Sciences

Isolated from human plasma as prothrombin, and activated with Factor Xa, Factor Va, phospholipids, and calcium to yield a-thrombin.

Supplier: Corning

Dispase is a bacillus-derived neutral metalloprotease that is recommended for recovering cell cultured on Corning® Matrigel® Basement Membrane Matrix. Dispase will yield a single cell suspension far more gently and effectively than trypsin, collagenase, or other proteolytic enzymes; it will not harm cells harvested for sub cultivation or bioassays. In addition, Dispase may be used for tissue dissociation. Dispase cleaves fibronectin, collagen IV, and to a lesser extent collagen I, but is does not cleave collagen V or laminin.

Supplier: MP Biomedicals

One unit catalyzes the liberation of 1 mmole reducing group (calculated as maltose per minute at pH 6.9 at 20 °C).

Supplier: MP Biomedicals

1X N.F. Stable; 60 mesh powder

Supplier: Rockland Immunochemical

Suitable for use in peroxidase enzymatic labeling experiments.

Supplier: MP Biomedicals

Dissolves readily at 5 mg/mL in 0.05m sodium/potassium phosphate pH 7.0 giving a clear green solution.

Supplier: Rockland Immunochemical

GST is purified by chromatography from E.coli preparations. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-GST antibody. Size: 100ug.

Supplier: Enzo Life Sciences

Produced in Sf9 cells. Fused at the C-terminus to a His-tag.

Supplier: MP Biomedicals

Collagenase can be solubilized in calcium-free solutions such as Hank's Balanced Salts or Earles Balanced Salt Solution.