Competent Cells

Competent cells are those that have had their cell walls altered to render it easier to incorporate foreign DNA into their interiors. Cells are made competent by a process that uses calcium chloride and heat shock, and cells that are undergoing very rapid growth are made competent more easily than cells in other stages of growth. Factors such as transformation efficiency, genetic markers, and packaging formats will impact the time and effort required for your project, and so too its success. Choose from a number of cell types and formats to ensure optimal function.

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T7 Express Competent E.coli (High Effic.), New England Biolabs

Supplier: New England Biolabs (NEB)

Enhanced BL21 E.coli derivative for suitable for high efficiency transformation and protein expression.

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SCSI Supercompetent Cells, Agilent Technologies

Supplier: Agilent Technologies

SCSI supercompetent and DH5α competent cells are classic E. coli strains engineered for competence.

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SHuffle T7 Express Competent E. coli, New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E. coli cells suitable for T7 protein expression with enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm.

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dam-/dcm- Competent E.coli, New England Biolabs

Supplier: New England Biolabs (NEB)

Methyltransferase deficient chemically competent E.coli cells suitable for growth of plasmids free of Dam and Dcm methylation.

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K. lactis GG799 Competent Cells, New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent Kluyveromyces lactis cells suitable for transformation with a linearized pKLAC-series expression vector

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SHuffle T7 Express lysY Competent E. coli, New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E. coli cells suitable for T7 protein expression with enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm and with enhanced reduction of basal expression.

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SHuffle Express Competent E.coli, New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E. coli cells suitable for non-T7 protein expression with enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm.

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BL21 Competent Cells, Agilent Technologies

Supplier: Agilent Technologies

BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction.

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NEB®5-alpha Competent E.coli (Subcloning Efficiency), New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E.coli cells suitable for subcloning efficiency transformation in a wide variety of applications.

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NEB®Stable Competent E.coli (High Efficiency), New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E. coli cells suitable for high efficiency transformation and isolation of plasmid clones containing repeat elements.

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NEB®Turbo Competent E.coli (High Effic.), New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E.coli cells suitable for high efficiency transformation and rapid colony growth (6.5 hours).

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NEB®10-beta Competent E.coli (High Efficiency), New England Biolabs

Supplier: New England Biolabs (NEB)

NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high-quality plasmid preparations.

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NiCo21(DE3) Competent E.coli, New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E.coli cells derived from BL21(DE3).

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SHuffle T7 Competent E. coli, New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E. coli K12 cells suitable for T7 protein expression with enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm.

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Lemo21(DE3) Competent E. coli, New England Biolabs

Supplier: New England Biolabs (NEB)

Chemically competent E. coli cells suitable for tunable T7 expression of challenging proteins.

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T7 Express lysY/Iq Competent E.coli (High Efficiency), New England Biolabs

Supplier: New England Biolabs (NEB)

Enhanced BL21 E.coli derivative, Chemically competent E. coli cells suitable for high efficiency transformation and with the highest level of T7 expression control.