qScript™ XLT cDNA SuperMix, QuantaBio

qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR

• Stabilized 5X concentrated master mix simplifies reaction assembly
• Improved RNA template binding affinity for increased yields with problematic sequences or complex secondary structure
• Optimized, double pre-primed SuperMix provides equal representation of 5' and 3' RNA sequences

This 5X concentrated master mix provides all necessary components (except RNA template) for first-strand synthesis including buffer, dNTPs, MgCl2, primers, RNase inhibitor protein, qScript XLT reverse transcriptase and stabilizers. The unique blend of oligo (dT) and random primers in the qScript XLT cDNA SuperMix captures unbiased representation of all RNA sequences into cDNA product (including the 5’-end, 3’-end, or central regions of long RNAs) and works exceptionally well with a wide variety of RNA templates. However, due to the relatively short average length of random-primed first-strand product, amplicon length for conventional RT-PCR applications should be limited to 1 kb or less.

qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilizing an RNase H(+) reverse transcriptase (RT).