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144 results for Protein Extraction

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Protein Extraction

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

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InGel™ Blue for Protein Digestion, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' InGel™ Blue kit provides a complete set of reagents for the in gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS)

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FOCUS™ Glycoprotein, G-Biosciences

FOCUS™ Glycoprotein, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.

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Factor Xa Protease, New England Biolabs

Factor Xa Protease, New England Biolabs

Supplier: New England Biolabs (NEB)

Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg

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Protein Reducing Agents, G-Biosciences

Protein Reducing Agents, G-Biosciences

Supplier: G-Biosciences

A water soluble, odorless, non-toxic and stable protein reductant, G-Biosciences' Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol)

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Rabbit Reticulocyte Lysate, Untreated, 1ml, Promega

Rabbit Reticulocyte Lysate, Untreated, 1ml, Promega

Supplier: Promega Corporation

Untreated Rabbit Reticulocyte Lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease.

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B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

Supplier: Invitrogen

B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.

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Rabbit Reticulocyte Lysate System, Nuclease Treated, 30 reactions, Promega

Rabbit Reticulocyte Lysate System, Nuclease Treated, 30 reactions, Promega

Supplier: Promega Corporation

Rabbit Reticulocyte Lysate Translation Systems are used in identification of mRNA species, characterization of their protein products and investigation of transcriptional and translational control.

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ImmunoHistoZyme™, Ready-to-Use, Electron Microscopy Sciences

Supplier: Electron Microscopy Sciences

This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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Flexi Rabbit Reticulocyte Lysate System, 30 reactions, Promega

Flexi Rabbit Reticulocyte Lysate System, 30 reactions, Promega

Supplier: Promega Corporation

Flexi Rabbit Reticulocyte Lysate System allows optimization of translation reactions for a range of parameters, including Mg(2+) and K(+) and DTT concentration.

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HOOK™ Cell Surface Protein Isolation, Complete Labeling and Isolation Kit, G-Biosciences

HOOK™ Cell Surface Protein Isolation, Complete Labeling and Isolation Kit, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' HOOK™ Cell Surface Protein Isolation kit uses our HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting

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ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear), Bio-Rad

ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear), Bio-Rad

Supplier: Bio-Rad

The ReadyPrep protein extraction kit (cytoplasmic/nuclear) prepares fractions enriched in cytoplasmic and nuclear proteins from eukaryotic samples, such as cultured cells and tissues.

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ReadyPrep Protein Extraction Kit (Membrane II), Bio-Rad

ReadyPrep Protein Extraction Kit (Membrane II), Bio-Rad

Supplier: Bio-Rad

The ReadyPrep protein extraction kit (membrane II) applies sodium carbonate extraction methods (Fujiki et al

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Pierce™ TMTsixplex™ Protein Quantitation Kit and Label Reagent Sets, Thermo Scientific

Pierce™ TMTsixplex™ Protein Quantitation Kit and Label Reagent Sets, Thermo Scientific

Supplier: Invitrogen

Pierce™ TMT™ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry

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ReadyPrep Reagents for Extraction and Solubilization, Bio-Rad

ReadyPrep Reagents for Extraction and Solubilization, Bio-Rad

Supplier: Bio-Rad

A number of ReadyPrep kits are available for total protein or targeted protein extraction (fractionation)

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Pierce™ NE-PER® Nuclear and Cytoplasmic Extraction Reagent Kit, Thermo Scientific

Pierce™ NE-PER® Nuclear and Cytoplasmic Extraction Reagent Kit, Thermo Scientific

Supplier: Invitrogen

The NE-PER nuclear and cytoplasmic extraction kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours.

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Pierce™ Detoxi-Gel™ Endotoxin Removing Gel and Columns, Thermo Scientific

Pierce™ Detoxi-Gel™ Endotoxin Removing Gel and Columns, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Detoxi-Gel Endotoxin Removing Gel uses Polymixin B ligand immobilized on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.

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Pierce™ Surfact-Amps™ Detergent Solutions, Thermo Scientific

Pierce™ Surfact-Amps™ Detergent Solutions, Thermo Scientific

Supplier: Invitrogen

Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques.

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Brain Extract Polar, Avanti Polar Lipids

Supplier: Avanti Polar Lipids Inc

Polar lipid extract is derived from the total lipid extract by precipitation with acetone followed by extraction of the acetone insoluble material with diethyl ether.

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Pierce Imject™ Mariculture KLH Carrier Proteins, Thermo Scientific

Supplier: Invitrogen

Imject™ Mariculture KLH (mcKLH) is a purified keyhole limpet haemocyanin carrier protein that enables simple preparation of highly effective immunogens with peptide antigens.

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Pierce™ Organelle Enrichment Kits, Thermo Scientific

Pierce™ Organelle Enrichment Kits, Thermo Scientific

Supplier: Invitrogen

The lysosome enrichment kit for tissue and cultured cells enables isolation and enrichment for intact lysosomes from crude cell and tissue extracts. Upon receipt store at 4˚C. Product shipped at ambient temperature.

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Pierce™ Cell Surface Protein Isolation Kit, Thermo Scientific

Pierce™ Cell Surface Protein Isolation Kit, Thermo Scientific

Supplier: Invitrogen

The Pierce™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.

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Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific

Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific

Supplier: Invitrogen

The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.

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Pierce™ Ubiquitin Enrichment Kit, Thermo Scientific

Pierce™ Ubiquitin Enrichment Kit, Thermo Scientific

Supplier: Invitrogen

The Thermo Scientific Pierce Ubiquitin Enrichment Kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.

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Pierce™ Endoproteinase Chymotrypsin Protease (TLCK treated), MS Grade, Thermo Scientific

Pierce™ Endoproteinase Chymotrypsin Protease (TLCK treated), MS Grade, Thermo Scientific

Supplier: Invitrogen

Chymotrypsin Protease, MS Grade, is a purified native endoproteinase validated for maximum activity and selectivity in proteomic and used in in-solution digestions and in-gel digestion of proteins from from 1-D or 2-D gels. Store at -20˚C in a nonfrost-free freezer.

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Pierce™ Endoproteinase Asp-N Protease, MS Grade, Thermo Scientific

Pierce™ Endoproteinase Asp-N Protease, MS Grade, Thermo Scientific

Supplier: Invitrogen

Asp-N Protease, MS Grade, is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications. Store at -20˚C in a nonfrost-free freezer.

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Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific

Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific

Supplier: Invitrogen

Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling

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Pierce™ ProteoSeek™ Albumin/IgG Removal Kits, Thermo Scientific

Pierce™ ProteoSeek™ Albumin/IgG Removal Kits, Thermo Scientific

Supplier: Invitrogen

The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.

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Pierce Imject™ Maleimide-Activated Ovalbumin, Thermo Scientific

Supplier: Invitrogen

Imject™ Maleimide-activated ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.

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Pierce™ Trypsin Protease, MS Grade, Thermo Scientific

Pierce™ Trypsin Protease, MS Grade, Thermo Scientific

Supplier: Invitrogen

Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

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Pierce™ Albumin Depletion Kit, Thermo Scientific

Pierce™ Albumin Depletion Kit, Thermo Scientific

Supplier: Invitrogen

Pierce™ Albumin Depletion Kit improves serum component analysis by rapidly removing abundant albumin protein from serum samples

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