156 Results for: "pcr assay"

Supplier: Biotium

Viability PCR With PMA and PMAxx™ dyes

Supplier: VWR Collection

This robust cooler is consistent and reliable. It is made from engineered PP with a PC lid. The modern design cools faster in freezers and stays cold longer on the bench top.

Supplier: Thermo Fisher Scientific

Effective sealing of all microplate formats. For storage, PCR, microscopy, culture and protection.

Supplier: AGILENT

The MycoSensor PCR and qPCR assay kits detect cultured Mycoplasma infection by real-time qPCR or PCR.

Supplier: VWR Collection

Designed for pre- and post-PCR sampling, streamline your PCR workflow with the 32-Place PCR rack. This versatile rack accommodates 0,2 ml tubes both individually and in strips of 8, as well as 32-place assay plates (8×4).

Supplier: GOLD STANDARD DIAGNOSTICS

This RT-PCR assay is a highly sensitive Multiplex Real-Time PCR with CE-IVD marking for the direct qualitative pathogen detection of SARS-CoV-2 genomic RNA in respiratory samples.

Supplier: VWR Chemicals

PCR DNA Marker™ offers 8 fragments ranging from 50 to 2000 bp. The marker contains high intensity reference bands that are ideal for quick sizing of PCR products and restriction digests. Simply mix 10 μl of PCR DNA Marker™ with required quantity of loading buffer and load into well. There is sufficient material for 100 assays.

Supplier: EPPENDORF

Ensure airtight sealing of your PCR plates with Eppendorf® PCR films and foils. Tailor your choice to your assay needs, like the Masterclear® real-time PCR film, optimal for qPCR with its superior optical characteristics, or heat-sealing options for the best evaportion protection.

Supplier: GOLD STANDARD DIAGNOSTICS

This Real-Time RT-PCR assay is intended for the qualitative detection of SARS-CoV-2 from human nasopharyngeal swabs, eluted directly into deionised water without an additional RNA extraction process.

Supplier: AAT BIOQUEST

Detecting and quantitating small amounts of RNA is extremely important for a wide variety of biological applications such as measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.

Supplier: BIOPREMIER

SUPREME Real time detection uses real-time PCR for the detection of DNA present in the mitochondrial genome in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target.

Supplier: AAT BIOQUEST

Detecting and quantitating small amounts of RNA is extremely important for a wide variety of molecular biology procedures such as measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.

Supplier: AAT BIOQUEST

Detecting and quantitating small amounts of RNA is extremely important for a wide variety of molecular biology procedures such as measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.

Supplier: AAT BIOQUEST

Detecting and quantitating small amounts of RNA is extremely important for a wide variety of molecular biology procedures such as measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.

Supplier: VARIAN

The PLRP-S reversed phase HPLC media delivers the pure oligonucleotides (oligos) required for genomics applications, including quantitative PCR, microarrays, and gene synthesis assays.

Supplier: Quantabio

AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Supplier: SARSTEDT

Isofreeze® racks are for use as a pipetting and storage station for temperature sensitive applications like enzyme analyses, PCR or cell based assays. They prevent sample damage caused by premature warming.

Supplier: EPPENDORF

Eppendorf twin.tec® real-time PCR plates, designed for low-volume qPCR, utilise white wells to enhance fluorescent light reflection, improving sensitivity and reproducibility in assays for reliable results.

Supplier: Quantabio

AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II Taq DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Supplier: MEDICAL WIRE EQUIPMENT

Flocked swabs are ideal for PCR, molecular assays, forensic applications, food applications, and direct antigen testing. The bud is formed by a layer of tiny multi-filament polyester fibres of varying lengths, increasing the effective surface area of the swab.

Supplier: Quantabio

The 10 mM dNTP mix is a solution of high purity deoxyribonucleoside triphosphates that has been functionally qualified for real-time quantitative PCR (qPCR). It is suitable for use in conventional end-point PCR, real time qPCR, first-strand cDNA synthesis, as well as other applications that require dNTP as substrate. The use of qPCR certified dNTPs is a simple but important measure to ensure consistent assay performance.

Supplier: Quantabio

This SuperMix is a 2X concentrated, ready to use reaction cocktail for Real-Time qPCR that contains all components, except primers, probes, and templates. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.

Supplier: Merck Millipore (Novagen)

The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots, in situ hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant Thermus thermophilus (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

Supplier: Corning

FiltrEX™ filter plates meet the industry standards for plate dimensions. The rigid side walls make the plate ideal for automation and the wide skirt accepts barcodes. Individual filter discs are encapsulated in the plate by a process that ensures 100% integrity of each well. The design of the nozzle prevents sample wicking and cross-contamination. Glass fibre filter plates can be used for a variety of applications, such as plasmid isolation, DNA purification, PCR clean-up or receptor/ligand binding assays. The low-binding hydrophilic PVDF membrane can be used for lysate clarification, protein kinase assays, or bead- or resin-based separation assays.

Supplier: Corning

Axygen® PlateMax® semi automatic plate sealer is ideal for the low-to-medium throughput laboratory that requires uniform and consistent sealing of microplates. Offering complete versatility, the PlateMax® sealer will accept a full range of plates for PCR, assay or storage applications. It can be used to seal a wide range of plate heights. Sealing parameters are set and displayed via the user-friendly control panel and the sealing operation is automated to ensure consistent results.

Supplier: BIOPREMIER

Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of a specific pathogenic bacteria. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target microorganism. PCR is a method used to amplify a specific DNA sequence which is typically amplified in a reaction containing a thermostable DNA polymerase, nucleotides, and primers complementary to the target sequence. When this solution is heated, the DNA molecule denatures, separating into two strands. As the solution cools, the primers anneal to the target sequences in the separated DNA strands and the DNA polymerase synthesises a new strand by extending the primers with nucleotides, creating a copy of the DNA sequence (amplicons). When repeated, this cycle of denaturing, annealing, and extending exponentially increases the number of target amplicons. In Real-Time PCR, specific fluorescent probes are used to detect the amplified DNA by hybridising with amplicons. These probes are linked to a fluorophore on one end and a quencher that suppresses fluorescence on the other. If the target sequence is present during the PCR, amplification occurs and the probe is degraded, resulting in fluorescence increase. Fluorescence is measured by a detector and the associated software plots the fluorescence intensity versus number of cycles, allowing the determination of the presence or absence of the target organism.

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR low ROX mixes are optimised for use with real time instruments that are able to detect long wavelength dyes with greater sensitivity, such as the ABI 7500. The lower level of ROX still facilitates normalisation across the plate, while maintaining the sensitivity of the assay. The mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X qPCR mix contains a proprietary reaction buffer that provides high sensitivity, specificity and reproducibility.

Supplier: Greiner Bio-One

PS, without lid. Streptavidin coated solid phases serve as reliable binding surfaces for all types of biotinylated molecules. Numerous ligands can be biotinylated simply and reliably, and due to the low molecular weight of biotin (244 Da), the functionality of the molecules is normally not impaired. Thus streptavidin-coated solid phases make it possible to simply and rapidly isolate, determine and quantify components from a reaction mixture. By immobilising the biotinylated substance, it is also possible to reproduce complete reaction chains on a streptavidin solid phase, for example, enzyme immunoassays, enzyme activity assays, DNA hybridisation techniques, quantification of PCR products, and receptor/ligand studies.

Supplier: BIOPREMIER

Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of an animal species in meat and meat products. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target organism. According to European Commission Directive 2002/86/EC, food ingredients have to be declared. In the case of meat and meat products, the animal species has to be disclosed on the label. Moreover, the species autenticity can be highly relevant to consumers for economic, medical, cultural and religious reasons. Fraudulent substitution of cheaper meats in place of more expensive species, the inclusion of meat in non meat (vegetarian) products and the presence of allergens in food products are clear examples for the importance of this issue (Ballin, 2010; Dooley et al., 2004). Several methods are based on identification of proteins by means of electrophoretic and/or immunological methods. However, these methods are not reliable for application in highly processed and heated products due to the protein deterioration. DNA is more stable than proteins during processing and although it can be fragmented by several processes, modern DNA methodologies like PCR-based techniques still allow the identification of DNA from the different species present in a sample (Lockley & Bardsley, 2000). Real-Time PCR techniques are especially suitable for these products because small fragments of DNA can still be amplified and identified with high sensitivity and specificity (Dooley et al., 2004).

Supplier: Q BIOANALYTIC

OneCup it‘s so easy! The OneCup assay comes along with a minimum of pipetting steps. Everything is included in the mix. You only need to combine 20μl of the mix with 2 μl of your DNA sample and everything is ready. You have a minimum of hands-on time and you avoid mistakes during pipetting. You can run the test on all common block cyclers. Application: Detection of Listeria monocytogenes in food samples and environmental samples subsequent to a pre-enrichment step according to DIN EN ISO 20837 and 20838.Test principle. The test is dedicated to detect DNA of Listeria monocytogenes in a sample using Real-Time PCR in compliance with the ISO method mentioned below. The kit uses the TaqMan® principle. Thus the requirements of the standard, that an amplification product has to be confirmed by a hybridization step, is fulfilled. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons.