Supplier: AAT BIOQUEST

Cu-free click reaction, i.e., the strain-promoted alkyne-azide cycloaddition (SPAAC) is a bioorthogonal reaction utilizing a pair of reagents, cyclooctynes and azides that exclusively and efficiently react with each other while remain inert to naturally occurring functional groups such as hydroxy, amino and thiol groups.

Supplier: Thermo Fisher Scientific

Mechanical Seals, Graphite liner sealing ring for Agilent SSL liners

Supplier: AAT BIOQUEST

Fluorescence-based methods have many advantages for biological detections in terms of sensitivity.

Supplier: AAT BIOQUEST

ReadiCleave™ AML XFD532 contains an azidomethyl linker that can be cleaved with TCEP to remove the XFD532 fluorophore from the target molecule.

Supplier: AAT BIOQUEST

ReadiCleave™ AML FITC contains an azidomethyl linker that can be cleaved with TCEP to remove the fluorescein fluorophore from the target molecule.

Supplier: AAT BIOQUEST

This Cy7® dye is the same molecule to GE's monoreactive Cy7® NHS ester.

Supplier: AAT BIOQUEST

ReadiCleave™ iFluor® 546 AML contains an azidomethyl linker that can be cleaved with TCEP to remove the iFluor® 546 fluorophore from the target molecule.

Supplier: AAT BIOQUEST

ReadiCleave™ iFluor® 594 AML uses an azidomethyl linker that can be cleaved with TCEP to remove the iFluor® 594 fluorophore from the target molecule.

Supplier: AAT BIOQUEST

Cu-free click reaction, i.e., the strain-promoted alkyne-azide cycloaddition (SPAAC) is a bioorthogonal reaction utilizing a pair of reagents, cyclooctynes and azides that exclusively and efficiently react with each other while remain inert to naturally occurring functional groups such as hydroxy, amino and thiol groups.

Supplier: AAT BIOQUEST

6-VIC, SE is a popular amino-reactive fluorescent probe that is widely used in nucleic acid sequencing and related research.

Supplier: AAT BIOQUEST

ReadiCleave™ AML iFluor® 488 contains an azidomethyl linker that can be cleaved with TCEP to remove the iFluor® 488 fluorophore from the target molecule.

Supplier: AAT BIOQUEST

Single isomers of ROX are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purifications that are often required in bioconjugations.

Supplier: AAT BIOQUEST

ATTO 647 is a rhodamine-derived fluorescent dye optimized for applications within the red spectral region, offering spectral characteristics similar to Cy5.

Supplier: AAT BIOQUEST

Bodi Fluor™ 576/589 is chemically the same molecule to BODIPY™ 576/589 dye.

Supplier: STEMCELL Technologies

CFDA-SE (5(6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE.

Supplier: AAT BIOQUEST

ATTO 532 is a rhodamine-based fluorescent labeling dye with high molecular high extinction coefficient and fluorescence quantum yield (0.90) as well as sufficient Stoke's shift (excitation maximum 532 nm, emission maximum 552 nm).

Supplier: Cytiva

These versatile fluorophores can tolerate a pH range of 3 - 10 for use in a variety of applications at biologically relevant pHs. The dyes are also DMSO tolerant and photostable to enable transfer from storage to assay without loss of performance. The aqueous solubility eliminates the need for organic solvents in the assay buffers. Fluors are thoroughly QC tested to ensure high levels of chromophore and reactive dye content.

Supplier: AAT BIOQUEST

It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample.

Supplier: AAT BIOQUEST

This Cy5® dye is the same molecule to GE's monoreactive Cy5® NHS ester.

Supplier: AAT BIOQUEST

A variety of cyanine 5.5 (Cy5.5®) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.

Supplier: AAT BIOQUEST

ReadiUse™ Preactivated HRP NHS ester is the mono NHS ester of HRP.

Supplier: AAT BIOQUEST

This Cy5.5® dye is the same molecule to GE's monoreactive Cy5.5® NHS ester.

Supplier: AAT BIOQUEST

This Cy3.5® dye is the same molecule to GE's Cy3.5® NHS ester.

Supplier: AAT BIOQUEST

This Cy3® dye is the same molecule to GE's Cy3® NHS ester.

Supplier: AAT BIOQUEST

It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample.

Supplier: AAT BIOQUEST

Cy7.5 dyes undergo comprehensive quality control testing to ensure high levels of chromophore and reactive dye content.

Supplier: AAT BIOQUEST

The acridinium NHS ester can be used to label proteins and nucleic acids.

Supplier: AAT BIOQUEST

A variety of cyanine 3.5 (Cy3.5®) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.

Supplier: Thermo Fisher Scientific

NHS fluorescein [(5/6-carboxyfluorescein succinimidyl ester), mixed isomer] is an amine reactive derivative of fluorescein dye for antibodies and other probes in fluorescence microscopy, flow cytometry, and immunofluorescence based assays such as western blotting and ELISA.

Supplier: Merck

EMCS and its water-soluble analog Sulfo-EMCS are heterobifunctional cross-linkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugation experiments involving these cross-linkers are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.