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Silver Protein

Supplier: SIGMA ALDRICH MICROSCOPY

Protargol silver impregnation and neuronal selectivity for light and electron microscopic observations.

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o-Tolidine ≥95%

Supplier: SIGMA ALDRICH MICROSCOPY

o-Tolidine is a diacidic dye which is sparingly soluble in water and easily soluble in ether and alcohol. On exposure to chlorine or bromine vapors, it forms an intense blue color. This forms the basis for detection of chlorine, nitrates and hydrogen peroxide in air or water. It turns yellow on reacting with free chlorine. o-Tolidine easily forms acid salts.

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Methylene blue solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Loefflers Methylene Blue stain is used for the identification of diphteria bacilli, since it differentiates the deeply staining metachromatic granules from the pale blue-staining cytoplasm.

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Naphthol AS-GR phosphate disodium salt

Supplier: SIGMA ALDRICH MICROSCOPY

Produces highly insoluble dark green stain in histochemical studies.

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Percoll®, Siam

Supplier: SIGMA ALDRICH MICROSCOPY

Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. It consists of colloidal silica particles of 15 – 30 nm diameter (23% w/w in water), coated with polyvinylpyrrolidone (PVP).

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Azure A eosinate, powder, crystals or chunks, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Azure A eosinate, powder, crystals or chunks, Sigma-Aldrich®

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2-Amino-5,6-dimethylbenzimidazole, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

2-Amino-5,6-dimethylbenzimidazole, Sigma-Aldrich®

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Crystal violet solution Grams for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Gram’s crystal violet solution, also known as gentian violet or hexamethyl pararosaniline, is a basic, cationic, acidotropic aminoarylmethane dye. It is a hexa-N-methylated triaminotriphenylmethane with a moderately sized lipophilic cation and a slightly nonplanar conjugated system. It is used in bacteriology as the primary stain in Gram staining to differentiate Gram-positive and Gram-negative bacteria. It is known to possess antibacterial and antifungal properties.

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Nile blue chloride (Basic blue 12) 85% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.

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Tartrazine, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Yellow counterstain suitable for a wide range of histological staining procedures.

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Reactive black 5 ≥50 % (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Reactive Black 5 (RB5) has been: used in an optimization study on the photocatalytic degradation of RB5 dye in synthetic greywater effluent (i.e. aqueous RB5 in distilled water), in order to understand the effects of different photoreactor parameters on the degradation kinetics. As a textile dye to investigate the anaerobic reduction in upflow stirred packed-bed reactors (USPBRs) with biological activated carbon (BAC) system. Used in the performance evaluation of dye removal forlarge molecule substance.

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Papanicolaou's solution EA 65, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Papanicolaou stain imparts a characteristic range of coloration to exfoliative cells of vaginal, cervical, prostatic and other body secretions, allowing critical examination of nuclei and cytoplasmic components.

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Silver methenamine

Silver methenamine

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for use in the silver stain (modified GMS) procedure.

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Methyl violet 2B for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Methyl violet for microscopy (Bact., Bot., Hist.), indicator (pH 0.1 - 2.0), mixture of polymethylated pararosaniline hydrochlorides.

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Carmine, powder, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Carmine is a red compound and is generally used with aluminum, iron and other metal salts to enhance its activity. During smear preparation, it is required for chromatin staining in fresh material. In presence of aluminum chloride, it is used for the staining of glycogen.

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Neutral red solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Used as a red nuclear counterstain in various cytochemical procedures.

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Quinoline yellow sulphonic acid sodium salt (Acid yellow 3), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Quinoline Yellow, Mixture of the mono- and disulfonic acids of Quinoline Yellow (Acid Yellow 3)

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FURA-2 pentapotassium salt, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Cell impermeable fluorescent probe for Ca2+.

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Solvent Blue 38, Practical grade, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Solvent Blue 38 is a phthalocyanine stain, used for staining myelin, neurons and glial fibres.

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Acridine orange base ≥75% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye. Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.

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Rose Bengal 95% (dye content) ≥96% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Rose Bengal has been used to evaluate acrosome integrity. It has also been used to initiate photothrombotic occlusion in blood.

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5-Aminofluorescein (isomer I), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoresceinamine, isomer I has been used as a probe in immobilization and is used to determine its ability to potentiate the antiviral activity of poly r (A-U).

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1-Methoxy-5-methylphenazinium methyl sulphate ≥95%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

1-Methoxy-5-methylphenazinium methyl sulfate (MPMS) is a photochemically stable electron-transport mediator between NAD(P)H and tetrazolium dyes. The reduced form of MPMS converts tetrazolium salt to purple precipitate formazan. It is a very stable and non-enzymic electron carrier that mediates electron transfer by dehydrogenases in vitro to various electron acceptors such as tetrazolium dyes or the electrode of an enzymic electric cell.

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Rhodamine 123 fluorescent dye, mitochondria dye

Supplier: SIGMA ALDRICH MICROSCOPY

Rhodamine 123 is useful as a laser dye and to have selective cell growth effects. It has also been used to measure mitochondrial membrane potential.

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Fast Red Violet LB Salt ≥90% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fast Red Violet LB Salt has been used for the detection of tartrate-resistant acid phosphatase (TRAP) in tissue sections.

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bisbenzimide H-33342 trihydrochloride (Hoechst 33342) ≥98% (by HPLC, TLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
bisBenzimide H 33342 trihydrochloride has been used for the staining of viable and apoptotic.

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Cresyl violet perchlorate, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Cresyl Violet perchlorate is suitable as a laser dye.

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Acid Phosphatase, Leukocyte (TRAP) Kit Kit formulated with all liquid reagents, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Kit is used to demonstrate acid phosphatase and tartrate resistant acid phosphatase (TRAP) in blood, bone marrow and tissue touch preparations.

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Chlorazol black E, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Chlorazol Black has been used to monitor the infection of mycorrhizal Allium porrum in plant roots. It has also been used for the staining of copepods.

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Arsenazo III for calcium determination, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Arsenazo III has been used as a color forming reagent to determine uranium concentration.

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